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Coptis chinensis and Myrobalan (Terminalia chebula) Can Synergistically Inhibit Inflammatory Response In Vitro and In Vivo

Objectives. To investigate the anti-inflammatory effect of Coptis chinensis plus myrobalan (CM) in vitro and in vivo. Methods. The inflammation in mouse peritoneal macrophages was induced by lipopolysaccharide (LPS). Animal models were established by using ear swelling and paw edema of mouse induced...

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Autores principales: Cui, Enhui, Zhi, Xiaoyan, Chen, Ying, Gao, Yuanyuan, Fan, Yunpeng, Zhang, Weimin, Ma, Wuren, Hou, Weifeng, Guo, Chao, Song, Xiaoping
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4281439/
https://www.ncbi.nlm.nih.gov/pubmed/25587343
http://dx.doi.org/10.1155/2014/510157
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author Cui, Enhui
Zhi, Xiaoyan
Chen, Ying
Gao, Yuanyuan
Fan, Yunpeng
Zhang, Weimin
Ma, Wuren
Hou, Weifeng
Guo, Chao
Song, Xiaoping
author_facet Cui, Enhui
Zhi, Xiaoyan
Chen, Ying
Gao, Yuanyuan
Fan, Yunpeng
Zhang, Weimin
Ma, Wuren
Hou, Weifeng
Guo, Chao
Song, Xiaoping
author_sort Cui, Enhui
collection PubMed
description Objectives. To investigate the anti-inflammatory effect of Coptis chinensis plus myrobalan (CM) in vitro and in vivo. Methods. The inflammation in mouse peritoneal macrophages was induced by lipopolysaccharide (LPS). Animal models were established by using ear swelling and paw edema of mouse induced by xylene and formaldehyde, respectively. In vitro, cytotoxicity, the phagocytosis of macrophages, the levels of nitric oxide (NO), induced nitric oxide synthase (iNOS), tumor necrosis factor-α (TNF-α), and interleukin-6 (IL-6) in cell supernatant were detected. In vivo, swelling rate and edema inhibitory rate of ear and paw were observed using CM-treated mice. Results. At 150–18.75 μg·mL(−1), CM had no cytotoxicity and could significantly promote the growth and the phagocytosis of macrophages and inhibit the overproduction of NO, iNOS, TNF-α, and IL-6 in macrophages induced by LPS. In vivo, pretreatment with CM, the ear swelling, and paw edema of mice could be significantly inhibited in a dose-dependent manner, and the antiedema effect of CM at high dose was better than dexamethasone. Conclusion. Our results demonstrated that Coptis chinensis and myrobalan possessed synergistically anti-inflammatory activities in vitro and in vivo, which indicated that CM had therapeutic potential for the prevention and treatment of inflammation-mediated diseases.
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spelling pubmed-42814392015-01-13 Coptis chinensis and Myrobalan (Terminalia chebula) Can Synergistically Inhibit Inflammatory Response In Vitro and In Vivo Cui, Enhui Zhi, Xiaoyan Chen, Ying Gao, Yuanyuan Fan, Yunpeng Zhang, Weimin Ma, Wuren Hou, Weifeng Guo, Chao Song, Xiaoping Evid Based Complement Alternat Med Research Article Objectives. To investigate the anti-inflammatory effect of Coptis chinensis plus myrobalan (CM) in vitro and in vivo. Methods. The inflammation in mouse peritoneal macrophages was induced by lipopolysaccharide (LPS). Animal models were established by using ear swelling and paw edema of mouse induced by xylene and formaldehyde, respectively. In vitro, cytotoxicity, the phagocytosis of macrophages, the levels of nitric oxide (NO), induced nitric oxide synthase (iNOS), tumor necrosis factor-α (TNF-α), and interleukin-6 (IL-6) in cell supernatant were detected. In vivo, swelling rate and edema inhibitory rate of ear and paw were observed using CM-treated mice. Results. At 150–18.75 μg·mL(−1), CM had no cytotoxicity and could significantly promote the growth and the phagocytosis of macrophages and inhibit the overproduction of NO, iNOS, TNF-α, and IL-6 in macrophages induced by LPS. In vivo, pretreatment with CM, the ear swelling, and paw edema of mice could be significantly inhibited in a dose-dependent manner, and the antiedema effect of CM at high dose was better than dexamethasone. Conclusion. Our results demonstrated that Coptis chinensis and myrobalan possessed synergistically anti-inflammatory activities in vitro and in vivo, which indicated that CM had therapeutic potential for the prevention and treatment of inflammation-mediated diseases. Hindawi Publishing Corporation 2014 2014-12-18 /pmc/articles/PMC4281439/ /pubmed/25587343 http://dx.doi.org/10.1155/2014/510157 Text en Copyright © 2014 Enhui Cui et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Cui, Enhui
Zhi, Xiaoyan
Chen, Ying
Gao, Yuanyuan
Fan, Yunpeng
Zhang, Weimin
Ma, Wuren
Hou, Weifeng
Guo, Chao
Song, Xiaoping
Coptis chinensis and Myrobalan (Terminalia chebula) Can Synergistically Inhibit Inflammatory Response In Vitro and In Vivo
title Coptis chinensis and Myrobalan (Terminalia chebula) Can Synergistically Inhibit Inflammatory Response In Vitro and In Vivo
title_full Coptis chinensis and Myrobalan (Terminalia chebula) Can Synergistically Inhibit Inflammatory Response In Vitro and In Vivo
title_fullStr Coptis chinensis and Myrobalan (Terminalia chebula) Can Synergistically Inhibit Inflammatory Response In Vitro and In Vivo
title_full_unstemmed Coptis chinensis and Myrobalan (Terminalia chebula) Can Synergistically Inhibit Inflammatory Response In Vitro and In Vivo
title_short Coptis chinensis and Myrobalan (Terminalia chebula) Can Synergistically Inhibit Inflammatory Response In Vitro and In Vivo
title_sort coptis chinensis and myrobalan (terminalia chebula) can synergistically inhibit inflammatory response in vitro and in vivo
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4281439/
https://www.ncbi.nlm.nih.gov/pubmed/25587343
http://dx.doi.org/10.1155/2014/510157
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