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Effect of extractions from Ephedra sinica Stapf on hyperlipidemia in mice
The aim of the present study was to investigate the hypolipidemic and antioxidant potential of ephedra extractions in diet-induced hyperlipidemic mice. Mice were fed a diet high in fat to establish the hyperlipidemic model. A total of 48 mice were randomly divided into six groups, which included the...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
D.A. Spandidos
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4280985/ https://www.ncbi.nlm.nih.gov/pubmed/25574244 http://dx.doi.org/10.3892/etm.2014.2117 |
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author | FAN, YANBO LI, JINGJING YIN, QIANG ZHANG, YISHENG XU, HUIFANG SHI, XINHUA LI, CHEN ZHOU, YAN ZHOU, CAIXIN |
author_facet | FAN, YANBO LI, JINGJING YIN, QIANG ZHANG, YISHENG XU, HUIFANG SHI, XINHUA LI, CHEN ZHOU, YAN ZHOU, CAIXIN |
author_sort | FAN, YANBO |
collection | PubMed |
description | The aim of the present study was to investigate the hypolipidemic and antioxidant potential of ephedra extractions in diet-induced hyperlipidemic mice. Mice were fed a diet high in fat to establish the hyperlipidemic model. A total of 48 mice were randomly divided into six groups, which included the normal control, model control, positive control, ephedra alkaloid, ephedra polysaccharide and ephedra non-alkaloid groups. Intragastric administration of the respective treatments was provided continuously for four weeks and the body weight was recorded weekly. The total levels of cholesterol (TC), triglycerides (TG), high-density lipoprotein cholesterol (HDL-C) and malondialdehyde (MDA), and the activity levels of superoxide dismutase (SOD), alanine aminotransferase (ALT) and aspartate aminotransferase (AST) in the serum were recorded. In addition, changes in liver morphology and organ coefficients (ratio of organ to body weight) were evaluated, while the acute toxicity reactions of ephedra extractions were investigated using the modified Spearman-Karber method. Compared with the mice in the model control group, the weight, liver coefficient, serum levels of TC, TG and MDA, and activities of ALT and AST were significantly lower (P<0.05) in the mice in the ephedra non-alkaloid group. However, the level of HDL-C and the activity of SOD were markedly higher (P<0.05). Fatty degeneration of the liver in the ephedra alkaloid and non-alkaloid groups was notably improved compared with the model control group. The mean lethal dose (LD(50)) of ephedra alkaloids was 610 mg/kg, and the maximum tolerated dose of oral ephedra non-alkaloids in the mice was 367.5-fold larger than the clinical dosage in humans. In conclusion, ephedra non-alkaloids have therapeutic potential for the treatment of hyperlipidemia, since they are able to improve lipid metabolism and are relatively safe for use under the maximum tolerated dose. |
format | Online Article Text |
id | pubmed-4280985 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | D.A. Spandidos |
record_format | MEDLINE/PubMed |
spelling | pubmed-42809852015-01-08 Effect of extractions from Ephedra sinica Stapf on hyperlipidemia in mice FAN, YANBO LI, JINGJING YIN, QIANG ZHANG, YISHENG XU, HUIFANG SHI, XINHUA LI, CHEN ZHOU, YAN ZHOU, CAIXIN Exp Ther Med Articles The aim of the present study was to investigate the hypolipidemic and antioxidant potential of ephedra extractions in diet-induced hyperlipidemic mice. Mice were fed a diet high in fat to establish the hyperlipidemic model. A total of 48 mice were randomly divided into six groups, which included the normal control, model control, positive control, ephedra alkaloid, ephedra polysaccharide and ephedra non-alkaloid groups. Intragastric administration of the respective treatments was provided continuously for four weeks and the body weight was recorded weekly. The total levels of cholesterol (TC), triglycerides (TG), high-density lipoprotein cholesterol (HDL-C) and malondialdehyde (MDA), and the activity levels of superoxide dismutase (SOD), alanine aminotransferase (ALT) and aspartate aminotransferase (AST) in the serum were recorded. In addition, changes in liver morphology and organ coefficients (ratio of organ to body weight) were evaluated, while the acute toxicity reactions of ephedra extractions were investigated using the modified Spearman-Karber method. Compared with the mice in the model control group, the weight, liver coefficient, serum levels of TC, TG and MDA, and activities of ALT and AST were significantly lower (P<0.05) in the mice in the ephedra non-alkaloid group. However, the level of HDL-C and the activity of SOD were markedly higher (P<0.05). Fatty degeneration of the liver in the ephedra alkaloid and non-alkaloid groups was notably improved compared with the model control group. The mean lethal dose (LD(50)) of ephedra alkaloids was 610 mg/kg, and the maximum tolerated dose of oral ephedra non-alkaloids in the mice was 367.5-fold larger than the clinical dosage in humans. In conclusion, ephedra non-alkaloids have therapeutic potential for the treatment of hyperlipidemia, since they are able to improve lipid metabolism and are relatively safe for use under the maximum tolerated dose. D.A. Spandidos 2015-02 2014-12-08 /pmc/articles/PMC4280985/ /pubmed/25574244 http://dx.doi.org/10.3892/etm.2014.2117 Text en Copyright © 2015, Spandidos Publications http://creativecommons.org/licenses/by/3.0 This is an open-access article licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported License. The article may be redistributed, reproduced, and reused for non-commercial purposes, provided the original source is properly cited. |
spellingShingle | Articles FAN, YANBO LI, JINGJING YIN, QIANG ZHANG, YISHENG XU, HUIFANG SHI, XINHUA LI, CHEN ZHOU, YAN ZHOU, CAIXIN Effect of extractions from Ephedra sinica Stapf on hyperlipidemia in mice |
title | Effect of extractions from Ephedra sinica Stapf on hyperlipidemia in mice |
title_full | Effect of extractions from Ephedra sinica Stapf on hyperlipidemia in mice |
title_fullStr | Effect of extractions from Ephedra sinica Stapf on hyperlipidemia in mice |
title_full_unstemmed | Effect of extractions from Ephedra sinica Stapf on hyperlipidemia in mice |
title_short | Effect of extractions from Ephedra sinica Stapf on hyperlipidemia in mice |
title_sort | effect of extractions from ephedra sinica stapf on hyperlipidemia in mice |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4280985/ https://www.ncbi.nlm.nih.gov/pubmed/25574244 http://dx.doi.org/10.3892/etm.2014.2117 |
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