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Inhibitory effect of insulin-like growth factor-binding protein-7 (IGFBP7) on in vitro angiogenesis of vascular endothelial cells in the rat corpus luteum

Angiogenesis in the developing corpus luteum (CL) is a prerequisite for establishment and maintenance of an early pregnancy. To explore the physiological significance of insulin-like growth factor-binding protein-7 (IGFBP7) in the developing CL, the effects of IGFBP7 on vascular endothelial growth f...

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Autores principales: TAMURA, Kazuhiro, YOSHIE, Mikihiro, HASHIMOTO, Keisuke, TACHIKAWA, Eiichi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Society for Reproduction and Development 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4284319/
https://www.ncbi.nlm.nih.gov/pubmed/25212428
http://dx.doi.org/10.1262/jrd.2014-069
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author TAMURA, Kazuhiro
YOSHIE, Mikihiro
HASHIMOTO, Keisuke
TACHIKAWA, Eiichi
author_facet TAMURA, Kazuhiro
YOSHIE, Mikihiro
HASHIMOTO, Keisuke
TACHIKAWA, Eiichi
author_sort TAMURA, Kazuhiro
collection PubMed
description Angiogenesis in the developing corpus luteum (CL) is a prerequisite for establishment and maintenance of an early pregnancy. To explore the physiological significance of insulin-like growth factor-binding protein-7 (IGFBP7) in the developing CL, the effects of IGFBP7 on vascular endothelial growth factor (VEGFA)- and luteinizing hormone (LH)-induced in vitro tube formation were tested using isolated luteal microvascular endothelial cells (LECs). Capillary-like tube formation of LECs and their proliferation were stimulated by both VEGFA and LH. IGFBP7 treatment suppressed VEGFA- or LH-induced tube formation. The proliferation and migration of LECs, and phosphorylation of mitogen-activated protein kinase kinase and extracellular signal-regulated kinase 1/2 were inhibited by IGFBP7. Furthermore, IGFBP7 attenuated VEGFA-enhanced cyclooxygenase (COX)-2 mRNA expression and prostaglandin E(2) secretion. These findings suggest the possibility that luteal IGFBP7 secretion may suppress the stimulatory effect of VEGFA on angiogenesis in the early CL.
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spelling pubmed-42843192015-01-27 Inhibitory effect of insulin-like growth factor-binding protein-7 (IGFBP7) on in vitro angiogenesis of vascular endothelial cells in the rat corpus luteum TAMURA, Kazuhiro YOSHIE, Mikihiro HASHIMOTO, Keisuke TACHIKAWA, Eiichi J Reprod Dev Original Article Angiogenesis in the developing corpus luteum (CL) is a prerequisite for establishment and maintenance of an early pregnancy. To explore the physiological significance of insulin-like growth factor-binding protein-7 (IGFBP7) in the developing CL, the effects of IGFBP7 on vascular endothelial growth factor (VEGFA)- and luteinizing hormone (LH)-induced in vitro tube formation were tested using isolated luteal microvascular endothelial cells (LECs). Capillary-like tube formation of LECs and their proliferation were stimulated by both VEGFA and LH. IGFBP7 treatment suppressed VEGFA- or LH-induced tube formation. The proliferation and migration of LECs, and phosphorylation of mitogen-activated protein kinase kinase and extracellular signal-regulated kinase 1/2 were inhibited by IGFBP7. Furthermore, IGFBP7 attenuated VEGFA-enhanced cyclooxygenase (COX)-2 mRNA expression and prostaglandin E(2) secretion. These findings suggest the possibility that luteal IGFBP7 secretion may suppress the stimulatory effect of VEGFA on angiogenesis in the early CL. The Society for Reproduction and Development 2014-09-09 2014-12 /pmc/articles/PMC4284319/ /pubmed/25212428 http://dx.doi.org/10.1262/jrd.2014-069 Text en ©2014 Society for Reproduction and Development http://creativecommons.org/licenses/by-nc-nd/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial No Derivatives (by-nc-nd) License.
spellingShingle Original Article
TAMURA, Kazuhiro
YOSHIE, Mikihiro
HASHIMOTO, Keisuke
TACHIKAWA, Eiichi
Inhibitory effect of insulin-like growth factor-binding protein-7 (IGFBP7) on in vitro angiogenesis of vascular endothelial cells in the rat corpus luteum
title Inhibitory effect of insulin-like growth factor-binding protein-7 (IGFBP7) on in vitro angiogenesis of vascular endothelial cells in the rat corpus luteum
title_full Inhibitory effect of insulin-like growth factor-binding protein-7 (IGFBP7) on in vitro angiogenesis of vascular endothelial cells in the rat corpus luteum
title_fullStr Inhibitory effect of insulin-like growth factor-binding protein-7 (IGFBP7) on in vitro angiogenesis of vascular endothelial cells in the rat corpus luteum
title_full_unstemmed Inhibitory effect of insulin-like growth factor-binding protein-7 (IGFBP7) on in vitro angiogenesis of vascular endothelial cells in the rat corpus luteum
title_short Inhibitory effect of insulin-like growth factor-binding protein-7 (IGFBP7) on in vitro angiogenesis of vascular endothelial cells in the rat corpus luteum
title_sort inhibitory effect of insulin-like growth factor-binding protein-7 (igfbp7) on in vitro angiogenesis of vascular endothelial cells in the rat corpus luteum
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4284319/
https://www.ncbi.nlm.nih.gov/pubmed/25212428
http://dx.doi.org/10.1262/jrd.2014-069
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