Cargando…
Sterols from Mytilidae Show Anti-Aging and Neuroprotective Effects via Anti-Oxidative Activity
For screening anti-aging samples from marine natural products, K6001 yeast strain was employed as a bioassay system. The active mussel extract was separated to give an active sterol fraction (SF). SF was further purified, and four sterol compounds were obtained. Their structures were determined to b...
Autores principales: | , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2014
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4284670/ https://www.ncbi.nlm.nih.gov/pubmed/25429428 http://dx.doi.org/10.3390/ijms151221660 |
_version_ | 1782351425538359296 |
---|---|
author | Sun, Yujuan Lin, Yanfei Cao, Xueli Xiang, Lan Qi, Jianhua |
author_facet | Sun, Yujuan Lin, Yanfei Cao, Xueli Xiang, Lan Qi, Jianhua |
author_sort | Sun, Yujuan |
collection | PubMed |
description | For screening anti-aging samples from marine natural products, K6001 yeast strain was employed as a bioassay system. The active mussel extract was separated to give an active sterol fraction (SF). SF was further purified, and four sterol compounds were obtained. Their structures were determined to be cholesterol (CHOL), brassicasterol, crinosterol, and 24-methylenecholesterol. All compounds showed similar anti-aging activity. To understand the action mechanism involved, anti-oxidative experiments, reactive oxygen species (ROS) assays, and malondialdehyde (MDA) tests were performed on the most abundant compound, CHOL. Results indicated that treatment with CHOL increases the survival rate of yeast under oxidative stress and decreases ROS and MDA levels. In addition, mutations of uth1, skn7, sod1, and sod2, which feature a K6001 background, were employed and the lifespans of the mutations were not affected by CHOL. These results demonstrate that CHOL exerts anti-aging effects via anti-oxidative stress. Based on the connection between neuroprotection and anti-aging, neuroprotective experiments were performed in PC12 cells. Paraquat was used to induce oxidative stress and the results showed that the CHOL and SF protect the PC12 cells from the injury induced by paraquat. In addition, these substance exhibited nerve growth factor (NGF) mimic activities again confirmed their neuroprotective function. |
format | Online Article Text |
id | pubmed-4284670 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-42846702015-01-21 Sterols from Mytilidae Show Anti-Aging and Neuroprotective Effects via Anti-Oxidative Activity Sun, Yujuan Lin, Yanfei Cao, Xueli Xiang, Lan Qi, Jianhua Int J Mol Sci Article For screening anti-aging samples from marine natural products, K6001 yeast strain was employed as a bioassay system. The active mussel extract was separated to give an active sterol fraction (SF). SF was further purified, and four sterol compounds were obtained. Their structures were determined to be cholesterol (CHOL), brassicasterol, crinosterol, and 24-methylenecholesterol. All compounds showed similar anti-aging activity. To understand the action mechanism involved, anti-oxidative experiments, reactive oxygen species (ROS) assays, and malondialdehyde (MDA) tests were performed on the most abundant compound, CHOL. Results indicated that treatment with CHOL increases the survival rate of yeast under oxidative stress and decreases ROS and MDA levels. In addition, mutations of uth1, skn7, sod1, and sod2, which feature a K6001 background, were employed and the lifespans of the mutations were not affected by CHOL. These results demonstrate that CHOL exerts anti-aging effects via anti-oxidative stress. Based on the connection between neuroprotection and anti-aging, neuroprotective experiments were performed in PC12 cells. Paraquat was used to induce oxidative stress and the results showed that the CHOL and SF protect the PC12 cells from the injury induced by paraquat. In addition, these substance exhibited nerve growth factor (NGF) mimic activities again confirmed their neuroprotective function. MDPI 2014-11-25 /pmc/articles/PMC4284670/ /pubmed/25429428 http://dx.doi.org/10.3390/ijms151221660 Text en © 2014 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Sun, Yujuan Lin, Yanfei Cao, Xueli Xiang, Lan Qi, Jianhua Sterols from Mytilidae Show Anti-Aging and Neuroprotective Effects via Anti-Oxidative Activity |
title | Sterols from Mytilidae Show Anti-Aging and Neuroprotective Effects via Anti-Oxidative Activity |
title_full | Sterols from Mytilidae Show Anti-Aging and Neuroprotective Effects via Anti-Oxidative Activity |
title_fullStr | Sterols from Mytilidae Show Anti-Aging and Neuroprotective Effects via Anti-Oxidative Activity |
title_full_unstemmed | Sterols from Mytilidae Show Anti-Aging and Neuroprotective Effects via Anti-Oxidative Activity |
title_short | Sterols from Mytilidae Show Anti-Aging and Neuroprotective Effects via Anti-Oxidative Activity |
title_sort | sterols from mytilidae show anti-aging and neuroprotective effects via anti-oxidative activity |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4284670/ https://www.ncbi.nlm.nih.gov/pubmed/25429428 http://dx.doi.org/10.3390/ijms151221660 |
work_keys_str_mv | AT sunyujuan sterolsfrommytilidaeshowantiagingandneuroprotectiveeffectsviaantioxidativeactivity AT linyanfei sterolsfrommytilidaeshowantiagingandneuroprotectiveeffectsviaantioxidativeactivity AT caoxueli sterolsfrommytilidaeshowantiagingandneuroprotectiveeffectsviaantioxidativeactivity AT xianglan sterolsfrommytilidaeshowantiagingandneuroprotectiveeffectsviaantioxidativeactivity AT qijianhua sterolsfrommytilidaeshowantiagingandneuroprotectiveeffectsviaantioxidativeactivity |