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Enhancement of oxidative and drought tolerance in Arabidopsis by overaccumulation of antioxidant flavonoids
The notion that plants use specialized metabolism to protect against environmental stresses needs to be experimentally proven by addressing the question of whether stress tolerance by specialized metabolism is directly due to metabolites such as flavonoids. We report that flavonoids with radical sca...
Autores principales: | , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BlackWell Publishing Ltd
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4282528/ https://www.ncbi.nlm.nih.gov/pubmed/24274116 http://dx.doi.org/10.1111/tpj.12388 |
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author | Nakabayashi, Ryo Yonekura-Sakakibara, Keiko Urano, Kaoru Suzuki, Makoto Yamada, Yutaka Nishizawa, Tomoko Matsuda, Fumio Kojima, Mikiko Sakakibara, Hitoshi Shinozaki, Kazuo Michael, Anthony J Tohge, Takayuki Yamazaki, Mami Saito, Kazuki |
author_facet | Nakabayashi, Ryo Yonekura-Sakakibara, Keiko Urano, Kaoru Suzuki, Makoto Yamada, Yutaka Nishizawa, Tomoko Matsuda, Fumio Kojima, Mikiko Sakakibara, Hitoshi Shinozaki, Kazuo Michael, Anthony J Tohge, Takayuki Yamazaki, Mami Saito, Kazuki |
author_sort | Nakabayashi, Ryo |
collection | PubMed |
description | The notion that plants use specialized metabolism to protect against environmental stresses needs to be experimentally proven by addressing the question of whether stress tolerance by specialized metabolism is directly due to metabolites such as flavonoids. We report that flavonoids with radical scavenging activity mitigate against oxidative and drought stress in Arabidopsis thaliana. Metabolome and transcriptome profiling and experiments with oxidative and drought stress in wild-type, single overexpressors of MYB12/PFG1 (PRODUCTION OF FLAVONOL GLYCOSIDES1) or MYB75/PAP1 (PRODUCTION OF ANTHOCYANIN PIGMENT1), double overexpressors of MYB12 and PAP1, transparent testa4 (tt4) as a flavonoid-deficient mutant, and flavonoid-deficient MYB12 or PAP1 overexpressing lines (obtained by crossing tt4 and the individual MYB overexpressor) demonstrated that flavonoid overaccumulation was key to enhanced tolerance to such stresses. Antioxidative activity assays using 2,2-diphenyl-1-picrylhydrazyl, methyl viologen, and 3,3′-diaminobenzidine clearly showed that anthocyanin overaccumulation with strong in vitro antioxidative activity mitigated the accumulation of reactive oxygen species in vivo under oxidative and drought stress. These data confirm the usefulness of flavonoids for enhancing both biotic and abiotic stress tolerance in crops. |
format | Online Article Text |
id | pubmed-4282528 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | BlackWell Publishing Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-42825282015-01-15 Enhancement of oxidative and drought tolerance in Arabidopsis by overaccumulation of antioxidant flavonoids Nakabayashi, Ryo Yonekura-Sakakibara, Keiko Urano, Kaoru Suzuki, Makoto Yamada, Yutaka Nishizawa, Tomoko Matsuda, Fumio Kojima, Mikiko Sakakibara, Hitoshi Shinozaki, Kazuo Michael, Anthony J Tohge, Takayuki Yamazaki, Mami Saito, Kazuki Plant J Original Articles The notion that plants use specialized metabolism to protect against environmental stresses needs to be experimentally proven by addressing the question of whether stress tolerance by specialized metabolism is directly due to metabolites such as flavonoids. We report that flavonoids with radical scavenging activity mitigate against oxidative and drought stress in Arabidopsis thaliana. Metabolome and transcriptome profiling and experiments with oxidative and drought stress in wild-type, single overexpressors of MYB12/PFG1 (PRODUCTION OF FLAVONOL GLYCOSIDES1) or MYB75/PAP1 (PRODUCTION OF ANTHOCYANIN PIGMENT1), double overexpressors of MYB12 and PAP1, transparent testa4 (tt4) as a flavonoid-deficient mutant, and flavonoid-deficient MYB12 or PAP1 overexpressing lines (obtained by crossing tt4 and the individual MYB overexpressor) demonstrated that flavonoid overaccumulation was key to enhanced tolerance to such stresses. Antioxidative activity assays using 2,2-diphenyl-1-picrylhydrazyl, methyl viologen, and 3,3′-diaminobenzidine clearly showed that anthocyanin overaccumulation with strong in vitro antioxidative activity mitigated the accumulation of reactive oxygen species in vivo under oxidative and drought stress. These data confirm the usefulness of flavonoids for enhancing both biotic and abiotic stress tolerance in crops. BlackWell Publishing Ltd 2014-02 2013-12-02 /pmc/articles/PMC4282528/ /pubmed/24274116 http://dx.doi.org/10.1111/tpj.12388 Text en © 2013 The Authors. The Plant Journal © 2013 John Wiley & Sons Ltd. http://creativecommons.org/licenses/by-nc-nd/3.0/ This is an open access article under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made. |
spellingShingle | Original Articles Nakabayashi, Ryo Yonekura-Sakakibara, Keiko Urano, Kaoru Suzuki, Makoto Yamada, Yutaka Nishizawa, Tomoko Matsuda, Fumio Kojima, Mikiko Sakakibara, Hitoshi Shinozaki, Kazuo Michael, Anthony J Tohge, Takayuki Yamazaki, Mami Saito, Kazuki Enhancement of oxidative and drought tolerance in Arabidopsis by overaccumulation of antioxidant flavonoids |
title | Enhancement of oxidative and drought tolerance in Arabidopsis by overaccumulation of antioxidant flavonoids |
title_full | Enhancement of oxidative and drought tolerance in Arabidopsis by overaccumulation of antioxidant flavonoids |
title_fullStr | Enhancement of oxidative and drought tolerance in Arabidopsis by overaccumulation of antioxidant flavonoids |
title_full_unstemmed | Enhancement of oxidative and drought tolerance in Arabidopsis by overaccumulation of antioxidant flavonoids |
title_short | Enhancement of oxidative and drought tolerance in Arabidopsis by overaccumulation of antioxidant flavonoids |
title_sort | enhancement of oxidative and drought tolerance in arabidopsis by overaccumulation of antioxidant flavonoids |
topic | Original Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4282528/ https://www.ncbi.nlm.nih.gov/pubmed/24274116 http://dx.doi.org/10.1111/tpj.12388 |
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