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Characterization of Pubertal Development Phases in Female Longtooth Grouper, Epinephelus bruneus via Classification of Bodyweight

Puberty is the developmental period which animals obtain the ability of reproducing sexually for the first time in life. In commercially important aquaculture fish species, the onset of puberty is a matter of major interest due to controlling of sexual maturation to improve broodstock management. To...

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Autores principales: Ryu, Yong-Woon, Hur, Sang-Woo, Hur, Sung-Pyo, Lee, Chi-Hoon, Lim, Bong-Soo, Lee, Young-Don
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Korean Society of Developmental Biology 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4282214/
https://www.ncbi.nlm.nih.gov/pubmed/25949121
http://dx.doi.org/10.12717/DR.2013.17.1.055
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author Ryu, Yong-Woon
Hur, Sang-Woo
Hur, Sung-Pyo
Lee, Chi-Hoon
Lim, Bong-Soo
Lee, Young-Don
author_facet Ryu, Yong-Woon
Hur, Sang-Woo
Hur, Sung-Pyo
Lee, Chi-Hoon
Lim, Bong-Soo
Lee, Young-Don
author_sort Ryu, Yong-Woon
collection PubMed
description Puberty is the developmental period which animals obtain the ability of reproducing sexually for the first time in life. In commercially important aquaculture fish species, the onset of puberty is a matter of major interest due to controlling of sexual maturation to improve broodstock management. To investigate pubertal characteristics of female longtooth grouper (Epinephelus bruneus), specimens were classified into three groups by the bodyweight, including 1, 2, and 3 kg group. Thereafter, we focused on ovarian development and level changes of endocrine regulation factors (GnRH, GTHs, steroid hormone). In the non-breeding season (April), the levels of endocrine regulation factors showed increasing trends in accordance with bodyweight gaining; nevertheless, the oocytes were growth phase belongs to almost peri-nucleous stages in all groups. In the breeding season (June), the levels of endocrine regulation factors were fluctuated that decreases in levels of sbGnRH and FSHβ mRNA expressions along with serum E(2) concentrations in 3 kg of group. However, LHβ mRNA expression levels sustained increasing trends by the bodyweight. Moreover, the oocytes developed that 2 kg and 3 kg groups obtained plentiful vitellogenic oocytes while 1 kg group was still composed with greater part of pre-vitellogenic oocytes. Especially, the oocytes of 3 kg group reached over 450 μm of diameters that indicating possibility to enter the final maturations. These results suggest that the progress of pubertal development in female E. bruneus could be classify into three phases via bodyweight, including pre-puberty (1 kg), early-puberty (2 kg) and puberty (3 kg).
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spelling pubmed-42822142015-05-06 Characterization of Pubertal Development Phases in Female Longtooth Grouper, Epinephelus bruneus via Classification of Bodyweight Ryu, Yong-Woon Hur, Sang-Woo Hur, Sung-Pyo Lee, Chi-Hoon Lim, Bong-Soo Lee, Young-Don Dev Reprod Article Puberty is the developmental period which animals obtain the ability of reproducing sexually for the first time in life. In commercially important aquaculture fish species, the onset of puberty is a matter of major interest due to controlling of sexual maturation to improve broodstock management. To investigate pubertal characteristics of female longtooth grouper (Epinephelus bruneus), specimens were classified into three groups by the bodyweight, including 1, 2, and 3 kg group. Thereafter, we focused on ovarian development and level changes of endocrine regulation factors (GnRH, GTHs, steroid hormone). In the non-breeding season (April), the levels of endocrine regulation factors showed increasing trends in accordance with bodyweight gaining; nevertheless, the oocytes were growth phase belongs to almost peri-nucleous stages in all groups. In the breeding season (June), the levels of endocrine regulation factors were fluctuated that decreases in levels of sbGnRH and FSHβ mRNA expressions along with serum E(2) concentrations in 3 kg of group. However, LHβ mRNA expression levels sustained increasing trends by the bodyweight. Moreover, the oocytes developed that 2 kg and 3 kg groups obtained plentiful vitellogenic oocytes while 1 kg group was still composed with greater part of pre-vitellogenic oocytes. Especially, the oocytes of 3 kg group reached over 450 μm of diameters that indicating possibility to enter the final maturations. These results suggest that the progress of pubertal development in female E. bruneus could be classify into three phases via bodyweight, including pre-puberty (1 kg), early-puberty (2 kg) and puberty (3 kg). Korean Society of Developmental Biology 2013-03 /pmc/articles/PMC4282214/ /pubmed/25949121 http://dx.doi.org/10.12717/DR.2013.17.1.055 Text en © Korean Society of Developmental Biology. All Rights Reserved http://creativecommons.org/licenses/by-nc/3.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Article
Ryu, Yong-Woon
Hur, Sang-Woo
Hur, Sung-Pyo
Lee, Chi-Hoon
Lim, Bong-Soo
Lee, Young-Don
Characterization of Pubertal Development Phases in Female Longtooth Grouper, Epinephelus bruneus via Classification of Bodyweight
title Characterization of Pubertal Development Phases in Female Longtooth Grouper, Epinephelus bruneus via Classification of Bodyweight
title_full Characterization of Pubertal Development Phases in Female Longtooth Grouper, Epinephelus bruneus via Classification of Bodyweight
title_fullStr Characterization of Pubertal Development Phases in Female Longtooth Grouper, Epinephelus bruneus via Classification of Bodyweight
title_full_unstemmed Characterization of Pubertal Development Phases in Female Longtooth Grouper, Epinephelus bruneus via Classification of Bodyweight
title_short Characterization of Pubertal Development Phases in Female Longtooth Grouper, Epinephelus bruneus via Classification of Bodyweight
title_sort characterization of pubertal development phases in female longtooth grouper, epinephelus bruneus via classification of bodyweight
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4282214/
https://www.ncbi.nlm.nih.gov/pubmed/25949121
http://dx.doi.org/10.12717/DR.2013.17.1.055
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