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Packing of large-scale chromatography columns with irregularly shaped glass based resins using a stop-flow method
Rigid chromatography resins, such as controlled pore glass based adsorbents, offer the advantage of high permeability and a linear pressure-flow relationship irrespective of column diameter which improves process time and maximizes productivity. However, the rigidity and irregularly shaped nature of...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BlackWell Publishing Ltd
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4283707/ https://www.ncbi.nlm.nih.gov/pubmed/25080096 http://dx.doi.org/10.1002/btpr.1962 |
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author | Siu, Sun Chau Chia, Celeste Mok, Yanglin Pattnaik, Priyabrata |
author_facet | Siu, Sun Chau Chia, Celeste Mok, Yanglin Pattnaik, Priyabrata |
author_sort | Siu, Sun Chau |
collection | PubMed |
description | Rigid chromatography resins, such as controlled pore glass based adsorbents, offer the advantage of high permeability and a linear pressure-flow relationship irrespective of column diameter which improves process time and maximizes productivity. However, the rigidity and irregularly shaped nature of these resins often present challenges in achieving consistent and uniform packed beds as formation of bridges between resin particles can hinder bed consolidation. The standard flow-pack method when applied to irregularly shaped particles does not yield well-consolidated packed beds, resulting in formation of a head space and increased band broadening during operation. Vibration packing methods requiring the use of pneumatically driven vibrators are recommended to achieve full packed bed consolidation but limitations in manufacturing facilities and equipment may prevent the implementation of such devices. The stop-flow packing method was developed as an improvement over the flow-pack method to overcome these limitations and to improve bed consolidation without the use of vibrating devices. Transition analysis of large-scale columns packed using the stop-flow method over multiple cycles has shown a two- to three-fold reduction of change in bed integrity values as compared to a flow-packed bed demonstrating an improvement in packed bed stability in terms of the height equivalent to a theoretical plate (HETP) and peak asymmetry (A(s)). |
format | Online Article Text |
id | pubmed-4283707 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | BlackWell Publishing Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-42837072015-01-14 Packing of large-scale chromatography columns with irregularly shaped glass based resins using a stop-flow method Siu, Sun Chau Chia, Celeste Mok, Yanglin Pattnaik, Priyabrata Biotechnol Prog Bioseparations and Downstream Processing Rigid chromatography resins, such as controlled pore glass based adsorbents, offer the advantage of high permeability and a linear pressure-flow relationship irrespective of column diameter which improves process time and maximizes productivity. However, the rigidity and irregularly shaped nature of these resins often present challenges in achieving consistent and uniform packed beds as formation of bridges between resin particles can hinder bed consolidation. The standard flow-pack method when applied to irregularly shaped particles does not yield well-consolidated packed beds, resulting in formation of a head space and increased band broadening during operation. Vibration packing methods requiring the use of pneumatically driven vibrators are recommended to achieve full packed bed consolidation but limitations in manufacturing facilities and equipment may prevent the implementation of such devices. The stop-flow packing method was developed as an improvement over the flow-pack method to overcome these limitations and to improve bed consolidation without the use of vibrating devices. Transition analysis of large-scale columns packed using the stop-flow method over multiple cycles has shown a two- to three-fold reduction of change in bed integrity values as compared to a flow-packed bed demonstrating an improvement in packed bed stability in terms of the height equivalent to a theoretical plate (HETP) and peak asymmetry (A(s)). BlackWell Publishing Ltd 2014-11 2014-08-07 /pmc/articles/PMC4283707/ /pubmed/25080096 http://dx.doi.org/10.1002/btpr.1962 Text en © 2014 American Institute of Chemical Engineers |
spellingShingle | Bioseparations and Downstream Processing Siu, Sun Chau Chia, Celeste Mok, Yanglin Pattnaik, Priyabrata Packing of large-scale chromatography columns with irregularly shaped glass based resins using a stop-flow method |
title | Packing of large-scale chromatography columns with irregularly shaped glass based resins using a stop-flow method |
title_full | Packing of large-scale chromatography columns with irregularly shaped glass based resins using a stop-flow method |
title_fullStr | Packing of large-scale chromatography columns with irregularly shaped glass based resins using a stop-flow method |
title_full_unstemmed | Packing of large-scale chromatography columns with irregularly shaped glass based resins using a stop-flow method |
title_short | Packing of large-scale chromatography columns with irregularly shaped glass based resins using a stop-flow method |
title_sort | packing of large-scale chromatography columns with irregularly shaped glass based resins using a stop-flow method |
topic | Bioseparations and Downstream Processing |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4283707/ https://www.ncbi.nlm.nih.gov/pubmed/25080096 http://dx.doi.org/10.1002/btpr.1962 |
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