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Preventive effect of pentoxifylline on contrast-induced acute kidney injury in hypercholesterolemic rats
Oxidative stress is an important mechanism of contrast-induced acute kidney injury (CIAKI). The optimal strategy to prevent CIAKI remains unclear. The aim of the present study was to assess the effect of pentoxifylline, a nonspecific phosphodiesterase inhibitor, on the prevention of CIAKI. A total o...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
D.A. Spandidos
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4280922/ https://www.ncbi.nlm.nih.gov/pubmed/25574202 http://dx.doi.org/10.3892/etm.2014.2132 |
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author | YANG, SHI-KUN DUAN, SHAO-BIN PAN, PENG XU, XIANG-QING LIU, NA XU, JUN |
author_facet | YANG, SHI-KUN DUAN, SHAO-BIN PAN, PENG XU, XIANG-QING LIU, NA XU, JUN |
author_sort | YANG, SHI-KUN |
collection | PubMed |
description | Oxidative stress is an important mechanism of contrast-induced acute kidney injury (CIAKI). The optimal strategy to prevent CIAKI remains unclear. The aim of the present study was to assess the effect of pentoxifylline, a nonspecific phosphodiesterase inhibitor, on the prevention of CIAKI. A total of 32 healthy male Sprague-Dawley rats were randomly divided into normal dietary group (NN; n=8) and a high cholesterol-supplemented dietary group (HN; 4% cholesterol and 1% cholic acid; n=24). At the end of eight weeks, the rats in the high cholesterol diet group were randomly divided into three subgroups (n=8 in each group). CIAKI was induced in two of the subgroups via intravenous injection of the radiocontrast media iohexol (10 ml/kg). Pentoxifylline (50 mg/kg) was administered to one of the iohexol-treated groups via intraperitoneal injection 12 h prior to and following contrast media (CM) injection. Kidney function parameters and oxidative stress markers were then measured. The renal pathological changes were evaluated using hematoxylin and eosin staining and scored semi-quantitatively. In iohexol-injected rats, serum creatinine (Scr), renal pathological scores, renal malondialdehyde (MDA) content, renal NADPH oxidase activity, fractional excretion of sodium (FENa%) and fractional excretion of potassium (FEK%) were significantly increased (P<0.01). The Scr, histologic scores, renal MDA content, NADPH oxidase activity, FENa% and FEK% in the rats treated with pentoxifylline prior to iohexol were observed to be reduced compared with those in rats treated with iohexol alone (P<0.01). This suggests that pentoxifylline significantly attenuates renal injuries, including tubular necrosis and proteinaceous casts induced by CM. It may be concluded that pentoxifylline protected the renal tissue from the nephrotoxicity induced by low-osmolar CM via an antioxidant effect. |
format | Online Article Text |
id | pubmed-4280922 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | D.A. Spandidos |
record_format | MEDLINE/PubMed |
spelling | pubmed-42809222015-01-08 Preventive effect of pentoxifylline on contrast-induced acute kidney injury in hypercholesterolemic rats YANG, SHI-KUN DUAN, SHAO-BIN PAN, PENG XU, XIANG-QING LIU, NA XU, JUN Exp Ther Med Articles Oxidative stress is an important mechanism of contrast-induced acute kidney injury (CIAKI). The optimal strategy to prevent CIAKI remains unclear. The aim of the present study was to assess the effect of pentoxifylline, a nonspecific phosphodiesterase inhibitor, on the prevention of CIAKI. A total of 32 healthy male Sprague-Dawley rats were randomly divided into normal dietary group (NN; n=8) and a high cholesterol-supplemented dietary group (HN; 4% cholesterol and 1% cholic acid; n=24). At the end of eight weeks, the rats in the high cholesterol diet group were randomly divided into three subgroups (n=8 in each group). CIAKI was induced in two of the subgroups via intravenous injection of the radiocontrast media iohexol (10 ml/kg). Pentoxifylline (50 mg/kg) was administered to one of the iohexol-treated groups via intraperitoneal injection 12 h prior to and following contrast media (CM) injection. Kidney function parameters and oxidative stress markers were then measured. The renal pathological changes were evaluated using hematoxylin and eosin staining and scored semi-quantitatively. In iohexol-injected rats, serum creatinine (Scr), renal pathological scores, renal malondialdehyde (MDA) content, renal NADPH oxidase activity, fractional excretion of sodium (FENa%) and fractional excretion of potassium (FEK%) were significantly increased (P<0.01). The Scr, histologic scores, renal MDA content, NADPH oxidase activity, FENa% and FEK% in the rats treated with pentoxifylline prior to iohexol were observed to be reduced compared with those in rats treated with iohexol alone (P<0.01). This suggests that pentoxifylline significantly attenuates renal injuries, including tubular necrosis and proteinaceous casts induced by CM. It may be concluded that pentoxifylline protected the renal tissue from the nephrotoxicity induced by low-osmolar CM via an antioxidant effect. D.A. Spandidos 2015-02 2014-12-15 /pmc/articles/PMC4280922/ /pubmed/25574202 http://dx.doi.org/10.3892/etm.2014.2132 Text en Copyright © 2015, Spandidos Publications http://creativecommons.org/licenses/by/3.0 This is an open-access article licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported License. The article may be redistributed, reproduced, and reused for non-commercial purposes, provided the original source is properly cited. |
spellingShingle | Articles YANG, SHI-KUN DUAN, SHAO-BIN PAN, PENG XU, XIANG-QING LIU, NA XU, JUN Preventive effect of pentoxifylline on contrast-induced acute kidney injury in hypercholesterolemic rats |
title | Preventive effect of pentoxifylline on contrast-induced acute kidney injury in hypercholesterolemic rats |
title_full | Preventive effect of pentoxifylline on contrast-induced acute kidney injury in hypercholesterolemic rats |
title_fullStr | Preventive effect of pentoxifylline on contrast-induced acute kidney injury in hypercholesterolemic rats |
title_full_unstemmed | Preventive effect of pentoxifylline on contrast-induced acute kidney injury in hypercholesterolemic rats |
title_short | Preventive effect of pentoxifylline on contrast-induced acute kidney injury in hypercholesterolemic rats |
title_sort | preventive effect of pentoxifylline on contrast-induced acute kidney injury in hypercholesterolemic rats |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4280922/ https://www.ncbi.nlm.nih.gov/pubmed/25574202 http://dx.doi.org/10.3892/etm.2014.2132 |
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