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Suitable housekeeping genes for normalization of transcript abundance analysis by real-time RT-PCR in cultured bovine granulosa cells during hypoxia and differential cell plating density
BACKGROUND: Bovine granulosa cell culture models are important to understand molecular mechanisms of ovarian function. Folliculogenesis and luteinization are associated with increasing density of cells and local hypoxic conditions. The current study identified two reliable housekeeping genes useful...
Autores principales: | Baddela, Vijay S, Baufeld, Anja, Yenuganti, Vengala R, Vanselow, Jens, Singh, Dheer |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4280684/ https://www.ncbi.nlm.nih.gov/pubmed/25430436 http://dx.doi.org/10.1186/1477-7827-12-118 |
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