Cargando…

Defense-related transcription factors WRKY70 and WRKY54 modulate osmotic stress tolerance by regulating stomatal aperture in Arabidopsis

WRKY transcription factors (TFs) have been mainly associated with plant defense, but recent studies have suggested additional roles in the regulation of other physiological processes. Here, we explored the possible contribution of two related group III WRKY TFs, WRKY70 and WRKY54, to osmotic stress...

Descripción completa

Detalles Bibliográficos
Autores principales: Li, Jing, Besseau, Sebastien, Törönen, Petri, Sipari, Nina, Kollist, Hannes, Holm, Liisa, Palva, E Tapio
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BlackWell Publishing Ltd 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4284015/
https://www.ncbi.nlm.nih.gov/pubmed/23815736
http://dx.doi.org/10.1111/nph.12378
_version_ 1782351359108972544
author Li, Jing
Besseau, Sebastien
Törönen, Petri
Sipari, Nina
Kollist, Hannes
Holm, Liisa
Palva, E Tapio
author_facet Li, Jing
Besseau, Sebastien
Törönen, Petri
Sipari, Nina
Kollist, Hannes
Holm, Liisa
Palva, E Tapio
author_sort Li, Jing
collection PubMed
description WRKY transcription factors (TFs) have been mainly associated with plant defense, but recent studies have suggested additional roles in the regulation of other physiological processes. Here, we explored the possible contribution of two related group III WRKY TFs, WRKY70 and WRKY54, to osmotic stress tolerance. These TFs are positive regulators of plant defense, and co-operate as negative regulators of salicylic acid (SA) biosynthesis and senescence. We employed single and double mutants of wrky54 and wrky70, as well as a WRKY70 overexpressor line, to explore the role of these TFs in osmotic stress (polyethylene glycol) responses. Their effect on gene expression was characterized by microarrays and verified by quantitative PCR. Stomatal phenotypes were assessed by water retention and stomatal conductance measurements. The wrky54wrky70 double mutants exhibited clearly enhanced tolerance to osmotic stress. However, gene expression analysis showed reduced induction of osmotic stress-responsive genes in addition to reduced accumulation of the osmoprotectant proline. By contrast, the enhanced tolerance was correlated with improved water retention and enhanced stomatal closure. These findings demonstrate that WRKY70 and WRKY54 co-operate as negative regulators of stomatal closure and, consequently, osmotic stress tolerance in Arabidopsis, suggesting that they have an important role, not only in plant defense, but also in abiotic stress signaling.
format Online
Article
Text
id pubmed-4284015
institution National Center for Biotechnology Information
language English
publishDate 2013
publisher BlackWell Publishing Ltd
record_format MEDLINE/PubMed
spelling pubmed-42840152015-01-14 Defense-related transcription factors WRKY70 and WRKY54 modulate osmotic stress tolerance by regulating stomatal aperture in Arabidopsis Li, Jing Besseau, Sebastien Törönen, Petri Sipari, Nina Kollist, Hannes Holm, Liisa Palva, E Tapio New Phytol Research WRKY transcription factors (TFs) have been mainly associated with plant defense, but recent studies have suggested additional roles in the regulation of other physiological processes. Here, we explored the possible contribution of two related group III WRKY TFs, WRKY70 and WRKY54, to osmotic stress tolerance. These TFs are positive regulators of plant defense, and co-operate as negative regulators of salicylic acid (SA) biosynthesis and senescence. We employed single and double mutants of wrky54 and wrky70, as well as a WRKY70 overexpressor line, to explore the role of these TFs in osmotic stress (polyethylene glycol) responses. Their effect on gene expression was characterized by microarrays and verified by quantitative PCR. Stomatal phenotypes were assessed by water retention and stomatal conductance measurements. The wrky54wrky70 double mutants exhibited clearly enhanced tolerance to osmotic stress. However, gene expression analysis showed reduced induction of osmotic stress-responsive genes in addition to reduced accumulation of the osmoprotectant proline. By contrast, the enhanced tolerance was correlated with improved water retention and enhanced stomatal closure. These findings demonstrate that WRKY70 and WRKY54 co-operate as negative regulators of stomatal closure and, consequently, osmotic stress tolerance in Arabidopsis, suggesting that they have an important role, not only in plant defense, but also in abiotic stress signaling. BlackWell Publishing Ltd 2013-10 2013-07-01 /pmc/articles/PMC4284015/ /pubmed/23815736 http://dx.doi.org/10.1111/nph.12378 Text en Copyright © 2013 New Phytologist Trust http://creativecommons.org/licenses/by/3.0/ This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research
Li, Jing
Besseau, Sebastien
Törönen, Petri
Sipari, Nina
Kollist, Hannes
Holm, Liisa
Palva, E Tapio
Defense-related transcription factors WRKY70 and WRKY54 modulate osmotic stress tolerance by regulating stomatal aperture in Arabidopsis
title Defense-related transcription factors WRKY70 and WRKY54 modulate osmotic stress tolerance by regulating stomatal aperture in Arabidopsis
title_full Defense-related transcription factors WRKY70 and WRKY54 modulate osmotic stress tolerance by regulating stomatal aperture in Arabidopsis
title_fullStr Defense-related transcription factors WRKY70 and WRKY54 modulate osmotic stress tolerance by regulating stomatal aperture in Arabidopsis
title_full_unstemmed Defense-related transcription factors WRKY70 and WRKY54 modulate osmotic stress tolerance by regulating stomatal aperture in Arabidopsis
title_short Defense-related transcription factors WRKY70 and WRKY54 modulate osmotic stress tolerance by regulating stomatal aperture in Arabidopsis
title_sort defense-related transcription factors wrky70 and wrky54 modulate osmotic stress tolerance by regulating stomatal aperture in arabidopsis
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4284015/
https://www.ncbi.nlm.nih.gov/pubmed/23815736
http://dx.doi.org/10.1111/nph.12378
work_keys_str_mv AT lijing defenserelatedtranscriptionfactorswrky70andwrky54modulateosmoticstresstolerancebyregulatingstomatalapertureinarabidopsis
AT besseausebastien defenserelatedtranscriptionfactorswrky70andwrky54modulateosmoticstresstolerancebyregulatingstomatalapertureinarabidopsis
AT toronenpetri defenserelatedtranscriptionfactorswrky70andwrky54modulateosmoticstresstolerancebyregulatingstomatalapertureinarabidopsis
AT siparinina defenserelatedtranscriptionfactorswrky70andwrky54modulateosmoticstresstolerancebyregulatingstomatalapertureinarabidopsis
AT kollisthannes defenserelatedtranscriptionfactorswrky70andwrky54modulateosmoticstresstolerancebyregulatingstomatalapertureinarabidopsis
AT holmliisa defenserelatedtranscriptionfactorswrky70andwrky54modulateosmoticstresstolerancebyregulatingstomatalapertureinarabidopsis
AT palvaetapio defenserelatedtranscriptionfactorswrky70andwrky54modulateosmoticstresstolerancebyregulatingstomatalapertureinarabidopsis