Cargando…
The Kidney Transcriptome and Proteome Defined by Transcriptomics and Antibody-Based Profiling
To understand renal functions and disease, it is important to define the molecular constituents of the various compartments of the kidney. Here, we used comparative transcriptomic analysis of all major organs and tissues in the human body, in combination with kidney tissue micro array based immunohi...
Autores principales: | , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2014
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4281243/ https://www.ncbi.nlm.nih.gov/pubmed/25551756 http://dx.doi.org/10.1371/journal.pone.0116125 |
_version_ | 1782350969262047232 |
---|---|
author | Habuka, Masato Fagerberg, Linn Hallström, Björn M. Kampf, Caroline Edlund, Karolina Sivertsson, Åsa Yamamoto, Tadashi Pontén, Fredrik Uhlén, Mathias Odeberg, Jacob |
author_facet | Habuka, Masato Fagerberg, Linn Hallström, Björn M. Kampf, Caroline Edlund, Karolina Sivertsson, Åsa Yamamoto, Tadashi Pontén, Fredrik Uhlén, Mathias Odeberg, Jacob |
author_sort | Habuka, Masato |
collection | PubMed |
description | To understand renal functions and disease, it is important to define the molecular constituents of the various compartments of the kidney. Here, we used comparative transcriptomic analysis of all major organs and tissues in the human body, in combination with kidney tissue micro array based immunohistochemistry, to generate a comprehensive description of the kidney-specific transcriptome and proteome. A special emphasis was placed on the identification of genes and proteins that were elevated in specific kidney subcompartments. Our analysis identified close to 400 genes that had elevated expression in the kidney, as compared to the other analysed tissues, and these were further subdivided, depending on expression levels, into tissue enriched, group enriched or tissue enhanced. Immunohistochemistry allowed us to identify proteins with distinct localisation to the glomeruli (n = 11), proximal tubules (n = 120), distal tubules (n = 9) or collecting ducts (n = 8). Among the identified kidney elevated transcripts, we found several proteins not previously characterised or identified as elevated in kidney. This description of the kidney specific transcriptome and proteome provides a resource for basic and clinical research to facilitate studies to understand kidney biology and disease. |
format | Online Article Text |
id | pubmed-4281243 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-42812432015-01-07 The Kidney Transcriptome and Proteome Defined by Transcriptomics and Antibody-Based Profiling Habuka, Masato Fagerberg, Linn Hallström, Björn M. Kampf, Caroline Edlund, Karolina Sivertsson, Åsa Yamamoto, Tadashi Pontén, Fredrik Uhlén, Mathias Odeberg, Jacob PLoS One Research Article To understand renal functions and disease, it is important to define the molecular constituents of the various compartments of the kidney. Here, we used comparative transcriptomic analysis of all major organs and tissues in the human body, in combination with kidney tissue micro array based immunohistochemistry, to generate a comprehensive description of the kidney-specific transcriptome and proteome. A special emphasis was placed on the identification of genes and proteins that were elevated in specific kidney subcompartments. Our analysis identified close to 400 genes that had elevated expression in the kidney, as compared to the other analysed tissues, and these were further subdivided, depending on expression levels, into tissue enriched, group enriched or tissue enhanced. Immunohistochemistry allowed us to identify proteins with distinct localisation to the glomeruli (n = 11), proximal tubules (n = 120), distal tubules (n = 9) or collecting ducts (n = 8). Among the identified kidney elevated transcripts, we found several proteins not previously characterised or identified as elevated in kidney. This description of the kidney specific transcriptome and proteome provides a resource for basic and clinical research to facilitate studies to understand kidney biology and disease. Public Library of Science 2014-12-31 /pmc/articles/PMC4281243/ /pubmed/25551756 http://dx.doi.org/10.1371/journal.pone.0116125 Text en © 2014 Habuka et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Habuka, Masato Fagerberg, Linn Hallström, Björn M. Kampf, Caroline Edlund, Karolina Sivertsson, Åsa Yamamoto, Tadashi Pontén, Fredrik Uhlén, Mathias Odeberg, Jacob The Kidney Transcriptome and Proteome Defined by Transcriptomics and Antibody-Based Profiling |
title | The Kidney Transcriptome and Proteome Defined by Transcriptomics and Antibody-Based Profiling |
title_full | The Kidney Transcriptome and Proteome Defined by Transcriptomics and Antibody-Based Profiling |
title_fullStr | The Kidney Transcriptome and Proteome Defined by Transcriptomics and Antibody-Based Profiling |
title_full_unstemmed | The Kidney Transcriptome and Proteome Defined by Transcriptomics and Antibody-Based Profiling |
title_short | The Kidney Transcriptome and Proteome Defined by Transcriptomics and Antibody-Based Profiling |
title_sort | kidney transcriptome and proteome defined by transcriptomics and antibody-based profiling |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4281243/ https://www.ncbi.nlm.nih.gov/pubmed/25551756 http://dx.doi.org/10.1371/journal.pone.0116125 |
work_keys_str_mv | AT habukamasato thekidneytranscriptomeandproteomedefinedbytranscriptomicsandantibodybasedprofiling AT fagerberglinn thekidneytranscriptomeandproteomedefinedbytranscriptomicsandantibodybasedprofiling AT hallstrombjornm thekidneytranscriptomeandproteomedefinedbytranscriptomicsandantibodybasedprofiling AT kampfcaroline thekidneytranscriptomeandproteomedefinedbytranscriptomicsandantibodybasedprofiling AT edlundkarolina thekidneytranscriptomeandproteomedefinedbytranscriptomicsandantibodybasedprofiling AT sivertssonasa thekidneytranscriptomeandproteomedefinedbytranscriptomicsandantibodybasedprofiling AT yamamototadashi thekidneytranscriptomeandproteomedefinedbytranscriptomicsandantibodybasedprofiling AT pontenfredrik thekidneytranscriptomeandproteomedefinedbytranscriptomicsandantibodybasedprofiling AT uhlenmathias thekidneytranscriptomeandproteomedefinedbytranscriptomicsandantibodybasedprofiling AT odebergjacob thekidneytranscriptomeandproteomedefinedbytranscriptomicsandantibodybasedprofiling AT habukamasato kidneytranscriptomeandproteomedefinedbytranscriptomicsandantibodybasedprofiling AT fagerberglinn kidneytranscriptomeandproteomedefinedbytranscriptomicsandantibodybasedprofiling AT hallstrombjornm kidneytranscriptomeandproteomedefinedbytranscriptomicsandantibodybasedprofiling AT kampfcaroline kidneytranscriptomeandproteomedefinedbytranscriptomicsandantibodybasedprofiling AT edlundkarolina kidneytranscriptomeandproteomedefinedbytranscriptomicsandantibodybasedprofiling AT sivertssonasa kidneytranscriptomeandproteomedefinedbytranscriptomicsandantibodybasedprofiling AT yamamototadashi kidneytranscriptomeandproteomedefinedbytranscriptomicsandantibodybasedprofiling AT pontenfredrik kidneytranscriptomeandproteomedefinedbytranscriptomicsandantibodybasedprofiling AT uhlenmathias kidneytranscriptomeandproteomedefinedbytranscriptomicsandantibodybasedprofiling AT odebergjacob kidneytranscriptomeandproteomedefinedbytranscriptomicsandantibodybasedprofiling |