Interaction between Leptospiral Lipopolysaccharide and Toll-like Receptor 2 in Pig Fibroblast Cell Line, and Inhibitory Effect of Antibody against Leptospiral Lipopolysaccharide on Interaction

Leptospiral lipopolysaccharide (L-LPS) has shown potency in activating toll-like receptor 2 (TLR2) in pig fibroblasts (PEFs_NCC1), and causes the expression of proinflammatory cytokines. However, the stimulation by L-LPS was weak eliciting the function of TLR2 sufficiently in pig innate immunity res...

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Autores principales: Guo, Yijie, Fukuda, Tomokazu, Nakamura, Shuichi, Bai, Lanlan, Xu, Jun, Kuroda, Kengo, Tomioka, Rintaro, Yoneyama, Hiroshi, Isogai, Emiko
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Asian-Australasian Association of Animal Production Societies (AAAP) and Korean Society of Animal Science and Technology (KSAST) 2015
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4283174/
https://www.ncbi.nlm.nih.gov/pubmed/25557825
http://dx.doi.org/10.5713/ajas.14.0440
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author Guo, Yijie
Fukuda, Tomokazu
Nakamura, Shuichi
Bai, Lanlan
Xu, Jun
Kuroda, Kengo
Tomioka, Rintaro
Yoneyama, Hiroshi
Isogai, Emiko
author_facet Guo, Yijie
Fukuda, Tomokazu
Nakamura, Shuichi
Bai, Lanlan
Xu, Jun
Kuroda, Kengo
Tomioka, Rintaro
Yoneyama, Hiroshi
Isogai, Emiko
author_sort Guo, Yijie
collection PubMed
description Leptospiral lipopolysaccharide (L-LPS) has shown potency in activating toll-like receptor 2 (TLR2) in pig fibroblasts (PEFs_NCC1), and causes the expression of proinflammatory cytokines. However, the stimulation by L-LPS was weak eliciting the function of TLR2 sufficiently in pig innate immunity responses during Leptospira infection. In this study, the immune response of pig embryonic fibroblast cell line (PEFs_SV40) was investigated and was found to be the high immune response, thus TLR2 is the predominate receptor of L-LPS in pig cells. Further, we found a strategy using the antibody against L-LPS, to prevent L-LPS interaction with TLR2 in pig cells which could impact on immune activation.
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spelling pubmed-42831742015-02-01 Interaction between Leptospiral Lipopolysaccharide and Toll-like Receptor 2 in Pig Fibroblast Cell Line, and Inhibitory Effect of Antibody against Leptospiral Lipopolysaccharide on Interaction Guo, Yijie Fukuda, Tomokazu Nakamura, Shuichi Bai, Lanlan Xu, Jun Kuroda, Kengo Tomioka, Rintaro Yoneyama, Hiroshi Isogai, Emiko Asian-Australas J Anim Sci Article Leptospiral lipopolysaccharide (L-LPS) has shown potency in activating toll-like receptor 2 (TLR2) in pig fibroblasts (PEFs_NCC1), and causes the expression of proinflammatory cytokines. However, the stimulation by L-LPS was weak eliciting the function of TLR2 sufficiently in pig innate immunity responses during Leptospira infection. In this study, the immune response of pig embryonic fibroblast cell line (PEFs_SV40) was investigated and was found to be the high immune response, thus TLR2 is the predominate receptor of L-LPS in pig cells. Further, we found a strategy using the antibody against L-LPS, to prevent L-LPS interaction with TLR2 in pig cells which could impact on immune activation. Asian-Australasian Association of Animal Production Societies (AAAP) and Korean Society of Animal Science and Technology (KSAST) 2015-02 /pmc/articles/PMC4283174/ /pubmed/25557825 http://dx.doi.org/10.5713/ajas.14.0440 Text en Copyright © 2015 by Asian-Australasian Journal of Animal Sciences This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License http://creativecommons.org/licenses/by-nc/3.0/ which permits unrestricted noncommercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Article
Guo, Yijie
Fukuda, Tomokazu
Nakamura, Shuichi
Bai, Lanlan
Xu, Jun
Kuroda, Kengo
Tomioka, Rintaro
Yoneyama, Hiroshi
Isogai, Emiko
Interaction between Leptospiral Lipopolysaccharide and Toll-like Receptor 2 in Pig Fibroblast Cell Line, and Inhibitory Effect of Antibody against Leptospiral Lipopolysaccharide on Interaction
title Interaction between Leptospiral Lipopolysaccharide and Toll-like Receptor 2 in Pig Fibroblast Cell Line, and Inhibitory Effect of Antibody against Leptospiral Lipopolysaccharide on Interaction
title_full Interaction between Leptospiral Lipopolysaccharide and Toll-like Receptor 2 in Pig Fibroblast Cell Line, and Inhibitory Effect of Antibody against Leptospiral Lipopolysaccharide on Interaction
title_fullStr Interaction between Leptospiral Lipopolysaccharide and Toll-like Receptor 2 in Pig Fibroblast Cell Line, and Inhibitory Effect of Antibody against Leptospiral Lipopolysaccharide on Interaction
title_full_unstemmed Interaction between Leptospiral Lipopolysaccharide and Toll-like Receptor 2 in Pig Fibroblast Cell Line, and Inhibitory Effect of Antibody against Leptospiral Lipopolysaccharide on Interaction
title_short Interaction between Leptospiral Lipopolysaccharide and Toll-like Receptor 2 in Pig Fibroblast Cell Line, and Inhibitory Effect of Antibody against Leptospiral Lipopolysaccharide on Interaction
title_sort interaction between leptospiral lipopolysaccharide and toll-like receptor 2 in pig fibroblast cell line, and inhibitory effect of antibody against leptospiral lipopolysaccharide on interaction
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4283174/
https://www.ncbi.nlm.nih.gov/pubmed/25557825
http://dx.doi.org/10.5713/ajas.14.0440
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