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Spino-Olivary Projections in the Rat are Natomically Separate From Postsynaptic Dorsal Column Projections

The gracile nucleus (GN) and lateral part of rostral dorsal accessory olive (rDAO) are important relays for indirect, postsynaptic dorsal column, and direct ascending pathways, respectively, that terminate as climbing fibers in the “hindlimb-receiving” parts of the C1 and C3 zones in the cerebellar...

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Autores principales: Flavell, Charlotte R, Cerminara, Nadia L, Apps, Richard, Lumb, Bridget M
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BlackWell Publishing Ltd 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4282304/
https://www.ncbi.nlm.nih.gov/pubmed/24357064
http://dx.doi.org/10.1002/cne.23527
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author Flavell, Charlotte R
Cerminara, Nadia L
Apps, Richard
Lumb, Bridget M
author_facet Flavell, Charlotte R
Cerminara, Nadia L
Apps, Richard
Lumb, Bridget M
author_sort Flavell, Charlotte R
collection PubMed
description The gracile nucleus (GN) and lateral part of rostral dorsal accessory olive (rDAO) are important relays for indirect, postsynaptic dorsal column, and direct ascending pathways, respectively, that terminate as climbing fibers in the “hindlimb-receiving” parts of the C1 and C3 zones in the cerebellar cortex. While the spinal cells of origin of that project to GN and rDAO are from largely separate territories in the spinal cord, previous studies have indicated that there could be an area of overlap between these two populations in the medial dorsal horn. Given the access of these two ascending tracts to sensory (thalamic) versus sensorimotor (precerebellar) pathways, the present study therefore addresses the important question of whether or not individual neurons have the potential to contribute axons to both ascending pathways. A double-fluorescent tracer strategy was used in rats (red Retrobeads and Fluoro-Ruby or green Retrobeads and Fluoro-Emerald) to map the spatial distribution of cells of origin of the two projections in the lumbar spinal cord. The two pathways were found to receive input from almost entirely separate territories within the lumbar cord (levels L3–L5). GN predominantly receives input from lamina IV, while rDAO receives its input from three cell populations: medial laminae V–VI, lateral lamina V, and medial laminae VII–VIII. Cells that had axons that branched to supply both GN and rDAO represented only about 1% of either single-labeled cell population. Overall, the findings therefore suggest functional independence of the two ascending pathways. J. Comp. Neurol. 522:2179–2190, 2014. © 2013 Wiley Periodicals, Inc.
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spelling pubmed-42823042015-01-15 Spino-Olivary Projections in the Rat are Natomically Separate From Postsynaptic Dorsal Column Projections Flavell, Charlotte R Cerminara, Nadia L Apps, Richard Lumb, Bridget M J Comp Neurol Research Articles The gracile nucleus (GN) and lateral part of rostral dorsal accessory olive (rDAO) are important relays for indirect, postsynaptic dorsal column, and direct ascending pathways, respectively, that terminate as climbing fibers in the “hindlimb-receiving” parts of the C1 and C3 zones in the cerebellar cortex. While the spinal cells of origin of that project to GN and rDAO are from largely separate territories in the spinal cord, previous studies have indicated that there could be an area of overlap between these two populations in the medial dorsal horn. Given the access of these two ascending tracts to sensory (thalamic) versus sensorimotor (precerebellar) pathways, the present study therefore addresses the important question of whether or not individual neurons have the potential to contribute axons to both ascending pathways. A double-fluorescent tracer strategy was used in rats (red Retrobeads and Fluoro-Ruby or green Retrobeads and Fluoro-Emerald) to map the spatial distribution of cells of origin of the two projections in the lumbar spinal cord. The two pathways were found to receive input from almost entirely separate territories within the lumbar cord (levels L3–L5). GN predominantly receives input from lamina IV, while rDAO receives its input from three cell populations: medial laminae V–VI, lateral lamina V, and medial laminae VII–VIII. Cells that had axons that branched to supply both GN and rDAO represented only about 1% of either single-labeled cell population. Overall, the findings therefore suggest functional independence of the two ascending pathways. J. Comp. Neurol. 522:2179–2190, 2014. © 2013 Wiley Periodicals, Inc. BlackWell Publishing Ltd 2013-06-15 2013-12-20 /pmc/articles/PMC4282304/ /pubmed/24357064 http://dx.doi.org/10.1002/cne.23527 Text en Copyright © 2013 Wiley Periodicals, Inc. http://creativecommons.org/licenses/by/3.0/ This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Articles
Flavell, Charlotte R
Cerminara, Nadia L
Apps, Richard
Lumb, Bridget M
Spino-Olivary Projections in the Rat are Natomically Separate From Postsynaptic Dorsal Column Projections
title Spino-Olivary Projections in the Rat are Natomically Separate From Postsynaptic Dorsal Column Projections
title_full Spino-Olivary Projections in the Rat are Natomically Separate From Postsynaptic Dorsal Column Projections
title_fullStr Spino-Olivary Projections in the Rat are Natomically Separate From Postsynaptic Dorsal Column Projections
title_full_unstemmed Spino-Olivary Projections in the Rat are Natomically Separate From Postsynaptic Dorsal Column Projections
title_short Spino-Olivary Projections in the Rat are Natomically Separate From Postsynaptic Dorsal Column Projections
title_sort spino-olivary projections in the rat are natomically separate from postsynaptic dorsal column projections
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4282304/
https://www.ncbi.nlm.nih.gov/pubmed/24357064
http://dx.doi.org/10.1002/cne.23527
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