Cargando…

Paternal heterochromatin formation in human embryos is H3K9/HP1 directed and primed by sperm-derived histone modifications

The different configurations of maternal and paternal chromatin, acquired during oogenesis and spermatogenesis, have to be rearranged after fertilization to form a functional embryonic genome. In the paternal genome, nucleosomal chromatin domains are re-established after the protamine-to-histone exc...

Descripción completa

Detalles Bibliográficos
Autores principales: van de Werken, Christine, van der Heijden, Godfried W., Eleveld, Cindy, Teeuwssen, Miriam, Albert, Mareike, Baarends, Willy M., Laven, Joop S. E., Peters, Antoine H. F. M., Baart, Esther B.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Pub. Group 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4284653/
https://www.ncbi.nlm.nih.gov/pubmed/25519718
http://dx.doi.org/10.1038/ncomms6868
_version_ 1782351419613904896
author van de Werken, Christine
van der Heijden, Godfried W.
Eleveld, Cindy
Teeuwssen, Miriam
Albert, Mareike
Baarends, Willy M.
Laven, Joop S. E.
Peters, Antoine H. F. M.
Baart, Esther B.
author_facet van de Werken, Christine
van der Heijden, Godfried W.
Eleveld, Cindy
Teeuwssen, Miriam
Albert, Mareike
Baarends, Willy M.
Laven, Joop S. E.
Peters, Antoine H. F. M.
Baart, Esther B.
author_sort van de Werken, Christine
collection PubMed
description The different configurations of maternal and paternal chromatin, acquired during oogenesis and spermatogenesis, have to be rearranged after fertilization to form a functional embryonic genome. In the paternal genome, nucleosomal chromatin domains are re-established after the protamine-to-histone exchange. We investigated the formation of constitutive heterochromatin (cHC) in human preimplantation embryos. Our results show that histones carrying canonical cHC modifications are retained in cHC regions of sperm chromatin. These modified histones are transmitted to the oocyte and contribute to the formation of paternal embryonic cHC. Subsequently, the modifications are recognized by the H3K9/HP1 pathway maternal chromatin modifiers and propagated over the embryonic cleavage divisions. These results are in contrast to what has been described for mouse embryos, in which paternal cHC lacks canonical modifications and is initially established by Polycomb group proteins. Our results show intergenerational epigenetic inheritance of the cHC structure in human embryos.
format Online
Article
Text
id pubmed-4284653
institution National Center for Biotechnology Information
language English
publishDate 2014
publisher Nature Pub. Group
record_format MEDLINE/PubMed
spelling pubmed-42846532015-01-13 Paternal heterochromatin formation in human embryos is H3K9/HP1 directed and primed by sperm-derived histone modifications van de Werken, Christine van der Heijden, Godfried W. Eleveld, Cindy Teeuwssen, Miriam Albert, Mareike Baarends, Willy M. Laven, Joop S. E. Peters, Antoine H. F. M. Baart, Esther B. Nat Commun Article The different configurations of maternal and paternal chromatin, acquired during oogenesis and spermatogenesis, have to be rearranged after fertilization to form a functional embryonic genome. In the paternal genome, nucleosomal chromatin domains are re-established after the protamine-to-histone exchange. We investigated the formation of constitutive heterochromatin (cHC) in human preimplantation embryos. Our results show that histones carrying canonical cHC modifications are retained in cHC regions of sperm chromatin. These modified histones are transmitted to the oocyte and contribute to the formation of paternal embryonic cHC. Subsequently, the modifications are recognized by the H3K9/HP1 pathway maternal chromatin modifiers and propagated over the embryonic cleavage divisions. These results are in contrast to what has been described for mouse embryos, in which paternal cHC lacks canonical modifications and is initially established by Polycomb group proteins. Our results show intergenerational epigenetic inheritance of the cHC structure in human embryos. Nature Pub. Group 2014-12-18 /pmc/articles/PMC4284653/ /pubmed/25519718 http://dx.doi.org/10.1038/ncomms6868 Text en Copyright © 2014, Nature Publishing Group, a division of Macmillan Publishers Limited. All Rights Reserved. http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/>
spellingShingle Article
van de Werken, Christine
van der Heijden, Godfried W.
Eleveld, Cindy
Teeuwssen, Miriam
Albert, Mareike
Baarends, Willy M.
Laven, Joop S. E.
Peters, Antoine H. F. M.
Baart, Esther B.
Paternal heterochromatin formation in human embryos is H3K9/HP1 directed and primed by sperm-derived histone modifications
title Paternal heterochromatin formation in human embryos is H3K9/HP1 directed and primed by sperm-derived histone modifications
title_full Paternal heterochromatin formation in human embryos is H3K9/HP1 directed and primed by sperm-derived histone modifications
title_fullStr Paternal heterochromatin formation in human embryos is H3K9/HP1 directed and primed by sperm-derived histone modifications
title_full_unstemmed Paternal heterochromatin formation in human embryos is H3K9/HP1 directed and primed by sperm-derived histone modifications
title_short Paternal heterochromatin formation in human embryos is H3K9/HP1 directed and primed by sperm-derived histone modifications
title_sort paternal heterochromatin formation in human embryos is h3k9/hp1 directed and primed by sperm-derived histone modifications
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4284653/
https://www.ncbi.nlm.nih.gov/pubmed/25519718
http://dx.doi.org/10.1038/ncomms6868
work_keys_str_mv AT vandewerkenchristine paternalheterochromatinformationinhumanembryosish3k9hp1directedandprimedbyspermderivedhistonemodifications
AT vanderheijdengodfriedw paternalheterochromatinformationinhumanembryosish3k9hp1directedandprimedbyspermderivedhistonemodifications
AT eleveldcindy paternalheterochromatinformationinhumanembryosish3k9hp1directedandprimedbyspermderivedhistonemodifications
AT teeuwssenmiriam paternalheterochromatinformationinhumanembryosish3k9hp1directedandprimedbyspermderivedhistonemodifications
AT albertmareike paternalheterochromatinformationinhumanembryosish3k9hp1directedandprimedbyspermderivedhistonemodifications
AT baarendswillym paternalheterochromatinformationinhumanembryosish3k9hp1directedandprimedbyspermderivedhistonemodifications
AT lavenjoopse paternalheterochromatinformationinhumanembryosish3k9hp1directedandprimedbyspermderivedhistonemodifications
AT petersantoinehfm paternalheterochromatinformationinhumanembryosish3k9hp1directedandprimedbyspermderivedhistonemodifications
AT baartestherb paternalheterochromatinformationinhumanembryosish3k9hp1directedandprimedbyspermderivedhistonemodifications