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A flavonoid 3-O-glucoside:2″-O-glucosyltransferase responsible for terminal modification of pollen-specific flavonols in Arabidopsis thaliana

Flavonol 3-O-diglucosides with a 1→2 inter-glycosidic linkage are representative pollen-specific flavonols that are widely distributed in plants, but their biosynthetic genes and physiological roles are not well understood. Flavonoid analysis of four Arabidopsis floral organs (pistils, stamens, peta...

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Autores principales: Yonekura-Sakakibara, Keiko, Nakabayashi, Ryo, Sugawara, Satoko, Tohge, Takayuki, Ito, Takuya, Koyanagi, Misuzu, Kitajima, Mariko, Takayama, Hiromitsu, Saito, Kazuki
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BlackWell Publishing Ltd 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4282749/
https://www.ncbi.nlm.nih.gov/pubmed/24916675
http://dx.doi.org/10.1111/tpj.12580
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author Yonekura-Sakakibara, Keiko
Nakabayashi, Ryo
Sugawara, Satoko
Tohge, Takayuki
Ito, Takuya
Koyanagi, Misuzu
Kitajima, Mariko
Takayama, Hiromitsu
Saito, Kazuki
author_facet Yonekura-Sakakibara, Keiko
Nakabayashi, Ryo
Sugawara, Satoko
Tohge, Takayuki
Ito, Takuya
Koyanagi, Misuzu
Kitajima, Mariko
Takayama, Hiromitsu
Saito, Kazuki
author_sort Yonekura-Sakakibara, Keiko
collection PubMed
description Flavonol 3-O-diglucosides with a 1→2 inter-glycosidic linkage are representative pollen-specific flavonols that are widely distributed in plants, but their biosynthetic genes and physiological roles are not well understood. Flavonoid analysis of four Arabidopsis floral organs (pistils, stamens, petals and calyxes) and flowers of wild-type and male sterility 1 (ms1) mutants, which are defective in normal development of pollen and tapetum, showed that kaempferol/quercetin 3-O-β-d-glucopyranosyl-(1→2)-β-d-glucopyranosides accumulated in Arabidopsis pollen. Microarray data using wild-type and ms1 mutants, gene expression patterns in various organs, and phylogenetic analysis of UDP-glycosyltransferases (UGTs) suggest that UGT79B6 (At5g54010) is a key modification enzyme for determining pollen-specific flavonol structure. Kaempferol and quercetin 3-O-glucosyl-(1→2)-glucosides were absent from two independent ugt79b6 knockout mutants. Transgenic ugt79b6 mutant lines transformed with the genomic UGT79B6 gene had the same flavonoid profile as wild-type plants. Recombinant UGT79B6 protein converted kaempferol 3-O-glucoside to kaempferol 3-O-glucosyl-(1→2)-glucoside. UGT79B6 recognized 3-O-glucosylated/galactosylated anthocyanins/flavonols but not 3,5- or 3,7-diglycosylated flavonoids, and prefers UDP-glucose, indicating that UGT79B6 encodes flavonoid 3-O-glucoside:2″-O-glucosyltransferase. A UGT79B6-GUS fusion showed that UGT79B6 was localized in tapetum cells and microspores of developing anthers.
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spelling pubmed-42827492015-01-15 A flavonoid 3-O-glucoside:2″-O-glucosyltransferase responsible for terminal modification of pollen-specific flavonols in Arabidopsis thaliana Yonekura-Sakakibara, Keiko Nakabayashi, Ryo Sugawara, Satoko Tohge, Takayuki Ito, Takuya Koyanagi, Misuzu Kitajima, Mariko Takayama, Hiromitsu Saito, Kazuki Plant J Original Articles Flavonol 3-O-diglucosides with a 1→2 inter-glycosidic linkage are representative pollen-specific flavonols that are widely distributed in plants, but their biosynthetic genes and physiological roles are not well understood. Flavonoid analysis of four Arabidopsis floral organs (pistils, stamens, petals and calyxes) and flowers of wild-type and male sterility 1 (ms1) mutants, which are defective in normal development of pollen and tapetum, showed that kaempferol/quercetin 3-O-β-d-glucopyranosyl-(1→2)-β-d-glucopyranosides accumulated in Arabidopsis pollen. Microarray data using wild-type and ms1 mutants, gene expression patterns in various organs, and phylogenetic analysis of UDP-glycosyltransferases (UGTs) suggest that UGT79B6 (At5g54010) is a key modification enzyme for determining pollen-specific flavonol structure. Kaempferol and quercetin 3-O-glucosyl-(1→2)-glucosides were absent from two independent ugt79b6 knockout mutants. Transgenic ugt79b6 mutant lines transformed with the genomic UGT79B6 gene had the same flavonoid profile as wild-type plants. Recombinant UGT79B6 protein converted kaempferol 3-O-glucoside to kaempferol 3-O-glucosyl-(1→2)-glucoside. UGT79B6 recognized 3-O-glucosylated/galactosylated anthocyanins/flavonols but not 3,5- or 3,7-diglycosylated flavonoids, and prefers UDP-glucose, indicating that UGT79B6 encodes flavonoid 3-O-glucoside:2″-O-glucosyltransferase. A UGT79B6-GUS fusion showed that UGT79B6 was localized in tapetum cells and microspores of developing anthers. BlackWell Publishing Ltd 2014-09 2014-07-28 /pmc/articles/PMC4282749/ /pubmed/24916675 http://dx.doi.org/10.1111/tpj.12580 Text en © 2014 The Authors. The Plant Journal published by Society for Experimental Biology and John Wiley & Sons Ltd. http://creativecommons.org/licenses/by-nc-nd/3.0/ This is an open access article under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.
spellingShingle Original Articles
Yonekura-Sakakibara, Keiko
Nakabayashi, Ryo
Sugawara, Satoko
Tohge, Takayuki
Ito, Takuya
Koyanagi, Misuzu
Kitajima, Mariko
Takayama, Hiromitsu
Saito, Kazuki
A flavonoid 3-O-glucoside:2″-O-glucosyltransferase responsible for terminal modification of pollen-specific flavonols in Arabidopsis thaliana
title A flavonoid 3-O-glucoside:2″-O-glucosyltransferase responsible for terminal modification of pollen-specific flavonols in Arabidopsis thaliana
title_full A flavonoid 3-O-glucoside:2″-O-glucosyltransferase responsible for terminal modification of pollen-specific flavonols in Arabidopsis thaliana
title_fullStr A flavonoid 3-O-glucoside:2″-O-glucosyltransferase responsible for terminal modification of pollen-specific flavonols in Arabidopsis thaliana
title_full_unstemmed A flavonoid 3-O-glucoside:2″-O-glucosyltransferase responsible for terminal modification of pollen-specific flavonols in Arabidopsis thaliana
title_short A flavonoid 3-O-glucoside:2″-O-glucosyltransferase responsible for terminal modification of pollen-specific flavonols in Arabidopsis thaliana
title_sort flavonoid 3-o-glucoside:2″-o-glucosyltransferase responsible for terminal modification of pollen-specific flavonols in arabidopsis thaliana
topic Original Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4282749/
https://www.ncbi.nlm.nih.gov/pubmed/24916675
http://dx.doi.org/10.1111/tpj.12580
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