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Expression and regulation of Foxa2 in the rat uterus during early pregnancy
The forkhead box a (Foxa) protein family has been found to play important roles in mammals. Recently, the expression of Foxa2 was reported in the mouse uterus, and it was reported to be involved in regulation of implantation. However, the regulation of Foxa2 expression in the uterus is still poorly...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Society for Reproduction and Development
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4284322/ https://www.ncbi.nlm.nih.gov/pubmed/25262775 http://dx.doi.org/10.1262/jrd.2014-086 |
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author | YAMAGAMI, Kazuki YAMAUCHI, Nobuhiko KUBOTA, Kaiyu NISHIMURA, Sho CHOWDHURY, Vishwajit Sur YAMANAKA, Kenichi TAKAHASHI, Masashi TABATA, Shoji HATTORI, Masa-aki |
author_facet | YAMAGAMI, Kazuki YAMAUCHI, Nobuhiko KUBOTA, Kaiyu NISHIMURA, Sho CHOWDHURY, Vishwajit Sur YAMANAKA, Kenichi TAKAHASHI, Masashi TABATA, Shoji HATTORI, Masa-aki |
author_sort | YAMAGAMI, Kazuki |
collection | PubMed |
description | The forkhead box a (Foxa) protein family has been found to play important roles in mammals. Recently, the expression of Foxa2 was reported in the mouse uterus, and it was reported to be involved in regulation of implantation. However, the regulation of Foxa2 expression in the uterus is still poorly understood. Therefore, the present study was conducted to investigate the expressional profiles of Foxa2 in the rat uterus during the estrus cycle and pregnancy. Furthermore, the effect of steroid hormones and Hedgehog protein on the expression of Foxa2 was analyzed in vivo and in vitro. In this study, the level of expression of Foxa2 was low in the rat uterus during the different stages of the estrus cycle. However, the expression increased transiently during early pregnancy at 3.5 days post coitus (dpc) and decreased at 5.5 dpc. In ovariectomized rats, P4 treatment had no effect on the expression of Foxa2 compared with the expression in control animals. Moreover, the expression of Foxa2 in cultured epithelial cells was not increased by P4 treatment in vitro. However, Foxa2 expression was significantly decreased in the rat uterus after 24 h of E2 treatment. Treatment of cells with a recombinant Hedgehog protein significantly increased the expression of Foxa2. These results suggest that the expression of Foxa2 may transiently increase just before the implantation and it may be regulated by E2 and Hedgehog protein. |
format | Online Article Text |
id | pubmed-4284322 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | The Society for Reproduction and Development |
record_format | MEDLINE/PubMed |
spelling | pubmed-42843222015-01-27 Expression and regulation of Foxa2 in the rat uterus during early pregnancy YAMAGAMI, Kazuki YAMAUCHI, Nobuhiko KUBOTA, Kaiyu NISHIMURA, Sho CHOWDHURY, Vishwajit Sur YAMANAKA, Kenichi TAKAHASHI, Masashi TABATA, Shoji HATTORI, Masa-aki J Reprod Dev Original Article The forkhead box a (Foxa) protein family has been found to play important roles in mammals. Recently, the expression of Foxa2 was reported in the mouse uterus, and it was reported to be involved in regulation of implantation. However, the regulation of Foxa2 expression in the uterus is still poorly understood. Therefore, the present study was conducted to investigate the expressional profiles of Foxa2 in the rat uterus during the estrus cycle and pregnancy. Furthermore, the effect of steroid hormones and Hedgehog protein on the expression of Foxa2 was analyzed in vivo and in vitro. In this study, the level of expression of Foxa2 was low in the rat uterus during the different stages of the estrus cycle. However, the expression increased transiently during early pregnancy at 3.5 days post coitus (dpc) and decreased at 5.5 dpc. In ovariectomized rats, P4 treatment had no effect on the expression of Foxa2 compared with the expression in control animals. Moreover, the expression of Foxa2 in cultured epithelial cells was not increased by P4 treatment in vitro. However, Foxa2 expression was significantly decreased in the rat uterus after 24 h of E2 treatment. Treatment of cells with a recombinant Hedgehog protein significantly increased the expression of Foxa2. These results suggest that the expression of Foxa2 may transiently increase just before the implantation and it may be regulated by E2 and Hedgehog protein. The Society for Reproduction and Development 2014-09-29 2014-12 /pmc/articles/PMC4284322/ /pubmed/25262775 http://dx.doi.org/10.1262/jrd.2014-086 Text en ©2014 Society for Reproduction and Development http://creativecommons.org/licenses/by-nc-nd/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial No Derivatives (by-nc-nd) License. |
spellingShingle | Original Article YAMAGAMI, Kazuki YAMAUCHI, Nobuhiko KUBOTA, Kaiyu NISHIMURA, Sho CHOWDHURY, Vishwajit Sur YAMANAKA, Kenichi TAKAHASHI, Masashi TABATA, Shoji HATTORI, Masa-aki Expression and regulation of Foxa2 in the rat uterus during early pregnancy |
title | Expression and regulation of Foxa2 in the rat uterus during early pregnancy |
title_full | Expression and regulation of Foxa2 in the rat uterus during early pregnancy |
title_fullStr | Expression and regulation of Foxa2 in the rat uterus during early pregnancy |
title_full_unstemmed | Expression and regulation of Foxa2 in the rat uterus during early pregnancy |
title_short | Expression and regulation of Foxa2 in the rat uterus during early pregnancy |
title_sort | expression and regulation of foxa2 in the rat uterus during early pregnancy |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4284322/ https://www.ncbi.nlm.nih.gov/pubmed/25262775 http://dx.doi.org/10.1262/jrd.2014-086 |
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