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Antimicrobial and Antibiofilm Activity of Chitosan on the Oral Pathogen Candida albicans

Oral candidiasis is particularly evident, not only in cancer patients receiving chemotherapy, but also in elderly people with xerostomy. In general, Candida is an opportunistic pathogen, causing infections in immunocompromised people and, in some cases, when the natural microbiota is altered. Chitos...

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Autores principales: Costa, Eduardo, Silva, Sara, Tavaria, Freni, Pintado, Manuela
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4282891/
https://www.ncbi.nlm.nih.gov/pubmed/25513734
http://dx.doi.org/10.3390/pathogens3040908
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author Costa, Eduardo
Silva, Sara
Tavaria, Freni
Pintado, Manuela
author_facet Costa, Eduardo
Silva, Sara
Tavaria, Freni
Pintado, Manuela
author_sort Costa, Eduardo
collection PubMed
description Oral candidiasis is particularly evident, not only in cancer patients receiving chemotherapy, but also in elderly people with xerostomy. In general, Candida is an opportunistic pathogen, causing infections in immunocompromised people and, in some cases, when the natural microbiota is altered. Chitosan, a natural derivative of chitin, is a polysaccharide that has been proven to possess a broad spectrum of antimicrobial activity that encompasses action against fungi, yeast and bacteria. While recent studies have revealed a significant antibiofilm activity upon several microorganisms, including C. albicans, little is known regarding the impact of chitosan upon the adhesive process or mature biofilms. With that in mind, the purpose of this work was to evaluate, in vitro, the capability of chitosan to inhibit C. albicans growth and biofilm formation. The results obtained showed that chitosan is capable of inhibiting C. albicans planktonic growth (HMW, 1 mg/mL; LMW, 3 mg/mL). Regarding biofilm growth, chitosan inhibited C. albicans adhesion (ca. 95%), biofilm formation (percentages above 90%) and reduced mature biofilms by ca. 65% and dual species biofilms (C. albicans and S. mutans) by ca. 70%. These results display the potential of this molecule to be used as an effective anti-Candida agent capable of acting upon C. albicans infections.
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spelling pubmed-42828912015-01-21 Antimicrobial and Antibiofilm Activity of Chitosan on the Oral Pathogen Candida albicans Costa, Eduardo Silva, Sara Tavaria, Freni Pintado, Manuela Pathogens Article Oral candidiasis is particularly evident, not only in cancer patients receiving chemotherapy, but also in elderly people with xerostomy. In general, Candida is an opportunistic pathogen, causing infections in immunocompromised people and, in some cases, when the natural microbiota is altered. Chitosan, a natural derivative of chitin, is a polysaccharide that has been proven to possess a broad spectrum of antimicrobial activity that encompasses action against fungi, yeast and bacteria. While recent studies have revealed a significant antibiofilm activity upon several microorganisms, including C. albicans, little is known regarding the impact of chitosan upon the adhesive process or mature biofilms. With that in mind, the purpose of this work was to evaluate, in vitro, the capability of chitosan to inhibit C. albicans growth and biofilm formation. The results obtained showed that chitosan is capable of inhibiting C. albicans planktonic growth (HMW, 1 mg/mL; LMW, 3 mg/mL). Regarding biofilm growth, chitosan inhibited C. albicans adhesion (ca. 95%), biofilm formation (percentages above 90%) and reduced mature biofilms by ca. 65% and dual species biofilms (C. albicans and S. mutans) by ca. 70%. These results display the potential of this molecule to be used as an effective anti-Candida agent capable of acting upon C. albicans infections. MDPI 2014-12-11 /pmc/articles/PMC4282891/ /pubmed/25513734 http://dx.doi.org/10.3390/pathogens3040908 Text en © 2014 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Costa, Eduardo
Silva, Sara
Tavaria, Freni
Pintado, Manuela
Antimicrobial and Antibiofilm Activity of Chitosan on the Oral Pathogen Candida albicans
title Antimicrobial and Antibiofilm Activity of Chitosan on the Oral Pathogen Candida albicans
title_full Antimicrobial and Antibiofilm Activity of Chitosan on the Oral Pathogen Candida albicans
title_fullStr Antimicrobial and Antibiofilm Activity of Chitosan on the Oral Pathogen Candida albicans
title_full_unstemmed Antimicrobial and Antibiofilm Activity of Chitosan on the Oral Pathogen Candida albicans
title_short Antimicrobial and Antibiofilm Activity of Chitosan on the Oral Pathogen Candida albicans
title_sort antimicrobial and antibiofilm activity of chitosan on the oral pathogen candida albicans
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4282891/
https://www.ncbi.nlm.nih.gov/pubmed/25513734
http://dx.doi.org/10.3390/pathogens3040908
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