Cargando…
Chemical Assessment and Antimicrobial and Antioxidant Activities of Endophytic Fungi Extracts Isolated from Costus spiralis (Jacq.) Roscoe (Costaceae)
Costus spiralis (Costaceae) is a species native to the Amazon region and is used in traditional medicine. The endophytic fungi used in this study were obtained from leaves of this plant. 13 strains were selected to obtain hydroethanolic extracts and were submitted to hydroalcoholic extraction and ev...
Autores principales: | , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi Publishing Corporation
2014
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4281452/ https://www.ncbi.nlm.nih.gov/pubmed/25587339 http://dx.doi.org/10.1155/2014/190543 |
_version_ | 1782351003225423872 |
---|---|
author | Marson Ascêncio, Poliana Guerino Ascêncio, Sérgio Donizeti Aguiar, Aline Aires Fiorini, Adriana Pimenta, Raphael Sanzio |
author_facet | Marson Ascêncio, Poliana Guerino Ascêncio, Sérgio Donizeti Aguiar, Aline Aires Fiorini, Adriana Pimenta, Raphael Sanzio |
author_sort | Marson Ascêncio, Poliana Guerino |
collection | PubMed |
description | Costus spiralis (Costaceae) is a species native to the Amazon region and is used in traditional medicine. The endophytic fungi used in this study were obtained from leaves of this plant. 13 strains were selected to obtain hydroethanolic extracts and were submitted to hydroalcoholic extraction and evaluated for antioxidant activity by DPPH (2,2-difenil-1-picrilhidrazil) and FRAP (ferric reducing antioxidant power), and all of the fungi had positive results. The antimicrobial action of crude extracts had a good range of activities. All extracts had inhibitory activities against the yeasts of Candida albicans and C. parapsilosis, with 125 to 500 μg/mL MIC. Eight extracts had antimicrobial activities against Bacillus subtilis (MIC from 62.4 to 125 μg/mL), 5 against Pseudomonas aeruginosa (MIC from 125 to 500 μg/mL), 2 against Salmonella enterica (MIC from 125 to 62.5 μg/mL), and 2 against Enterococcus faecalis (MIC from 500 to 125 μg/mL). The presence of secondary metabolites, including coumarins, was observed during chemical evaluation by thin layer chromatography. Total phenol content was estimated, and a strong positive correlation to antioxidant activity was observed, according to its Pearson coefficient. This is the first report of the bioactive potential of endophytic fungi isolated from the Costaceae family in Brazilian ecosystems. |
format | Online Article Text |
id | pubmed-4281452 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | Hindawi Publishing Corporation |
record_format | MEDLINE/PubMed |
spelling | pubmed-42814522015-01-13 Chemical Assessment and Antimicrobial and Antioxidant Activities of Endophytic Fungi Extracts Isolated from Costus spiralis (Jacq.) Roscoe (Costaceae) Marson Ascêncio, Poliana Guerino Ascêncio, Sérgio Donizeti Aguiar, Aline Aires Fiorini, Adriana Pimenta, Raphael Sanzio Evid Based Complement Alternat Med Research Article Costus spiralis (Costaceae) is a species native to the Amazon region and is used in traditional medicine. The endophytic fungi used in this study were obtained from leaves of this plant. 13 strains were selected to obtain hydroethanolic extracts and were submitted to hydroalcoholic extraction and evaluated for antioxidant activity by DPPH (2,2-difenil-1-picrilhidrazil) and FRAP (ferric reducing antioxidant power), and all of the fungi had positive results. The antimicrobial action of crude extracts had a good range of activities. All extracts had inhibitory activities against the yeasts of Candida albicans and C. parapsilosis, with 125 to 500 μg/mL MIC. Eight extracts had antimicrobial activities against Bacillus subtilis (MIC from 62.4 to 125 μg/mL), 5 against Pseudomonas aeruginosa (MIC from 125 to 500 μg/mL), 2 against Salmonella enterica (MIC from 125 to 62.5 μg/mL), and 2 against Enterococcus faecalis (MIC from 500 to 125 μg/mL). The presence of secondary metabolites, including coumarins, was observed during chemical evaluation by thin layer chromatography. Total phenol content was estimated, and a strong positive correlation to antioxidant activity was observed, according to its Pearson coefficient. This is the first report of the bioactive potential of endophytic fungi isolated from the Costaceae family in Brazilian ecosystems. Hindawi Publishing Corporation 2014 2014-12-17 /pmc/articles/PMC4281452/ /pubmed/25587339 http://dx.doi.org/10.1155/2014/190543 Text en Copyright © 2014 Poliana Guerino Marson Ascêncio et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Marson Ascêncio, Poliana Guerino Ascêncio, Sérgio Donizeti Aguiar, Aline Aires Fiorini, Adriana Pimenta, Raphael Sanzio Chemical Assessment and Antimicrobial and Antioxidant Activities of Endophytic Fungi Extracts Isolated from Costus spiralis (Jacq.) Roscoe (Costaceae) |
title | Chemical Assessment and Antimicrobial and Antioxidant Activities of Endophytic Fungi Extracts Isolated from Costus spiralis (Jacq.) Roscoe (Costaceae) |
title_full | Chemical Assessment and Antimicrobial and Antioxidant Activities of Endophytic Fungi Extracts Isolated from Costus spiralis (Jacq.) Roscoe (Costaceae) |
title_fullStr | Chemical Assessment and Antimicrobial and Antioxidant Activities of Endophytic Fungi Extracts Isolated from Costus spiralis (Jacq.) Roscoe (Costaceae) |
title_full_unstemmed | Chemical Assessment and Antimicrobial and Antioxidant Activities of Endophytic Fungi Extracts Isolated from Costus spiralis (Jacq.) Roscoe (Costaceae) |
title_short | Chemical Assessment and Antimicrobial and Antioxidant Activities of Endophytic Fungi Extracts Isolated from Costus spiralis (Jacq.) Roscoe (Costaceae) |
title_sort | chemical assessment and antimicrobial and antioxidant activities of endophytic fungi extracts isolated from costus spiralis (jacq.) roscoe (costaceae) |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4281452/ https://www.ncbi.nlm.nih.gov/pubmed/25587339 http://dx.doi.org/10.1155/2014/190543 |
work_keys_str_mv | AT marsonascenciopolianaguerino chemicalassessmentandantimicrobialandantioxidantactivitiesofendophyticfungiextractsisolatedfromcostusspiralisjacqroscoecostaceae AT ascenciosergiodonizeti chemicalassessmentandantimicrobialandantioxidantactivitiesofendophyticfungiextractsisolatedfromcostusspiralisjacqroscoecostaceae AT aguiaralineaires chemicalassessmentandantimicrobialandantioxidantactivitiesofendophyticfungiextractsisolatedfromcostusspiralisjacqroscoecostaceae AT fioriniadriana chemicalassessmentandantimicrobialandantioxidantactivitiesofendophyticfungiextractsisolatedfromcostusspiralisjacqroscoecostaceae AT pimentaraphaelsanzio chemicalassessmentandantimicrobialandantioxidantactivitiesofendophyticfungiextractsisolatedfromcostusspiralisjacqroscoecostaceae |