Construction and Characterization of Three Wheat Bacterial Artificial Chromosome Libraries

We have constructed three bacterial artificial chromosome (BAC) libraries of wheat cultivar Triticum aestivum Wangshuibai, germplasms T. monococcum TA2026 and TA2033. A total of 1,233,792,170,880 and 263,040 clones were picked and arrayed in 384-well plates. On the basis of genome sizes of 16.8 Gb f...

Descripción completa

Detalles Bibliográficos
Autores principales: Cao, Wenjin, Fu, Bisheng, Wu, Kun, Li, Na, Zhou, Yan, Gao, Zhongxia, Lin, Musen, Li, Guoqiang, Wu,  Xinyi, Ma, Zhengqiang, Jia, Haiyan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2014
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4284684/
https://www.ncbi.nlm.nih.gov/pubmed/25464379
http://dx.doi.org/10.3390/ijms151221896
_version_ 1782351428852908032
author Cao, Wenjin
Fu, Bisheng
Wu, Kun
Li, Na
Zhou, Yan
Gao, Zhongxia
Lin, Musen
Li, Guoqiang
Wu,  Xinyi
Ma, Zhengqiang
Jia, Haiyan
author_facet Cao, Wenjin
Fu, Bisheng
Wu, Kun
Li, Na
Zhou, Yan
Gao, Zhongxia
Lin, Musen
Li, Guoqiang
Wu,  Xinyi
Ma, Zhengqiang
Jia, Haiyan
author_sort Cao, Wenjin
collection PubMed
description We have constructed three bacterial artificial chromosome (BAC) libraries of wheat cultivar Triticum aestivum Wangshuibai, germplasms T. monococcum TA2026 and TA2033. A total of 1,233,792,170,880 and 263,040 clones were picked and arrayed in 384-well plates. On the basis of genome sizes of 16.8 Gb for hexaploid wheat and 5.6 Gb for diploid wheat, the three libraries represented 9.05-, 2.60-, and 3.71-fold coverage of the haploid genomes, respectively. An improved descending pooling system for BAC libraries screening was established. This improved strategy can save 80% of the time and 68% of polymerase chain reaction (PCR) with the same successful rate as the universal 6D pooling strategy.
format Online
Article
Text
id pubmed-4284684
institution National Center for Biotechnology Information
language English
publishDate 2014
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-42846842015-01-21 Construction and Characterization of Three Wheat Bacterial Artificial Chromosome Libraries Cao, Wenjin Fu, Bisheng Wu, Kun Li, Na Zhou, Yan Gao, Zhongxia Lin, Musen Li, Guoqiang Wu,  Xinyi Ma, Zhengqiang Jia, Haiyan Int J Mol Sci Article We have constructed three bacterial artificial chromosome (BAC) libraries of wheat cultivar Triticum aestivum Wangshuibai, germplasms T. monococcum TA2026 and TA2033. A total of 1,233,792,170,880 and 263,040 clones were picked and arrayed in 384-well plates. On the basis of genome sizes of 16.8 Gb for hexaploid wheat and 5.6 Gb for diploid wheat, the three libraries represented 9.05-, 2.60-, and 3.71-fold coverage of the haploid genomes, respectively. An improved descending pooling system for BAC libraries screening was established. This improved strategy can save 80% of the time and 68% of polymerase chain reaction (PCR) with the same successful rate as the universal 6D pooling strategy. MDPI 2014-11-28 /pmc/articles/PMC4284684/ /pubmed/25464379 http://dx.doi.org/10.3390/ijms151221896 Text en © 2014 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Cao, Wenjin
Fu, Bisheng
Wu, Kun
Li, Na
Zhou, Yan
Gao, Zhongxia
Lin, Musen
Li, Guoqiang
Wu,  Xinyi
Ma, Zhengqiang
Jia, Haiyan
Construction and Characterization of Three Wheat Bacterial Artificial Chromosome Libraries
title Construction and Characterization of Three Wheat Bacterial Artificial Chromosome Libraries
title_full Construction and Characterization of Three Wheat Bacterial Artificial Chromosome Libraries
title_fullStr Construction and Characterization of Three Wheat Bacterial Artificial Chromosome Libraries
title_full_unstemmed Construction and Characterization of Three Wheat Bacterial Artificial Chromosome Libraries
title_short Construction and Characterization of Three Wheat Bacterial Artificial Chromosome Libraries
title_sort construction and characterization of three wheat bacterial artificial chromosome libraries
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4284684/
https://www.ncbi.nlm.nih.gov/pubmed/25464379
http://dx.doi.org/10.3390/ijms151221896
work_keys_str_mv AT caowenjin constructionandcharacterizationofthreewheatbacterialartificialchromosomelibraries
AT fubisheng constructionandcharacterizationofthreewheatbacterialartificialchromosomelibraries
AT wukun constructionandcharacterizationofthreewheatbacterialartificialchromosomelibraries
AT lina constructionandcharacterizationofthreewheatbacterialartificialchromosomelibraries
AT zhouyan constructionandcharacterizationofthreewheatbacterialartificialchromosomelibraries
AT gaozhongxia constructionandcharacterizationofthreewheatbacterialartificialchromosomelibraries
AT linmusen constructionandcharacterizationofthreewheatbacterialartificialchromosomelibraries
AT liguoqiang constructionandcharacterizationofthreewheatbacterialartificialchromosomelibraries
AT wuxinyi constructionandcharacterizationofthreewheatbacterialartificialchromosomelibraries
AT mazhengqiang constructionandcharacterizationofthreewheatbacterialartificialchromosomelibraries
AT jiahaiyan constructionandcharacterizationofthreewheatbacterialartificialchromosomelibraries

Ejemplares similares