Cargando…

Effects of a nutrient mixture on immunohistochemical localization of cancer markers in human cervical cancer HeLa cell tumor xenografts in female nude mice

Although fully treatable in the early stages, once cervical cancer has metastasized, patient outcome is poor. The main objective of this study was to examine the effect of dietary supplementation with a nutrient mixture (NM) containing lysine, ascorbic acid, proline, green tea extract and other micr...

Descripción completa

Detalles Bibliográficos
Autores principales: ROOMI, M.W., KALINOVSKY, T., CHA, J., ROOMI, N.W., NIEDZWIECKI, A., RATH, M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: D.A. Spandidos 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4280926/
https://www.ncbi.nlm.nih.gov/pubmed/25574189
http://dx.doi.org/10.3892/etm.2014.2127
_version_ 1782350917519015936
author ROOMI, M.W.
KALINOVSKY, T.
CHA, J.
ROOMI, N.W.
NIEDZWIECKI, A.
RATH, M.
author_facet ROOMI, M.W.
KALINOVSKY, T.
CHA, J.
ROOMI, N.W.
NIEDZWIECKI, A.
RATH, M.
author_sort ROOMI, M.W.
collection PubMed
description Although fully treatable in the early stages, once cervical cancer has metastasized, patient outcome is poor. The main objective of this study was to examine the effect of dietary supplementation with a nutrient mixture (NM) containing lysine, ascorbic acid, proline, green tea extract and other micronutrients on HeLa cell xenografts in nude female mice. Tumor growth was measured and immunohistochemical staining was evaluated for the following cancer markers: Ki67 (proliferation); matrix metalloproteinase (MMP)-2 and -9 (invasion/metastasis); vascular endothelial growth factor (VEGF) (angiogenesis); terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) and B-cell lymphoma 2 (Bcl-2) (apoptosis); cyclooxygenase 2 (COX-2) and inducible nitric oxide synthase (iNOS) (inflammation); and glutathione S-transferase π (GSTπ) (a general cancer marker). Following housing for a week, 5/6-week-old female athymic nude mice (n=12) were inoculated subcutaneously with 3×10(6) HeLa cells in 0.2 ml phosphate-buffered saline and 0.1 ml Matrigel™ and randomly divided into two groups; control group mice were fed regular mouse chow and NM group mice the regular diet supplemented with 0.5% NM (w/w). After four weeks, the mice were sacrificed and their tumors were excised and processed for histology. The NM strongly inhibited the growth of HeLa xenografts in nude mice. The mean tumor weight was reduced to 59% (P=0.001) in the mice fed the NM compared with the tumor weight in the controlled diet mice. Ki67, MMP-2 and -9, VEGF, TUNEL, Bcl-2, COX-2, iNOS and GSTπ all showed a lower intensity and frequency of staining in the NM group compared with that in the control group. In conclusion, NM supplementation strongly inhibited tumor growth and cancer markers in female nude mice injected with HeLa xenografts.
format Online
Article
Text
id pubmed-4280926
institution National Center for Biotechnology Information
language English
publishDate 2015
publisher D.A. Spandidos
record_format MEDLINE/PubMed
spelling pubmed-42809262015-01-08 Effects of a nutrient mixture on immunohistochemical localization of cancer markers in human cervical cancer HeLa cell tumor xenografts in female nude mice ROOMI, M.W. KALINOVSKY, T. CHA, J. ROOMI, N.W. NIEDZWIECKI, A. RATH, M. Exp Ther Med Articles Although fully treatable in the early stages, once cervical cancer has metastasized, patient outcome is poor. The main objective of this study was to examine the effect of dietary supplementation with a nutrient mixture (NM) containing lysine, ascorbic acid, proline, green tea extract and other micronutrients on HeLa cell xenografts in nude female mice. Tumor growth was measured and immunohistochemical staining was evaluated for the following cancer markers: Ki67 (proliferation); matrix metalloproteinase (MMP)-2 and -9 (invasion/metastasis); vascular endothelial growth factor (VEGF) (angiogenesis); terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) and B-cell lymphoma 2 (Bcl-2) (apoptosis); cyclooxygenase 2 (COX-2) and inducible nitric oxide synthase (iNOS) (inflammation); and glutathione S-transferase π (GSTπ) (a general cancer marker). Following housing for a week, 5/6-week-old female athymic nude mice (n=12) were inoculated subcutaneously with 3×10(6) HeLa cells in 0.2 ml phosphate-buffered saline and 0.1 ml Matrigel™ and randomly divided into two groups; control group mice were fed regular mouse chow and NM group mice the regular diet supplemented with 0.5% NM (w/w). After four weeks, the mice were sacrificed and their tumors were excised and processed for histology. The NM strongly inhibited the growth of HeLa xenografts in nude mice. The mean tumor weight was reduced to 59% (P=0.001) in the mice fed the NM compared with the tumor weight in the controlled diet mice. Ki67, MMP-2 and -9, VEGF, TUNEL, Bcl-2, COX-2, iNOS and GSTπ all showed a lower intensity and frequency of staining in the NM group compared with that in the control group. In conclusion, NM supplementation strongly inhibited tumor growth and cancer markers in female nude mice injected with HeLa xenografts. D.A. Spandidos 2015-02 2014-12-11 /pmc/articles/PMC4280926/ /pubmed/25574189 http://dx.doi.org/10.3892/etm.2014.2127 Text en Copyright © 2015, Spandidos Publications http://creativecommons.org/licenses/by/3.0 This is an open-access article licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported License. The article may be redistributed, reproduced, and reused for non-commercial purposes, provided the original source is properly cited.
spellingShingle Articles
ROOMI, M.W.
KALINOVSKY, T.
CHA, J.
ROOMI, N.W.
NIEDZWIECKI, A.
RATH, M.
Effects of a nutrient mixture on immunohistochemical localization of cancer markers in human cervical cancer HeLa cell tumor xenografts in female nude mice
title Effects of a nutrient mixture on immunohistochemical localization of cancer markers in human cervical cancer HeLa cell tumor xenografts in female nude mice
title_full Effects of a nutrient mixture on immunohistochemical localization of cancer markers in human cervical cancer HeLa cell tumor xenografts in female nude mice
title_fullStr Effects of a nutrient mixture on immunohistochemical localization of cancer markers in human cervical cancer HeLa cell tumor xenografts in female nude mice
title_full_unstemmed Effects of a nutrient mixture on immunohistochemical localization of cancer markers in human cervical cancer HeLa cell tumor xenografts in female nude mice
title_short Effects of a nutrient mixture on immunohistochemical localization of cancer markers in human cervical cancer HeLa cell tumor xenografts in female nude mice
title_sort effects of a nutrient mixture on immunohistochemical localization of cancer markers in human cervical cancer hela cell tumor xenografts in female nude mice
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4280926/
https://www.ncbi.nlm.nih.gov/pubmed/25574189
http://dx.doi.org/10.3892/etm.2014.2127
work_keys_str_mv AT roomimw effectsofanutrientmixtureonimmunohistochemicallocalizationofcancermarkersinhumancervicalcancerhelacelltumorxenograftsinfemalenudemice
AT kalinovskyt effectsofanutrientmixtureonimmunohistochemicallocalizationofcancermarkersinhumancervicalcancerhelacelltumorxenograftsinfemalenudemice
AT chaj effectsofanutrientmixtureonimmunohistochemicallocalizationofcancermarkersinhumancervicalcancerhelacelltumorxenograftsinfemalenudemice
AT roominw effectsofanutrientmixtureonimmunohistochemicallocalizationofcancermarkersinhumancervicalcancerhelacelltumorxenograftsinfemalenudemice
AT niedzwieckia effectsofanutrientmixtureonimmunohistochemicallocalizationofcancermarkersinhumancervicalcancerhelacelltumorxenograftsinfemalenudemice
AT rathm effectsofanutrientmixtureonimmunohistochemicallocalizationofcancermarkersinhumancervicalcancerhelacelltumorxenograftsinfemalenudemice