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Induction of Primary Male in Juvenile Red Spotted Grouper Epinephelus akaara by Immersion of 17α-Methyltestosterone
We investigated the androgenic effects of 17α- methyltestosterone (MT) on gonadal sex reversal in juvenile red spotted grouper Epinephelus akaara. The fish were immersed in 17α-MT at 1 and 5 mg/L. Treatment method of 17α-MT was once weekly for 4 and 8 weeks. Fish were sampled at 12 months after end...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Korean Society of Developmental Biology
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4282206/ https://www.ncbi.nlm.nih.gov/pubmed/25949180 http://dx.doi.org/10.12717/DR.2014.18.3.127 |
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author | Lee, Chi-Hoon Hur, Sang-Woo Na, Oh-Soo Baek, Hae-Ja Noh, Choong-Hwan Han, Sang-Hyun Lee, Young-Don |
author_facet | Lee, Chi-Hoon Hur, Sang-Woo Na, Oh-Soo Baek, Hae-Ja Noh, Choong-Hwan Han, Sang-Hyun Lee, Young-Don |
author_sort | Lee, Chi-Hoon |
collection | PubMed |
description | We investigated the androgenic effects of 17α- methyltestosterone (MT) on gonadal sex reversal in juvenile red spotted grouper Epinephelus akaara. The fish were immersed in 17α-MT at 1 and 5 mg/L. Treatment method of 17α-MT was once weekly for 4 and 8 weeks. Fish were sampled at 12 months after end of the treatment period in order to histological analysis. At the initiation of an experiment (70 day after hatching), juvenile red spotted grouper have the paired primordial gonads with somatic cells bellow kidney in the posterior portion of the body cavity. Formation of ovarian cavity indicates that the ovarian differentiation beginning at 70 DAH in red spotted grouper. At 12 months after end of the treatment period, control group, 17α-MT 1 mg/L treatment group for 4 and 8 weeks, and 17α-MT 5 mg/L treatment group for 4 weeks were all female. However, sex-changed males without ovarian cavity were observed in the 17α-MT 5 mg/L treatment group for 8 weeks. In grouper, we firstly reported that the red spotted grouper be able to induce the primary males by hormone treatment prior to gonadal sex differentiation. |
format | Online Article Text |
id | pubmed-4282206 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | Korean Society of Developmental Biology |
record_format | MEDLINE/PubMed |
spelling | pubmed-42822062015-05-06 Induction of Primary Male in Juvenile Red Spotted Grouper Epinephelus akaara by Immersion of 17α-Methyltestosterone Lee, Chi-Hoon Hur, Sang-Woo Na, Oh-Soo Baek, Hae-Ja Noh, Choong-Hwan Han, Sang-Hyun Lee, Young-Don Dev Reprod Article We investigated the androgenic effects of 17α- methyltestosterone (MT) on gonadal sex reversal in juvenile red spotted grouper Epinephelus akaara. The fish were immersed in 17α-MT at 1 and 5 mg/L. Treatment method of 17α-MT was once weekly for 4 and 8 weeks. Fish were sampled at 12 months after end of the treatment period in order to histological analysis. At the initiation of an experiment (70 day after hatching), juvenile red spotted grouper have the paired primordial gonads with somatic cells bellow kidney in the posterior portion of the body cavity. Formation of ovarian cavity indicates that the ovarian differentiation beginning at 70 DAH in red spotted grouper. At 12 months after end of the treatment period, control group, 17α-MT 1 mg/L treatment group for 4 and 8 weeks, and 17α-MT 5 mg/L treatment group for 4 weeks were all female. However, sex-changed males without ovarian cavity were observed in the 17α-MT 5 mg/L treatment group for 8 weeks. In grouper, we firstly reported that the red spotted grouper be able to induce the primary males by hormone treatment prior to gonadal sex differentiation. Korean Society of Developmental Biology 2014-09 /pmc/articles/PMC4282206/ /pubmed/25949180 http://dx.doi.org/10.12717/DR.2014.18.3.127 Text en © Korean Society of Developmental Biology. All Rights Reserved |
spellingShingle | Article Lee, Chi-Hoon Hur, Sang-Woo Na, Oh-Soo Baek, Hae-Ja Noh, Choong-Hwan Han, Sang-Hyun Lee, Young-Don Induction of Primary Male in Juvenile Red Spotted Grouper Epinephelus akaara by Immersion of 17α-Methyltestosterone |
title | Induction of Primary Male in Juvenile Red Spotted Grouper Epinephelus akaara by Immersion of 17α-Methyltestosterone |
title_full | Induction of Primary Male in Juvenile Red Spotted Grouper Epinephelus akaara by Immersion of 17α-Methyltestosterone |
title_fullStr | Induction of Primary Male in Juvenile Red Spotted Grouper Epinephelus akaara by Immersion of 17α-Methyltestosterone |
title_full_unstemmed | Induction of Primary Male in Juvenile Red Spotted Grouper Epinephelus akaara by Immersion of 17α-Methyltestosterone |
title_short | Induction of Primary Male in Juvenile Red Spotted Grouper Epinephelus akaara by Immersion of 17α-Methyltestosterone |
title_sort | induction of primary male in juvenile red spotted grouper epinephelus akaara by immersion of 17α-methyltestosterone |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4282206/ https://www.ncbi.nlm.nih.gov/pubmed/25949180 http://dx.doi.org/10.12717/DR.2014.18.3.127 |
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