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Transformation of intestinal stem cells into gastric stem cells on loss of transcription factor Cdx2

The endodermal lining of the adult gastro-intestinal tract harbours stem cells that are responsible for the day-to-day regeneration of the epithelium. Stem cells residing in the pyloric glands of the stomach and in the small intestinal crypts differ in their differentiation programme and in the gene...

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Autores principales: Simmini, Salvatore, Bialecka, Monika, Huch, Meritxell, Kester, Lennart, van de Wetering, Marc, Sato, Toshiro, Beck, Felix, van Oudenaarden, Alexander, Clevers, Hans, Deschamps, Jacqueline
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Pub. Group 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4284662/
https://www.ncbi.nlm.nih.gov/pubmed/25500896
http://dx.doi.org/10.1038/ncomms6728
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author Simmini, Salvatore
Bialecka, Monika
Huch, Meritxell
Kester, Lennart
van de Wetering, Marc
Sato, Toshiro
Beck, Felix
van Oudenaarden, Alexander
Clevers, Hans
Deschamps, Jacqueline
author_facet Simmini, Salvatore
Bialecka, Monika
Huch, Meritxell
Kester, Lennart
van de Wetering, Marc
Sato, Toshiro
Beck, Felix
van Oudenaarden, Alexander
Clevers, Hans
Deschamps, Jacqueline
author_sort Simmini, Salvatore
collection PubMed
description The endodermal lining of the adult gastro-intestinal tract harbours stem cells that are responsible for the day-to-day regeneration of the epithelium. Stem cells residing in the pyloric glands of the stomach and in the small intestinal crypts differ in their differentiation programme and in the gene repertoire that they express. Both types of stem cells have been shown to grow from single cells into 3D structures (organoids) in vitro. We show that single adult Lgr5-positive stem cells, isolated from small intestinal organoids, require Cdx2 to maintain their intestinal identity and are converted cell-autonomously into pyloric stem cells in the absence of this transcription factor. Clonal descendants of Cdx2(null) small intestinal stem cells enter the gastric differentiation program instead of producing intestinal derivatives. We show that the intestinal genetic programme is critically dependent on the single transcription factor encoding gene Cdx2.
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spelling pubmed-42846622015-01-13 Transformation of intestinal stem cells into gastric stem cells on loss of transcription factor Cdx2 Simmini, Salvatore Bialecka, Monika Huch, Meritxell Kester, Lennart van de Wetering, Marc Sato, Toshiro Beck, Felix van Oudenaarden, Alexander Clevers, Hans Deschamps, Jacqueline Nat Commun Article The endodermal lining of the adult gastro-intestinal tract harbours stem cells that are responsible for the day-to-day regeneration of the epithelium. Stem cells residing in the pyloric glands of the stomach and in the small intestinal crypts differ in their differentiation programme and in the gene repertoire that they express. Both types of stem cells have been shown to grow from single cells into 3D structures (organoids) in vitro. We show that single adult Lgr5-positive stem cells, isolated from small intestinal organoids, require Cdx2 to maintain their intestinal identity and are converted cell-autonomously into pyloric stem cells in the absence of this transcription factor. Clonal descendants of Cdx2(null) small intestinal stem cells enter the gastric differentiation program instead of producing intestinal derivatives. We show that the intestinal genetic programme is critically dependent on the single transcription factor encoding gene Cdx2. Nature Pub. Group 2014-12-11 /pmc/articles/PMC4284662/ /pubmed/25500896 http://dx.doi.org/10.1038/ncomms6728 Text en Copyright © 2014, Nature Publishing Group, a division of Macmillan Publishers Limited. All Rights Reserved. http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Simmini, Salvatore
Bialecka, Monika
Huch, Meritxell
Kester, Lennart
van de Wetering, Marc
Sato, Toshiro
Beck, Felix
van Oudenaarden, Alexander
Clevers, Hans
Deschamps, Jacqueline
Transformation of intestinal stem cells into gastric stem cells on loss of transcription factor Cdx2
title Transformation of intestinal stem cells into gastric stem cells on loss of transcription factor Cdx2
title_full Transformation of intestinal stem cells into gastric stem cells on loss of transcription factor Cdx2
title_fullStr Transformation of intestinal stem cells into gastric stem cells on loss of transcription factor Cdx2
title_full_unstemmed Transformation of intestinal stem cells into gastric stem cells on loss of transcription factor Cdx2
title_short Transformation of intestinal stem cells into gastric stem cells on loss of transcription factor Cdx2
title_sort transformation of intestinal stem cells into gastric stem cells on loss of transcription factor cdx2
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4284662/
https://www.ncbi.nlm.nih.gov/pubmed/25500896
http://dx.doi.org/10.1038/ncomms6728
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