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Paternal heterochromatin formation in human embryos is H3K9/HP1 directed and primed by sperm-derived histone modifications
The different configurations of maternal and paternal chromatin, acquired during oogenesis and spermatogenesis, have to be rearranged after fertilization to form a functional embryonic genome. In the paternal genome, nucleosomal chromatin domains are re-established after the protamine-to-histone exc...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Pub. Group
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4284653/ https://www.ncbi.nlm.nih.gov/pubmed/25519718 http://dx.doi.org/10.1038/ncomms6868 |
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author | van de Werken, Christine van der Heijden, Godfried W. Eleveld, Cindy Teeuwssen, Miriam Albert, Mareike Baarends, Willy M. Laven, Joop S. E. Peters, Antoine H. F. M. Baart, Esther B. |
author_facet | van de Werken, Christine van der Heijden, Godfried W. Eleveld, Cindy Teeuwssen, Miriam Albert, Mareike Baarends, Willy M. Laven, Joop S. E. Peters, Antoine H. F. M. Baart, Esther B. |
author_sort | van de Werken, Christine |
collection | PubMed |
description | The different configurations of maternal and paternal chromatin, acquired during oogenesis and spermatogenesis, have to be rearranged after fertilization to form a functional embryonic genome. In the paternal genome, nucleosomal chromatin domains are re-established after the protamine-to-histone exchange. We investigated the formation of constitutive heterochromatin (cHC) in human preimplantation embryos. Our results show that histones carrying canonical cHC modifications are retained in cHC regions of sperm chromatin. These modified histones are transmitted to the oocyte and contribute to the formation of paternal embryonic cHC. Subsequently, the modifications are recognized by the H3K9/HP1 pathway maternal chromatin modifiers and propagated over the embryonic cleavage divisions. These results are in contrast to what has been described for mouse embryos, in which paternal cHC lacks canonical modifications and is initially established by Polycomb group proteins. Our results show intergenerational epigenetic inheritance of the cHC structure in human embryos. |
format | Online Article Text |
id | pubmed-4284653 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | Nature Pub. Group |
record_format | MEDLINE/PubMed |
spelling | pubmed-42846532015-01-13 Paternal heterochromatin formation in human embryos is H3K9/HP1 directed and primed by sperm-derived histone modifications van de Werken, Christine van der Heijden, Godfried W. Eleveld, Cindy Teeuwssen, Miriam Albert, Mareike Baarends, Willy M. Laven, Joop S. E. Peters, Antoine H. F. M. Baart, Esther B. Nat Commun Article The different configurations of maternal and paternal chromatin, acquired during oogenesis and spermatogenesis, have to be rearranged after fertilization to form a functional embryonic genome. In the paternal genome, nucleosomal chromatin domains are re-established after the protamine-to-histone exchange. We investigated the formation of constitutive heterochromatin (cHC) in human preimplantation embryos. Our results show that histones carrying canonical cHC modifications are retained in cHC regions of sperm chromatin. These modified histones are transmitted to the oocyte and contribute to the formation of paternal embryonic cHC. Subsequently, the modifications are recognized by the H3K9/HP1 pathway maternal chromatin modifiers and propagated over the embryonic cleavage divisions. These results are in contrast to what has been described for mouse embryos, in which paternal cHC lacks canonical modifications and is initially established by Polycomb group proteins. Our results show intergenerational epigenetic inheritance of the cHC structure in human embryos. Nature Pub. Group 2014-12-18 /pmc/articles/PMC4284653/ /pubmed/25519718 http://dx.doi.org/10.1038/ncomms6868 Text en Copyright © 2014, Nature Publishing Group, a division of Macmillan Publishers Limited. All Rights Reserved. http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/> |
spellingShingle | Article van de Werken, Christine van der Heijden, Godfried W. Eleveld, Cindy Teeuwssen, Miriam Albert, Mareike Baarends, Willy M. Laven, Joop S. E. Peters, Antoine H. F. M. Baart, Esther B. Paternal heterochromatin formation in human embryos is H3K9/HP1 directed and primed by sperm-derived histone modifications |
title | Paternal heterochromatin formation in human embryos is H3K9/HP1 directed and primed by sperm-derived histone modifications |
title_full | Paternal heterochromatin formation in human embryos is H3K9/HP1 directed and primed by sperm-derived histone modifications |
title_fullStr | Paternal heterochromatin formation in human embryos is H3K9/HP1 directed and primed by sperm-derived histone modifications |
title_full_unstemmed | Paternal heterochromatin formation in human embryos is H3K9/HP1 directed and primed by sperm-derived histone modifications |
title_short | Paternal heterochromatin formation in human embryos is H3K9/HP1 directed and primed by sperm-derived histone modifications |
title_sort | paternal heterochromatin formation in human embryos is h3k9/hp1 directed and primed by sperm-derived histone modifications |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4284653/ https://www.ncbi.nlm.nih.gov/pubmed/25519718 http://dx.doi.org/10.1038/ncomms6868 |
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