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Arginine deprivation affects glioblastoma cell adhesion, invasiveness and actin cytoskeleton organization by impairment of β-actin arginylation
A deficit of exogenous arginine affects growth and viability of numerous cancer cells. Although arginine deprivation-based strategy is currently undergoing clinical trials, molecular mechanisms of tumor cells’ response to arginine deprivation are not yet elucidated. We have examined effects of argin...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Springer Vienna
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4282698/ https://www.ncbi.nlm.nih.gov/pubmed/25362567 http://dx.doi.org/10.1007/s00726-014-1857-1 |
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author | Pavlyk, Iuliia Rzhepetskyy, Yuriy Jagielski, Adam K. Drozak, Jakub Wasik, Anna Pereverzieva, Galyna Olchowik, Marta Kunz-Schugart, Leoni A. Stasyk, Oleh Redowicz, Maria Jolanta |
author_facet | Pavlyk, Iuliia Rzhepetskyy, Yuriy Jagielski, Adam K. Drozak, Jakub Wasik, Anna Pereverzieva, Galyna Olchowik, Marta Kunz-Schugart, Leoni A. Stasyk, Oleh Redowicz, Maria Jolanta |
author_sort | Pavlyk, Iuliia |
collection | PubMed |
description | A deficit of exogenous arginine affects growth and viability of numerous cancer cells. Although arginine deprivation-based strategy is currently undergoing clinical trials, molecular mechanisms of tumor cells’ response to arginine deprivation are not yet elucidated. We have examined effects of arginine starvation on cell motility, adhesion and invasiveness as well as on actin cytoskeleton organization of human glioblastoma cells. We observed for the first time that arginine, but not lysine, starvation affected cell morphology, significantly inhibited their motility and invasiveness, and impaired adhesion. No effects on glia cells were observed. Also, arginine deprivation in glioblastoma evoked specific changes in actin assembly, decreased β-actin filament content, and affected its N-terminal arginylation. We suggest that alterations in organization of β-actin resulted from a decrease of its arginylation could be responsible for the observed effects of arginine deprivation on cell invasiveness and migration. Our data indicate that arginine deprivation-based treatment strategies could inhibit, at least transiently, the invasion process of highly malignant brain tumors and may have a potential for combination therapy to extend overall patient survival. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1007/s00726-014-1857-1) contains supplementary material, which is available to authorized users. |
format | Online Article Text |
id | pubmed-4282698 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | Springer Vienna |
record_format | MEDLINE/PubMed |
spelling | pubmed-42826982015-01-08 Arginine deprivation affects glioblastoma cell adhesion, invasiveness and actin cytoskeleton organization by impairment of β-actin arginylation Pavlyk, Iuliia Rzhepetskyy, Yuriy Jagielski, Adam K. Drozak, Jakub Wasik, Anna Pereverzieva, Galyna Olchowik, Marta Kunz-Schugart, Leoni A. Stasyk, Oleh Redowicz, Maria Jolanta Amino Acids Original Article A deficit of exogenous arginine affects growth and viability of numerous cancer cells. Although arginine deprivation-based strategy is currently undergoing clinical trials, molecular mechanisms of tumor cells’ response to arginine deprivation are not yet elucidated. We have examined effects of arginine starvation on cell motility, adhesion and invasiveness as well as on actin cytoskeleton organization of human glioblastoma cells. We observed for the first time that arginine, but not lysine, starvation affected cell morphology, significantly inhibited their motility and invasiveness, and impaired adhesion. No effects on glia cells were observed. Also, arginine deprivation in glioblastoma evoked specific changes in actin assembly, decreased β-actin filament content, and affected its N-terminal arginylation. We suggest that alterations in organization of β-actin resulted from a decrease of its arginylation could be responsible for the observed effects of arginine deprivation on cell invasiveness and migration. Our data indicate that arginine deprivation-based treatment strategies could inhibit, at least transiently, the invasion process of highly malignant brain tumors and may have a potential for combination therapy to extend overall patient survival. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1007/s00726-014-1857-1) contains supplementary material, which is available to authorized users. Springer Vienna 2014-11-02 2015 /pmc/articles/PMC4282698/ /pubmed/25362567 http://dx.doi.org/10.1007/s00726-014-1857-1 Text en © The Author(s) 2014 https://creativecommons.org/licenses/by/4.0/ Open AccessThis article is distributed under the terms of the Creative Commons Attribution License which permits any use, distribution, and reproduction in any medium, provided the original author(s) and the source are credited. |
spellingShingle | Original Article Pavlyk, Iuliia Rzhepetskyy, Yuriy Jagielski, Adam K. Drozak, Jakub Wasik, Anna Pereverzieva, Galyna Olchowik, Marta Kunz-Schugart, Leoni A. Stasyk, Oleh Redowicz, Maria Jolanta Arginine deprivation affects glioblastoma cell adhesion, invasiveness and actin cytoskeleton organization by impairment of β-actin arginylation |
title | Arginine deprivation affects glioblastoma cell adhesion, invasiveness and actin cytoskeleton organization by impairment of β-actin arginylation |
title_full | Arginine deprivation affects glioblastoma cell adhesion, invasiveness and actin cytoskeleton organization by impairment of β-actin arginylation |
title_fullStr | Arginine deprivation affects glioblastoma cell adhesion, invasiveness and actin cytoskeleton organization by impairment of β-actin arginylation |
title_full_unstemmed | Arginine deprivation affects glioblastoma cell adhesion, invasiveness and actin cytoskeleton organization by impairment of β-actin arginylation |
title_short | Arginine deprivation affects glioblastoma cell adhesion, invasiveness and actin cytoskeleton organization by impairment of β-actin arginylation |
title_sort | arginine deprivation affects glioblastoma cell adhesion, invasiveness and actin cytoskeleton organization by impairment of β-actin arginylation |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4282698/ https://www.ncbi.nlm.nih.gov/pubmed/25362567 http://dx.doi.org/10.1007/s00726-014-1857-1 |
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