Cargando…

Chemical Assessment and Antimicrobial and Antioxidant Activities of Endophytic Fungi Extracts Isolated from Costus spiralis (Jacq.) Roscoe (Costaceae)

Costus spiralis (Costaceae) is a species native to the Amazon region and is used in traditional medicine. The endophytic fungi used in this study were obtained from leaves of this plant. 13 strains were selected to obtain hydroethanolic extracts and were submitted to hydroalcoholic extraction and ev...

Descripción completa

Detalles Bibliográficos
Autores principales: Marson Ascêncio, Poliana Guerino, Ascêncio, Sérgio Donizeti, Aguiar, Aline Aires, Fiorini, Adriana, Pimenta, Raphael Sanzio
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4281452/
https://www.ncbi.nlm.nih.gov/pubmed/25587339
http://dx.doi.org/10.1155/2014/190543
_version_ 1782351003225423872
author Marson Ascêncio, Poliana Guerino
Ascêncio, Sérgio Donizeti
Aguiar, Aline Aires
Fiorini, Adriana
Pimenta, Raphael Sanzio
author_facet Marson Ascêncio, Poliana Guerino
Ascêncio, Sérgio Donizeti
Aguiar, Aline Aires
Fiorini, Adriana
Pimenta, Raphael Sanzio
author_sort Marson Ascêncio, Poliana Guerino
collection PubMed
description Costus spiralis (Costaceae) is a species native to the Amazon region and is used in traditional medicine. The endophytic fungi used in this study were obtained from leaves of this plant. 13 strains were selected to obtain hydroethanolic extracts and were submitted to hydroalcoholic extraction and evaluated for antioxidant activity by DPPH (2,2-difenil-1-picrilhidrazil) and FRAP (ferric reducing antioxidant power), and all of the fungi had positive results. The antimicrobial action of crude extracts had a good range of activities. All extracts had inhibitory activities against the yeasts of Candida albicans and C. parapsilosis, with 125 to 500 μg/mL MIC. Eight extracts had antimicrobial activities against Bacillus subtilis (MIC from 62.4 to 125 μg/mL), 5 against Pseudomonas aeruginosa (MIC from 125 to 500 μg/mL), 2 against Salmonella enterica (MIC from 125 to 62.5 μg/mL), and 2 against Enterococcus faecalis (MIC from 500 to 125 μg/mL). The presence of secondary metabolites, including coumarins, was observed during chemical evaluation by thin layer chromatography. Total phenol content was estimated, and a strong positive correlation to antioxidant activity was observed, according to its Pearson coefficient. This is the first report of the bioactive potential of endophytic fungi isolated from the Costaceae family in Brazilian ecosystems.
format Online
Article
Text
id pubmed-4281452
institution National Center for Biotechnology Information
language English
publishDate 2014
publisher Hindawi Publishing Corporation
record_format MEDLINE/PubMed
spelling pubmed-42814522015-01-13 Chemical Assessment and Antimicrobial and Antioxidant Activities of Endophytic Fungi Extracts Isolated from Costus spiralis (Jacq.) Roscoe (Costaceae) Marson Ascêncio, Poliana Guerino Ascêncio, Sérgio Donizeti Aguiar, Aline Aires Fiorini, Adriana Pimenta, Raphael Sanzio Evid Based Complement Alternat Med Research Article Costus spiralis (Costaceae) is a species native to the Amazon region and is used in traditional medicine. The endophytic fungi used in this study were obtained from leaves of this plant. 13 strains were selected to obtain hydroethanolic extracts and were submitted to hydroalcoholic extraction and evaluated for antioxidant activity by DPPH (2,2-difenil-1-picrilhidrazil) and FRAP (ferric reducing antioxidant power), and all of the fungi had positive results. The antimicrobial action of crude extracts had a good range of activities. All extracts had inhibitory activities against the yeasts of Candida albicans and C. parapsilosis, with 125 to 500 μg/mL MIC. Eight extracts had antimicrobial activities against Bacillus subtilis (MIC from 62.4 to 125 μg/mL), 5 against Pseudomonas aeruginosa (MIC from 125 to 500 μg/mL), 2 against Salmonella enterica (MIC from 125 to 62.5 μg/mL), and 2 against Enterococcus faecalis (MIC from 500 to 125 μg/mL). The presence of secondary metabolites, including coumarins, was observed during chemical evaluation by thin layer chromatography. Total phenol content was estimated, and a strong positive correlation to antioxidant activity was observed, according to its Pearson coefficient. This is the first report of the bioactive potential of endophytic fungi isolated from the Costaceae family in Brazilian ecosystems. Hindawi Publishing Corporation 2014 2014-12-17 /pmc/articles/PMC4281452/ /pubmed/25587339 http://dx.doi.org/10.1155/2014/190543 Text en Copyright © 2014 Poliana Guerino Marson Ascêncio et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Marson Ascêncio, Poliana Guerino
Ascêncio, Sérgio Donizeti
Aguiar, Aline Aires
Fiorini, Adriana
Pimenta, Raphael Sanzio
Chemical Assessment and Antimicrobial and Antioxidant Activities of Endophytic Fungi Extracts Isolated from Costus spiralis (Jacq.) Roscoe (Costaceae)
title Chemical Assessment and Antimicrobial and Antioxidant Activities of Endophytic Fungi Extracts Isolated from Costus spiralis (Jacq.) Roscoe (Costaceae)
title_full Chemical Assessment and Antimicrobial and Antioxidant Activities of Endophytic Fungi Extracts Isolated from Costus spiralis (Jacq.) Roscoe (Costaceae)
title_fullStr Chemical Assessment and Antimicrobial and Antioxidant Activities of Endophytic Fungi Extracts Isolated from Costus spiralis (Jacq.) Roscoe (Costaceae)
title_full_unstemmed Chemical Assessment and Antimicrobial and Antioxidant Activities of Endophytic Fungi Extracts Isolated from Costus spiralis (Jacq.) Roscoe (Costaceae)
title_short Chemical Assessment and Antimicrobial and Antioxidant Activities of Endophytic Fungi Extracts Isolated from Costus spiralis (Jacq.) Roscoe (Costaceae)
title_sort chemical assessment and antimicrobial and antioxidant activities of endophytic fungi extracts isolated from costus spiralis (jacq.) roscoe (costaceae)
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4281452/
https://www.ncbi.nlm.nih.gov/pubmed/25587339
http://dx.doi.org/10.1155/2014/190543
work_keys_str_mv AT marsonascenciopolianaguerino chemicalassessmentandantimicrobialandantioxidantactivitiesofendophyticfungiextractsisolatedfromcostusspiralisjacqroscoecostaceae
AT ascenciosergiodonizeti chemicalassessmentandantimicrobialandantioxidantactivitiesofendophyticfungiextractsisolatedfromcostusspiralisjacqroscoecostaceae
AT aguiaralineaires chemicalassessmentandantimicrobialandantioxidantactivitiesofendophyticfungiextractsisolatedfromcostusspiralisjacqroscoecostaceae
AT fioriniadriana chemicalassessmentandantimicrobialandantioxidantactivitiesofendophyticfungiextractsisolatedfromcostusspiralisjacqroscoecostaceae
AT pimentaraphaelsanzio chemicalassessmentandantimicrobialandantioxidantactivitiesofendophyticfungiextractsisolatedfromcostusspiralisjacqroscoecostaceae