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The effect of oxygen tension on human articular chondrocyte matrix synthesis: Integration of experimental and computational approaches
Significant oxygen gradients occur within tissue engineered cartilaginous constructs. Although oxygen tension is an important limiting parameter in the development of new cartilage matrix, its precise role in matrix formation by chondrocytes remains controversial, primarily due to discrepancies in t...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BlackWell Publishing Ltd
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4284020/ https://www.ncbi.nlm.nih.gov/pubmed/24668194 http://dx.doi.org/10.1002/bit.25241 |
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author | Li, S Oreffo, ROC Sengers, BG Tare, RS |
author_facet | Li, S Oreffo, ROC Sengers, BG Tare, RS |
author_sort | Li, S |
collection | PubMed |
description | Significant oxygen gradients occur within tissue engineered cartilaginous constructs. Although oxygen tension is an important limiting parameter in the development of new cartilage matrix, its precise role in matrix formation by chondrocytes remains controversial, primarily due to discrepancies in the experimental setup applied in different studies. In this study, the specific effects of oxygen tension on the synthesis of cartilaginous matrix by human articular chondrocytes were studied using a combined experimental-computational approach in a “scaffold-free” 3D pellet culture model. Key parameters including cellular oxygen uptake rate were determined experimentally and used in conjunction with a mathematical model to estimate oxygen tension profiles in 21-day cartilaginous pellets. A threshold oxygen tension (pO(2) ≈ 8% atmospheric pressure) for human articular chondrocytes was estimated from these inferred oxygen profiles and histological analysis of pellet sections. Human articular chondrocytes that experienced oxygen tension below this threshold demonstrated enhanced proteoglycan deposition. Conversely, oxygen tension higher than the threshold favored collagen synthesis. This study has demonstrated a close relationship between oxygen tension and matrix synthesis by human articular chondrocytes in a “scaffold-free” 3D pellet culture model, providing valuable insight into the understanding and optimization of cartilage bioengineering approaches. Biotechnol. Bioeng. 2014;111: 1876–1885. |
format | Online Article Text |
id | pubmed-4284020 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | BlackWell Publishing Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-42840202015-01-14 The effect of oxygen tension on human articular chondrocyte matrix synthesis: Integration of experimental and computational approaches Li, S Oreffo, ROC Sengers, BG Tare, RS Biotechnol Bioeng Articles Significant oxygen gradients occur within tissue engineered cartilaginous constructs. Although oxygen tension is an important limiting parameter in the development of new cartilage matrix, its precise role in matrix formation by chondrocytes remains controversial, primarily due to discrepancies in the experimental setup applied in different studies. In this study, the specific effects of oxygen tension on the synthesis of cartilaginous matrix by human articular chondrocytes were studied using a combined experimental-computational approach in a “scaffold-free” 3D pellet culture model. Key parameters including cellular oxygen uptake rate were determined experimentally and used in conjunction with a mathematical model to estimate oxygen tension profiles in 21-day cartilaginous pellets. A threshold oxygen tension (pO(2) ≈ 8% atmospheric pressure) for human articular chondrocytes was estimated from these inferred oxygen profiles and histological analysis of pellet sections. Human articular chondrocytes that experienced oxygen tension below this threshold demonstrated enhanced proteoglycan deposition. Conversely, oxygen tension higher than the threshold favored collagen synthesis. This study has demonstrated a close relationship between oxygen tension and matrix synthesis by human articular chondrocytes in a “scaffold-free” 3D pellet culture model, providing valuable insight into the understanding and optimization of cartilage bioengineering approaches. Biotechnol. Bioeng. 2014;111: 1876–1885. BlackWell Publishing Ltd 2014-09 2014-05-05 /pmc/articles/PMC4284020/ /pubmed/24668194 http://dx.doi.org/10.1002/bit.25241 Text en © 2014 The Authors. Biotechnology and Bioengineering Published by Wiley Periodicals, Inc. http://creativecommons.org/licenses/by/3.0/ This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Articles Li, S Oreffo, ROC Sengers, BG Tare, RS The effect of oxygen tension on human articular chondrocyte matrix synthesis: Integration of experimental and computational approaches |
title | The effect of oxygen tension on human articular chondrocyte matrix synthesis: Integration of experimental and computational approaches |
title_full | The effect of oxygen tension on human articular chondrocyte matrix synthesis: Integration of experimental and computational approaches |
title_fullStr | The effect of oxygen tension on human articular chondrocyte matrix synthesis: Integration of experimental and computational approaches |
title_full_unstemmed | The effect of oxygen tension on human articular chondrocyte matrix synthesis: Integration of experimental and computational approaches |
title_short | The effect of oxygen tension on human articular chondrocyte matrix synthesis: Integration of experimental and computational approaches |
title_sort | effect of oxygen tension on human articular chondrocyte matrix synthesis: integration of experimental and computational approaches |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4284020/ https://www.ncbi.nlm.nih.gov/pubmed/24668194 http://dx.doi.org/10.1002/bit.25241 |
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