Cargando…

Sterols from Mytilidae Show Anti-Aging and Neuroprotective Effects via Anti-Oxidative Activity

For screening anti-aging samples from marine natural products, K6001 yeast strain was employed as a bioassay system. The active mussel extract was separated to give an active sterol fraction (SF). SF was further purified, and four sterol compounds were obtained. Their structures were determined to b...

Descripción completa

Detalles Bibliográficos
Autores principales: Sun, Yujuan, Lin, Yanfei, Cao, Xueli, Xiang, Lan, Qi, Jianhua
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4284670/
https://www.ncbi.nlm.nih.gov/pubmed/25429428
http://dx.doi.org/10.3390/ijms151221660
_version_ 1782351425538359296
author Sun, Yujuan
Lin, Yanfei
Cao, Xueli
Xiang, Lan
Qi, Jianhua
author_facet Sun, Yujuan
Lin, Yanfei
Cao, Xueli
Xiang, Lan
Qi, Jianhua
author_sort Sun, Yujuan
collection PubMed
description For screening anti-aging samples from marine natural products, K6001 yeast strain was employed as a bioassay system. The active mussel extract was separated to give an active sterol fraction (SF). SF was further purified, and four sterol compounds were obtained. Their structures were determined to be cholesterol (CHOL), brassicasterol, crinosterol, and 24-methylenecholesterol. All compounds showed similar anti-aging activity. To understand the action mechanism involved, anti-oxidative experiments, reactive oxygen species (ROS) assays, and malondialdehyde (MDA) tests were performed on the most abundant compound, CHOL. Results indicated that treatment with CHOL increases the survival rate of yeast under oxidative stress and decreases ROS and MDA levels. In addition, mutations of uth1, skn7, sod1, and sod2, which feature a K6001 background, were employed and the lifespans of the mutations were not affected by CHOL. These results demonstrate that CHOL exerts anti-aging effects via anti-oxidative stress. Based on the connection between neuroprotection and anti-aging, neuroprotective experiments were performed in PC12 cells. Paraquat was used to induce oxidative stress and the results showed that the CHOL and SF protect the PC12 cells from the injury induced by paraquat. In addition, these substance exhibited nerve growth factor (NGF) mimic activities again confirmed their neuroprotective function.
format Online
Article
Text
id pubmed-4284670
institution National Center for Biotechnology Information
language English
publishDate 2014
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-42846702015-01-21 Sterols from Mytilidae Show Anti-Aging and Neuroprotective Effects via Anti-Oxidative Activity Sun, Yujuan Lin, Yanfei Cao, Xueli Xiang, Lan Qi, Jianhua Int J Mol Sci Article For screening anti-aging samples from marine natural products, K6001 yeast strain was employed as a bioassay system. The active mussel extract was separated to give an active sterol fraction (SF). SF was further purified, and four sterol compounds were obtained. Their structures were determined to be cholesterol (CHOL), brassicasterol, crinosterol, and 24-methylenecholesterol. All compounds showed similar anti-aging activity. To understand the action mechanism involved, anti-oxidative experiments, reactive oxygen species (ROS) assays, and malondialdehyde (MDA) tests were performed on the most abundant compound, CHOL. Results indicated that treatment with CHOL increases the survival rate of yeast under oxidative stress and decreases ROS and MDA levels. In addition, mutations of uth1, skn7, sod1, and sod2, which feature a K6001 background, were employed and the lifespans of the mutations were not affected by CHOL. These results demonstrate that CHOL exerts anti-aging effects via anti-oxidative stress. Based on the connection between neuroprotection and anti-aging, neuroprotective experiments were performed in PC12 cells. Paraquat was used to induce oxidative stress and the results showed that the CHOL and SF protect the PC12 cells from the injury induced by paraquat. In addition, these substance exhibited nerve growth factor (NGF) mimic activities again confirmed their neuroprotective function. MDPI 2014-11-25 /pmc/articles/PMC4284670/ /pubmed/25429428 http://dx.doi.org/10.3390/ijms151221660 Text en © 2014 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Sun, Yujuan
Lin, Yanfei
Cao, Xueli
Xiang, Lan
Qi, Jianhua
Sterols from Mytilidae Show Anti-Aging and Neuroprotective Effects via Anti-Oxidative Activity
title Sterols from Mytilidae Show Anti-Aging and Neuroprotective Effects via Anti-Oxidative Activity
title_full Sterols from Mytilidae Show Anti-Aging and Neuroprotective Effects via Anti-Oxidative Activity
title_fullStr Sterols from Mytilidae Show Anti-Aging and Neuroprotective Effects via Anti-Oxidative Activity
title_full_unstemmed Sterols from Mytilidae Show Anti-Aging and Neuroprotective Effects via Anti-Oxidative Activity
title_short Sterols from Mytilidae Show Anti-Aging and Neuroprotective Effects via Anti-Oxidative Activity
title_sort sterols from mytilidae show anti-aging and neuroprotective effects via anti-oxidative activity
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4284670/
https://www.ncbi.nlm.nih.gov/pubmed/25429428
http://dx.doi.org/10.3390/ijms151221660
work_keys_str_mv AT sunyujuan sterolsfrommytilidaeshowantiagingandneuroprotectiveeffectsviaantioxidativeactivity
AT linyanfei sterolsfrommytilidaeshowantiagingandneuroprotectiveeffectsviaantioxidativeactivity
AT caoxueli sterolsfrommytilidaeshowantiagingandneuroprotectiveeffectsviaantioxidativeactivity
AT xianglan sterolsfrommytilidaeshowantiagingandneuroprotectiveeffectsviaantioxidativeactivity
AT qijianhua sterolsfrommytilidaeshowantiagingandneuroprotectiveeffectsviaantioxidativeactivity