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Genetic Distances of Three White Clam (Meretrix lusoria) Populations Investigated by PCR Analysis

The twenty-one individuals of Meretrix lusoria were secured from Gunsan, Shinan and Yeonggwang on the coast of the Yellow Sea and the southern sea in the Korean Peninsula, respectively. Amplification of a single COI fragment (720 bp) was imagined, and no apparent size differences were observed in am...

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Autores principales: Kim, Dae-Hyun, Yoon, Jong-Man
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Korean Society of Developmental Biology 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4282255/
https://www.ncbi.nlm.nih.gov/pubmed/25949176
http://dx.doi.org/10.12717/DR.2014.18.2.089
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author Kim, Dae-Hyun
Yoon, Jong-Man
author_facet Kim, Dae-Hyun
Yoon, Jong-Man
author_sort Kim, Dae-Hyun
collection PubMed
description The twenty-one individuals of Meretrix lusoria were secured from Gunsan, Shinan and Yeonggwang on the coast of the Yellow Sea and the southern sea in the Korean Peninsula, respectively. Amplification of a single COI fragment (720 bp) was imagined, and no apparent size differences were observed in amplified fragments between Meretrix lusoria and M. petechialis individuals. The size of the DNA fragments also varied excitedly, from 200 to 1,600 bp. The oligonucleotides primer BION-08 produced the least loci (a total of 17), with an average of 2.43 in the Gunsan population, in comparison to the other primers used. Remarkably, the primer BION-13 detected 42 shared loci by the three populations, major and/or minor fragments of sizes 200 bp and 400 bp, respectively, which were identical in all samples. The dendrogram gained by the seven oligonucleotides primers highlight three genetic clusters: cluster 1 (GUNSAN 01 ~ GUNSAN 07), cluster 2 (SHINAN 08 ~ SHINAN 14) and cluster 3 (YEONGGWANG 15 ~ YEONGGWANG 21). The longest genetic distance among the twenty-one Meretrix lusoria individuals that displayed significant molecular differences was between individuals GUNSAN no. 01 and SHINAN no. 14 (genetic distance = 0.574). Comparatively, individuals of SHINAN population were fairly closely related to that of YEONGGWANG population. In this study, PCR analysis has discovered significant genetic distances between two white clam population pairs (P<0.05).
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spelling pubmed-42822552015-05-06 Genetic Distances of Three White Clam (Meretrix lusoria) Populations Investigated by PCR Analysis Kim, Dae-Hyun Yoon, Jong-Man Dev Reprod Article The twenty-one individuals of Meretrix lusoria were secured from Gunsan, Shinan and Yeonggwang on the coast of the Yellow Sea and the southern sea in the Korean Peninsula, respectively. Amplification of a single COI fragment (720 bp) was imagined, and no apparent size differences were observed in amplified fragments between Meretrix lusoria and M. petechialis individuals. The size of the DNA fragments also varied excitedly, from 200 to 1,600 bp. The oligonucleotides primer BION-08 produced the least loci (a total of 17), with an average of 2.43 in the Gunsan population, in comparison to the other primers used. Remarkably, the primer BION-13 detected 42 shared loci by the three populations, major and/or minor fragments of sizes 200 bp and 400 bp, respectively, which were identical in all samples. The dendrogram gained by the seven oligonucleotides primers highlight three genetic clusters: cluster 1 (GUNSAN 01 ~ GUNSAN 07), cluster 2 (SHINAN 08 ~ SHINAN 14) and cluster 3 (YEONGGWANG 15 ~ YEONGGWANG 21). The longest genetic distance among the twenty-one Meretrix lusoria individuals that displayed significant molecular differences was between individuals GUNSAN no. 01 and SHINAN no. 14 (genetic distance = 0.574). Comparatively, individuals of SHINAN population were fairly closely related to that of YEONGGWANG population. In this study, PCR analysis has discovered significant genetic distances between two white clam population pairs (P<0.05). Korean Society of Developmental Biology 2014-06 /pmc/articles/PMC4282255/ /pubmed/25949176 http://dx.doi.org/10.12717/DR.2014.18.2.089 Text en © Copyright A Official Journal of the Korean Society of Developmental Biology. All Rights Reserved. http://creativecommons.org/licenses/by-nc/3.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Article
Kim, Dae-Hyun
Yoon, Jong-Man
Genetic Distances of Three White Clam (Meretrix lusoria) Populations Investigated by PCR Analysis
title Genetic Distances of Three White Clam (Meretrix lusoria) Populations Investigated by PCR Analysis
title_full Genetic Distances of Three White Clam (Meretrix lusoria) Populations Investigated by PCR Analysis
title_fullStr Genetic Distances of Three White Clam (Meretrix lusoria) Populations Investigated by PCR Analysis
title_full_unstemmed Genetic Distances of Three White Clam (Meretrix lusoria) Populations Investigated by PCR Analysis
title_short Genetic Distances of Three White Clam (Meretrix lusoria) Populations Investigated by PCR Analysis
title_sort genetic distances of three white clam (meretrix lusoria) populations investigated by pcr analysis
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4282255/
https://www.ncbi.nlm.nih.gov/pubmed/25949176
http://dx.doi.org/10.12717/DR.2014.18.2.089
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