Cargando…

Characterization of Antibiotic Producing Rare Actinomycete Nonomuraea sp. JAJ18 Derived from an Indian Coastal Solar Saltern

Rare actinomycete genera are accepted as a promising source of novel metabolites having pharmaceutical importance. One such genus of rare actinomycete is Nonomuraea. The present study was aimed at characterizing the antibiotic producing Nonomuraea strain JAJ18 which was previously isolated from coas...

Descripción completa

Detalles Bibliográficos
Autores principales: Arul Jose, Polpass, Sivakala, Kunjukrishnan Kamalakshi, Rajeswari, Pandiyan, Jebakumar, Solomon Robinson David
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4281464/
https://www.ncbi.nlm.nih.gov/pubmed/25587565
http://dx.doi.org/10.1155/2014/456070
_version_ 1782351005783949312
author Arul Jose, Polpass
Sivakala, Kunjukrishnan Kamalakshi
Rajeswari, Pandiyan
Jebakumar, Solomon Robinson David
author_facet Arul Jose, Polpass
Sivakala, Kunjukrishnan Kamalakshi
Rajeswari, Pandiyan
Jebakumar, Solomon Robinson David
author_sort Arul Jose, Polpass
collection PubMed
description Rare actinomycete genera are accepted as a promising source of novel metabolites having pharmaceutical importance. One such genus of rare actinomycete is Nonomuraea. The present study was aimed at characterizing the antibiotic producing Nonomuraea strain JAJ18 which was previously isolated from coastal solar saltern. Strain JAJ18 was recognized as a member of genus Nonomuraea based on its almost complete 16S rRNA gene sequence and phenotypic characteristics. The strain JAJ18 was found to be closely related to Nonomuraea maheshkhaliensis 16-5-14(T) (98.90%), Nonomuraea candida HMC10(T) (98.58%), and Nonomuraea jabiensis A4036(T) (98.43%). From cell-free culture broth of strain JAJ18, an antibiotic was extracted and purified by silica column chromatography. The obtained antibiotic was found to be active against a range of Gram-positive and Gram-negative bacteria including drug-resistant Staphylococcus, with minimal inhibitory concentration (MIC) ranging from 0.5 to 16.0 µg mL(−1). The structural characteristics of antibiotic were determined by FTIR and NMR spectroscopy. The antibiotic was identified to be an aliphatic rich compound with significant dissimilarity to known antibiotics reported from members of the genus, Nonomuraea. As the trends to discover novel metabolites from Nonomuraea are vibrant, further studies are needed to understand the structural and biotechnological significance of antibiotic compound produced by Nonomuraea sp. JAJ18.
format Online
Article
Text
id pubmed-4281464
institution National Center for Biotechnology Information
language English
publishDate 2014
publisher Hindawi Publishing Corporation
record_format MEDLINE/PubMed
spelling pubmed-42814642015-01-13 Characterization of Antibiotic Producing Rare Actinomycete Nonomuraea sp. JAJ18 Derived from an Indian Coastal Solar Saltern Arul Jose, Polpass Sivakala, Kunjukrishnan Kamalakshi Rajeswari, Pandiyan Jebakumar, Solomon Robinson David ScientificWorldJournal Research Article Rare actinomycete genera are accepted as a promising source of novel metabolites having pharmaceutical importance. One such genus of rare actinomycete is Nonomuraea. The present study was aimed at characterizing the antibiotic producing Nonomuraea strain JAJ18 which was previously isolated from coastal solar saltern. Strain JAJ18 was recognized as a member of genus Nonomuraea based on its almost complete 16S rRNA gene sequence and phenotypic characteristics. The strain JAJ18 was found to be closely related to Nonomuraea maheshkhaliensis 16-5-14(T) (98.90%), Nonomuraea candida HMC10(T) (98.58%), and Nonomuraea jabiensis A4036(T) (98.43%). From cell-free culture broth of strain JAJ18, an antibiotic was extracted and purified by silica column chromatography. The obtained antibiotic was found to be active against a range of Gram-positive and Gram-negative bacteria including drug-resistant Staphylococcus, with minimal inhibitory concentration (MIC) ranging from 0.5 to 16.0 µg mL(−1). The structural characteristics of antibiotic were determined by FTIR and NMR spectroscopy. The antibiotic was identified to be an aliphatic rich compound with significant dissimilarity to known antibiotics reported from members of the genus, Nonomuraea. As the trends to discover novel metabolites from Nonomuraea are vibrant, further studies are needed to understand the structural and biotechnological significance of antibiotic compound produced by Nonomuraea sp. JAJ18. Hindawi Publishing Corporation 2014 2014-12-18 /pmc/articles/PMC4281464/ /pubmed/25587565 http://dx.doi.org/10.1155/2014/456070 Text en Copyright © 2014 Polpass Arul Jose et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Arul Jose, Polpass
Sivakala, Kunjukrishnan Kamalakshi
Rajeswari, Pandiyan
Jebakumar, Solomon Robinson David
Characterization of Antibiotic Producing Rare Actinomycete Nonomuraea sp. JAJ18 Derived from an Indian Coastal Solar Saltern
title Characterization of Antibiotic Producing Rare Actinomycete Nonomuraea sp. JAJ18 Derived from an Indian Coastal Solar Saltern
title_full Characterization of Antibiotic Producing Rare Actinomycete Nonomuraea sp. JAJ18 Derived from an Indian Coastal Solar Saltern
title_fullStr Characterization of Antibiotic Producing Rare Actinomycete Nonomuraea sp. JAJ18 Derived from an Indian Coastal Solar Saltern
title_full_unstemmed Characterization of Antibiotic Producing Rare Actinomycete Nonomuraea sp. JAJ18 Derived from an Indian Coastal Solar Saltern
title_short Characterization of Antibiotic Producing Rare Actinomycete Nonomuraea sp. JAJ18 Derived from an Indian Coastal Solar Saltern
title_sort characterization of antibiotic producing rare actinomycete nonomuraea sp. jaj18 derived from an indian coastal solar saltern
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4281464/
https://www.ncbi.nlm.nih.gov/pubmed/25587565
http://dx.doi.org/10.1155/2014/456070
work_keys_str_mv AT aruljosepolpass characterizationofantibioticproducingrareactinomycetenonomuraeaspjaj18derivedfromanindiancoastalsolarsaltern
AT sivakalakunjukrishnankamalakshi characterizationofantibioticproducingrareactinomycetenonomuraeaspjaj18derivedfromanindiancoastalsolarsaltern
AT rajeswaripandiyan characterizationofantibioticproducingrareactinomycetenonomuraeaspjaj18derivedfromanindiancoastalsolarsaltern
AT jebakumarsolomonrobinsondavid characterizationofantibioticproducingrareactinomycetenonomuraeaspjaj18derivedfromanindiancoastalsolarsaltern