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A new aquareovirus causing high mortality in farmed Atlantic halibut fry in Norway

A new aquareovirus was isolated from cultured Atlantic halibut (Hippoglossus hippoglossus) fry at a facility where massive mortalities had occurred during the start-feeding phase. The same virus was also detected in juveniles (about 10 grams) of the 2013 generation at two other production sites, but...

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Autores principales: Blindheim, Steffen, Nylund, Are, Watanabe, Kuninori, Plarre, Heidrun, Erstad, Børre, Nylund, Stian
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Vienna 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4284399/
https://www.ncbi.nlm.nih.gov/pubmed/25348270
http://dx.doi.org/10.1007/s00705-014-2235-8
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author Blindheim, Steffen
Nylund, Are
Watanabe, Kuninori
Plarre, Heidrun
Erstad, Børre
Nylund, Stian
author_facet Blindheim, Steffen
Nylund, Are
Watanabe, Kuninori
Plarre, Heidrun
Erstad, Børre
Nylund, Stian
author_sort Blindheim, Steffen
collection PubMed
description A new aquareovirus was isolated from cultured Atlantic halibut (Hippoglossus hippoglossus) fry at a facility where massive mortalities had occurred during the start-feeding phase. The same virus was also detected in juveniles (about 10 grams) of the 2013 generation at two other production sites, but not in larger fish from generations 2007–2012. The virus replicated in BF-2 and CHSE-214 cell cultures and produced syncytia and plaque-like cytopathic effects. This Atlantic halibut reovirus (AHRV) was associated with necrosis of the liver and pancreas, syncytium formation in these tissues, and distinct viroplasm areas within the syncytium in halibut fry. Transmission electron microscopy revealed that the viroplasm contained virions, non-enveloped, icosahedral particles approximately 70 nm in diameter with a double capsid layer, amorphous material, and tubular structures. The RNA-dependent RNA polymerase (RdRp) gene from the AHRV isolates showed the highest amino acid sequence identity (80 %) to an isolate belonging to the species Aquareovirus A, Atlantic salmon reovirus TS (ASRV-TS). A partial sequence from the putative fusion-associated small transmembrane (FAST) protein of AHRV was obtained, and this sequence showed the highest amino acid sequence identity (46.8 %) to Green River Chinook virus which is an unassigned member of the genus Aquareovirus, while a comparison with isolates belonging to the species Aquareovirus A showed <33 % identity. A proper assessment of the relationship of AHRV to all members of the genus Aquareovirus, however, is hampered by the absence of genetic data from members of several Aquareovirus species. AHRV is the first aquareovirus isolated from a marine coldwater fish species and the second reovirus detected in farmed fish in Norway. A similar disease of halibut fry, as described in this paper, has also been described in halibut production facilities in Canada and Scotland.
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spelling pubmed-42843992015-01-12 A new aquareovirus causing high mortality in farmed Atlantic halibut fry in Norway Blindheim, Steffen Nylund, Are Watanabe, Kuninori Plarre, Heidrun Erstad, Børre Nylund, Stian Arch Virol Original Article A new aquareovirus was isolated from cultured Atlantic halibut (Hippoglossus hippoglossus) fry at a facility where massive mortalities had occurred during the start-feeding phase. The same virus was also detected in juveniles (about 10 grams) of the 2013 generation at two other production sites, but not in larger fish from generations 2007–2012. The virus replicated in BF-2 and CHSE-214 cell cultures and produced syncytia and plaque-like cytopathic effects. This Atlantic halibut reovirus (AHRV) was associated with necrosis of the liver and pancreas, syncytium formation in these tissues, and distinct viroplasm areas within the syncytium in halibut fry. Transmission electron microscopy revealed that the viroplasm contained virions, non-enveloped, icosahedral particles approximately 70 nm in diameter with a double capsid layer, amorphous material, and tubular structures. The RNA-dependent RNA polymerase (RdRp) gene from the AHRV isolates showed the highest amino acid sequence identity (80 %) to an isolate belonging to the species Aquareovirus A, Atlantic salmon reovirus TS (ASRV-TS). A partial sequence from the putative fusion-associated small transmembrane (FAST) protein of AHRV was obtained, and this sequence showed the highest amino acid sequence identity (46.8 %) to Green River Chinook virus which is an unassigned member of the genus Aquareovirus, while a comparison with isolates belonging to the species Aquareovirus A showed <33 % identity. A proper assessment of the relationship of AHRV to all members of the genus Aquareovirus, however, is hampered by the absence of genetic data from members of several Aquareovirus species. AHRV is the first aquareovirus isolated from a marine coldwater fish species and the second reovirus detected in farmed fish in Norway. A similar disease of halibut fry, as described in this paper, has also been described in halibut production facilities in Canada and Scotland. Springer Vienna 2014-10-28 2015 /pmc/articles/PMC4284399/ /pubmed/25348270 http://dx.doi.org/10.1007/s00705-014-2235-8 Text en © The Author(s) 2014 https://creativecommons.org/licenses/by/4.0/ Open AccessThis article is distributed under the terms of the Creative Commons Attribution License which permits any use, distribution, and reproduction in any medium, provided the original author(s) and the source are credited.
spellingShingle Original Article
Blindheim, Steffen
Nylund, Are
Watanabe, Kuninori
Plarre, Heidrun
Erstad, Børre
Nylund, Stian
A new aquareovirus causing high mortality in farmed Atlantic halibut fry in Norway
title A new aquareovirus causing high mortality in farmed Atlantic halibut fry in Norway
title_full A new aquareovirus causing high mortality in farmed Atlantic halibut fry in Norway
title_fullStr A new aquareovirus causing high mortality in farmed Atlantic halibut fry in Norway
title_full_unstemmed A new aquareovirus causing high mortality in farmed Atlantic halibut fry in Norway
title_short A new aquareovirus causing high mortality in farmed Atlantic halibut fry in Norway
title_sort new aquareovirus causing high mortality in farmed atlantic halibut fry in norway
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4284399/
https://www.ncbi.nlm.nih.gov/pubmed/25348270
http://dx.doi.org/10.1007/s00705-014-2235-8
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