Cargando…

Arginine deprivation affects glioblastoma cell adhesion, invasiveness and actin cytoskeleton organization by impairment of β-actin arginylation

A deficit of exogenous arginine affects growth and viability of numerous cancer cells. Although arginine deprivation-based strategy is currently undergoing clinical trials, molecular mechanisms of tumor cells’ response to arginine deprivation are not yet elucidated. We have examined effects of argin...

Descripción completa

Detalles Bibliográficos
Autores principales: Pavlyk, Iuliia, Rzhepetskyy, Yuriy, Jagielski, Adam K., Drozak, Jakub, Wasik, Anna, Pereverzieva, Galyna, Olchowik, Marta, Kunz-Schugart, Leoni A., Stasyk, Oleh, Redowicz, Maria Jolanta
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Vienna 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4282698/
https://www.ncbi.nlm.nih.gov/pubmed/25362567
http://dx.doi.org/10.1007/s00726-014-1857-1
_version_ 1782351163120680960
author Pavlyk, Iuliia
Rzhepetskyy, Yuriy
Jagielski, Adam K.
Drozak, Jakub
Wasik, Anna
Pereverzieva, Galyna
Olchowik, Marta
Kunz-Schugart, Leoni A.
Stasyk, Oleh
Redowicz, Maria Jolanta
author_facet Pavlyk, Iuliia
Rzhepetskyy, Yuriy
Jagielski, Adam K.
Drozak, Jakub
Wasik, Anna
Pereverzieva, Galyna
Olchowik, Marta
Kunz-Schugart, Leoni A.
Stasyk, Oleh
Redowicz, Maria Jolanta
author_sort Pavlyk, Iuliia
collection PubMed
description A deficit of exogenous arginine affects growth and viability of numerous cancer cells. Although arginine deprivation-based strategy is currently undergoing clinical trials, molecular mechanisms of tumor cells’ response to arginine deprivation are not yet elucidated. We have examined effects of arginine starvation on cell motility, adhesion and invasiveness as well as on actin cytoskeleton organization of human glioblastoma cells. We observed for the first time that arginine, but not lysine, starvation affected cell morphology, significantly inhibited their motility and invasiveness, and impaired adhesion. No effects on glia cells were observed. Also, arginine deprivation in glioblastoma evoked specific changes in actin assembly, decreased β-actin filament content, and affected its N-terminal arginylation. We suggest that alterations in organization of β-actin resulted from a decrease of its arginylation could be responsible for the observed effects of arginine deprivation on cell invasiveness and migration. Our data indicate that arginine deprivation-based treatment strategies could inhibit, at least transiently, the invasion process of highly malignant brain tumors and may have a potential for combination therapy to extend overall patient survival. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1007/s00726-014-1857-1) contains supplementary material, which is available to authorized users.
format Online
Article
Text
id pubmed-4282698
institution National Center for Biotechnology Information
language English
publishDate 2014
publisher Springer Vienna
record_format MEDLINE/PubMed
spelling pubmed-42826982015-01-08 Arginine deprivation affects glioblastoma cell adhesion, invasiveness and actin cytoskeleton organization by impairment of β-actin arginylation Pavlyk, Iuliia Rzhepetskyy, Yuriy Jagielski, Adam K. Drozak, Jakub Wasik, Anna Pereverzieva, Galyna Olchowik, Marta Kunz-Schugart, Leoni A. Stasyk, Oleh Redowicz, Maria Jolanta Amino Acids Original Article A deficit of exogenous arginine affects growth and viability of numerous cancer cells. Although arginine deprivation-based strategy is currently undergoing clinical trials, molecular mechanisms of tumor cells’ response to arginine deprivation are not yet elucidated. We have examined effects of arginine starvation on cell motility, adhesion and invasiveness as well as on actin cytoskeleton organization of human glioblastoma cells. We observed for the first time that arginine, but not lysine, starvation affected cell morphology, significantly inhibited their motility and invasiveness, and impaired adhesion. No effects on glia cells were observed. Also, arginine deprivation in glioblastoma evoked specific changes in actin assembly, decreased β-actin filament content, and affected its N-terminal arginylation. We suggest that alterations in organization of β-actin resulted from a decrease of its arginylation could be responsible for the observed effects of arginine deprivation on cell invasiveness and migration. Our data indicate that arginine deprivation-based treatment strategies could inhibit, at least transiently, the invasion process of highly malignant brain tumors and may have a potential for combination therapy to extend overall patient survival. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1007/s00726-014-1857-1) contains supplementary material, which is available to authorized users. Springer Vienna 2014-11-02 2015 /pmc/articles/PMC4282698/ /pubmed/25362567 http://dx.doi.org/10.1007/s00726-014-1857-1 Text en © The Author(s) 2014 https://creativecommons.org/licenses/by/4.0/ Open AccessThis article is distributed under the terms of the Creative Commons Attribution License which permits any use, distribution, and reproduction in any medium, provided the original author(s) and the source are credited.
spellingShingle Original Article
Pavlyk, Iuliia
Rzhepetskyy, Yuriy
Jagielski, Adam K.
Drozak, Jakub
Wasik, Anna
Pereverzieva, Galyna
Olchowik, Marta
Kunz-Schugart, Leoni A.
Stasyk, Oleh
Redowicz, Maria Jolanta
Arginine deprivation affects glioblastoma cell adhesion, invasiveness and actin cytoskeleton organization by impairment of β-actin arginylation
title Arginine deprivation affects glioblastoma cell adhesion, invasiveness and actin cytoskeleton organization by impairment of β-actin arginylation
title_full Arginine deprivation affects glioblastoma cell adhesion, invasiveness and actin cytoskeleton organization by impairment of β-actin arginylation
title_fullStr Arginine deprivation affects glioblastoma cell adhesion, invasiveness and actin cytoskeleton organization by impairment of β-actin arginylation
title_full_unstemmed Arginine deprivation affects glioblastoma cell adhesion, invasiveness and actin cytoskeleton organization by impairment of β-actin arginylation
title_short Arginine deprivation affects glioblastoma cell adhesion, invasiveness and actin cytoskeleton organization by impairment of β-actin arginylation
title_sort arginine deprivation affects glioblastoma cell adhesion, invasiveness and actin cytoskeleton organization by impairment of β-actin arginylation
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4282698/
https://www.ncbi.nlm.nih.gov/pubmed/25362567
http://dx.doi.org/10.1007/s00726-014-1857-1
work_keys_str_mv AT pavlykiuliia argininedeprivationaffectsglioblastomacelladhesioninvasivenessandactincytoskeletonorganizationbyimpairmentofbactinarginylation
AT rzhepetskyyyuriy argininedeprivationaffectsglioblastomacelladhesioninvasivenessandactincytoskeletonorganizationbyimpairmentofbactinarginylation
AT jagielskiadamk argininedeprivationaffectsglioblastomacelladhesioninvasivenessandactincytoskeletonorganizationbyimpairmentofbactinarginylation
AT drozakjakub argininedeprivationaffectsglioblastomacelladhesioninvasivenessandactincytoskeletonorganizationbyimpairmentofbactinarginylation
AT wasikanna argininedeprivationaffectsglioblastomacelladhesioninvasivenessandactincytoskeletonorganizationbyimpairmentofbactinarginylation
AT pereverzievagalyna argininedeprivationaffectsglioblastomacelladhesioninvasivenessandactincytoskeletonorganizationbyimpairmentofbactinarginylation
AT olchowikmarta argininedeprivationaffectsglioblastomacelladhesioninvasivenessandactincytoskeletonorganizationbyimpairmentofbactinarginylation
AT kunzschugartleonia argininedeprivationaffectsglioblastomacelladhesioninvasivenessandactincytoskeletonorganizationbyimpairmentofbactinarginylation
AT stasykoleh argininedeprivationaffectsglioblastomacelladhesioninvasivenessandactincytoskeletonorganizationbyimpairmentofbactinarginylation
AT redowiczmariajolanta argininedeprivationaffectsglioblastomacelladhesioninvasivenessandactincytoskeletonorganizationbyimpairmentofbactinarginylation