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The Sesquiterpene Biosynthesis and Vessel-Occlusion Formation in Stems of Aquilaria sinensis (Lour.) Gilg Trees Induced by Wounding Treatments without Variation of Microbial Communities

As widely recognized, agarwood formation in Aquilaria trees is induced by external wounding. Because agarwood usually harbors specific microbes, the function of microbes in agarwood formation has been debated for almost a century. In this study, two wounding methods, the burning-chisel-drilling meth...

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Autores principales: Zhang, Zheng, Wei, Jianhe, Han, Xiaomin, Liang, Liang, Yang, Yun, Meng, Hui, Xu, Yanhong, Gao, Zhihui
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4284782/
https://www.ncbi.nlm.nih.gov/pubmed/25530613
http://dx.doi.org/10.3390/ijms151223589
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author Zhang, Zheng
Wei, Jianhe
Han, Xiaomin
Liang, Liang
Yang, Yun
Meng, Hui
Xu, Yanhong
Gao, Zhihui
author_facet Zhang, Zheng
Wei, Jianhe
Han, Xiaomin
Liang, Liang
Yang, Yun
Meng, Hui
Xu, Yanhong
Gao, Zhihui
author_sort Zhang, Zheng
collection PubMed
description As widely recognized, agarwood formation in Aquilaria trees is induced by external wounding. Because agarwood usually harbors specific microbes, the function of microbes in agarwood formation has been debated for almost a century. In this study, two wounding methods, the burning-chisel-drilling method (BCD) and the whole-tree agarwood-inducing method (Agar-Wit), were used under the non-contamination of environmental microorganisms. After pyrosequencing the small rRNA subunits of the wounds induced by the BCD and Agar-Wit, no substantial variation was observed either in fungal and bacterial enrichment and diversity or in the relative abundances of taxa. By contrast, significant variations in fungal and bacterial communities were detected following the partial tree pruning (PTP)-wounding. The wound-induced sesquiterpene biosynthesis and vessel-occlusion formation, however, were found to be similar in all types of wounded trunks. We thus infer that wounding in the absence of variations in microbial communities may induce agarwood formation. This result does not support the long-standing notion that agarwood formation depends on microbes.
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spelling pubmed-42847822015-01-21 The Sesquiterpene Biosynthesis and Vessel-Occlusion Formation in Stems of Aquilaria sinensis (Lour.) Gilg Trees Induced by Wounding Treatments without Variation of Microbial Communities Zhang, Zheng Wei, Jianhe Han, Xiaomin Liang, Liang Yang, Yun Meng, Hui Xu, Yanhong Gao, Zhihui Int J Mol Sci Article As widely recognized, agarwood formation in Aquilaria trees is induced by external wounding. Because agarwood usually harbors specific microbes, the function of microbes in agarwood formation has been debated for almost a century. In this study, two wounding methods, the burning-chisel-drilling method (BCD) and the whole-tree agarwood-inducing method (Agar-Wit), were used under the non-contamination of environmental microorganisms. After pyrosequencing the small rRNA subunits of the wounds induced by the BCD and Agar-Wit, no substantial variation was observed either in fungal and bacterial enrichment and diversity or in the relative abundances of taxa. By contrast, significant variations in fungal and bacterial communities were detected following the partial tree pruning (PTP)-wounding. The wound-induced sesquiterpene biosynthesis and vessel-occlusion formation, however, were found to be similar in all types of wounded trunks. We thus infer that wounding in the absence of variations in microbial communities may induce agarwood formation. This result does not support the long-standing notion that agarwood formation depends on microbes. MDPI 2014-12-18 /pmc/articles/PMC4284782/ /pubmed/25530613 http://dx.doi.org/10.3390/ijms151223589 Text en © 2014 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Zhang, Zheng
Wei, Jianhe
Han, Xiaomin
Liang, Liang
Yang, Yun
Meng, Hui
Xu, Yanhong
Gao, Zhihui
The Sesquiterpene Biosynthesis and Vessel-Occlusion Formation in Stems of Aquilaria sinensis (Lour.) Gilg Trees Induced by Wounding Treatments without Variation of Microbial Communities
title The Sesquiterpene Biosynthesis and Vessel-Occlusion Formation in Stems of Aquilaria sinensis (Lour.) Gilg Trees Induced by Wounding Treatments without Variation of Microbial Communities
title_full The Sesquiterpene Biosynthesis and Vessel-Occlusion Formation in Stems of Aquilaria sinensis (Lour.) Gilg Trees Induced by Wounding Treatments without Variation of Microbial Communities
title_fullStr The Sesquiterpene Biosynthesis and Vessel-Occlusion Formation in Stems of Aquilaria sinensis (Lour.) Gilg Trees Induced by Wounding Treatments without Variation of Microbial Communities
title_full_unstemmed The Sesquiterpene Biosynthesis and Vessel-Occlusion Formation in Stems of Aquilaria sinensis (Lour.) Gilg Trees Induced by Wounding Treatments without Variation of Microbial Communities
title_short The Sesquiterpene Biosynthesis and Vessel-Occlusion Formation in Stems of Aquilaria sinensis (Lour.) Gilg Trees Induced by Wounding Treatments without Variation of Microbial Communities
title_sort sesquiterpene biosynthesis and vessel-occlusion formation in stems of aquilaria sinensis (lour.) gilg trees induced by wounding treatments without variation of microbial communities
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4284782/
https://www.ncbi.nlm.nih.gov/pubmed/25530613
http://dx.doi.org/10.3390/ijms151223589
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