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Effects of temperature and glycerol and methanol-feeding profiles on the production of recombinant galactose oxidase in Pichia pastoris

Optimization of protein production from methanol-induced Pichia pastoris cultures is necessary to ensure high productivity rates and high yields of recombinant proteins. We investigated the effects of temperature and different linear or exponential methanol-feeding rates on the production of recombi...

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Detalles Bibliográficos
Autores principales:	Anasontzis, George E, Salazar Penã, Margarita, Spadiut, Oliver, Brumer, Harry, Olsson, Lisbeth
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	BlackWell Publishing Ltd 2014
Materias:	Cell Culture and Tissue Engineering
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4282061/ https://www.ncbi.nlm.nih.gov/pubmed/24493559 http://dx.doi.org/10.1002/btpr.1878

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