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Decreased Expression of Inhibitor of DNA-binding (Id) Proteins and Vascular Endothelial Growth Factor and Increased Apoptosis in Ovarian Aging

This study examined the expression of inhibitor of DNA-binding (Id) proteins and vascular endothelial growth factor (VEGF) in the ovary according to female age using a mice model as the first step in investigating the potential role of Ids and VEGF in ovarian aging. C57BL inbred female mice of three...

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Autores principales: Park, Min Jung, Park, Sea Hee, Moon, Sung Eun, Koo, Ja Seong, Moon, Hwa Sook, Joo, Bo Sun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Korean Society of Developmental Biology 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4282215/
https://www.ncbi.nlm.nih.gov/pubmed/25949117
http://dx.doi.org/10.12717/DR.2013.17.1.017
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author Park, Min Jung
Park, Sea Hee
Moon, Sung Eun
Koo, Ja Seong
Moon, Hwa Sook
Joo, Bo Sun
author_facet Park, Min Jung
Park, Sea Hee
Moon, Sung Eun
Koo, Ja Seong
Moon, Hwa Sook
Joo, Bo Sun
author_sort Park, Min Jung
collection PubMed
description This study examined the expression of inhibitor of DNA-binding (Id) proteins and vascular endothelial growth factor (VEGF) in the ovary according to female age using a mice model as the first step in investigating the potential role of Ids and VEGF in ovarian aging. C57BL inbred female mice of three age groups (6-9, 14-16, and 23-26 weeks) were injected with 5 IU pregnant mare’s serum gonadotropin (PMSG) in order to synchronize the estrus cycle. After 48 h, ovarian expression of Ids and VEGF was analyzed by reverse transcriptase-polymerase chain reaction (RT-PCR), western blot and immunohistochemistry. Ovarian apoptosis was examined by ovarian expression of Bcl-2 and Bcl-xL. Expression of Id-1 and VEGF was decreased with advancing female age, but not Id-2, Id-3, and Id-4. In particular, their expressions were significantly decreased in aged mice of 23-26 weeks compared with the young mice of 6-9 weeks (p < 0.05). In contrast, ovarian apoptosis was greatly increased in the aged mice compared to the young mice. This result suggests that Id-1 may have an implicated role in ovarian aging by associating with VEGF.
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spelling pubmed-42822152015-05-06 Decreased Expression of Inhibitor of DNA-binding (Id) Proteins and Vascular Endothelial Growth Factor and Increased Apoptosis in Ovarian Aging Park, Min Jung Park, Sea Hee Moon, Sung Eun Koo, Ja Seong Moon, Hwa Sook Joo, Bo Sun Dev Reprod Article This study examined the expression of inhibitor of DNA-binding (Id) proteins and vascular endothelial growth factor (VEGF) in the ovary according to female age using a mice model as the first step in investigating the potential role of Ids and VEGF in ovarian aging. C57BL inbred female mice of three age groups (6-9, 14-16, and 23-26 weeks) were injected with 5 IU pregnant mare’s serum gonadotropin (PMSG) in order to synchronize the estrus cycle. After 48 h, ovarian expression of Ids and VEGF was analyzed by reverse transcriptase-polymerase chain reaction (RT-PCR), western blot and immunohistochemistry. Ovarian apoptosis was examined by ovarian expression of Bcl-2 and Bcl-xL. Expression of Id-1 and VEGF was decreased with advancing female age, but not Id-2, Id-3, and Id-4. In particular, their expressions were significantly decreased in aged mice of 23-26 weeks compared with the young mice of 6-9 weeks (p < 0.05). In contrast, ovarian apoptosis was greatly increased in the aged mice compared to the young mice. This result suggests that Id-1 may have an implicated role in ovarian aging by associating with VEGF. Korean Society of Developmental Biology 2013-03 /pmc/articles/PMC4282215/ /pubmed/25949117 http://dx.doi.org/10.12717/DR.2013.17.1.017 Text en © Korean Society of Developmental Biology. All Rights Reserved http://creativecommons.org/licenses/by-nc/3.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Article
Park, Min Jung
Park, Sea Hee
Moon, Sung Eun
Koo, Ja Seong
Moon, Hwa Sook
Joo, Bo Sun
Decreased Expression of Inhibitor of DNA-binding (Id) Proteins and Vascular Endothelial Growth Factor and Increased Apoptosis in Ovarian Aging
title Decreased Expression of Inhibitor of DNA-binding (Id) Proteins and Vascular Endothelial Growth Factor and Increased Apoptosis in Ovarian Aging
title_full Decreased Expression of Inhibitor of DNA-binding (Id) Proteins and Vascular Endothelial Growth Factor and Increased Apoptosis in Ovarian Aging
title_fullStr Decreased Expression of Inhibitor of DNA-binding (Id) Proteins and Vascular Endothelial Growth Factor and Increased Apoptosis in Ovarian Aging
title_full_unstemmed Decreased Expression of Inhibitor of DNA-binding (Id) Proteins and Vascular Endothelial Growth Factor and Increased Apoptosis in Ovarian Aging
title_short Decreased Expression of Inhibitor of DNA-binding (Id) Proteins and Vascular Endothelial Growth Factor and Increased Apoptosis in Ovarian Aging
title_sort decreased expression of inhibitor of dna-binding (id) proteins and vascular endothelial growth factor and increased apoptosis in ovarian aging
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4282215/
https://www.ncbi.nlm.nih.gov/pubmed/25949117
http://dx.doi.org/10.12717/DR.2013.17.1.017
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