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Nano-Scale Spatial Assessment of Calcium Distribution in Coccolithophores Using Synchrotron-Based Nano-CT and STXM-NEXAFS

Calcified coccolithophores generate calcium carbonate scales around their cell surface. In light of predicted climate change and the global carbon cycle, the biomineralization ability of coccoliths has received growing interest. However, the underlying biomineralization mechanism is not yet well und...

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Autores principales: Sun, Shiyong, Yao, Yanchen, Zou, Xiang, Fan, Shenglan, Zhou, Qing, Dai, Qunwei, Dong, Faqin, Liu, Mingxue, Nie, Xiaoqin, Tan, Daoyong, Li, Shuai
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4284783/
https://www.ncbi.nlm.nih.gov/pubmed/25530614
http://dx.doi.org/10.3390/ijms151223604
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author Sun, Shiyong
Yao, Yanchen
Zou, Xiang
Fan, Shenglan
Zhou, Qing
Dai, Qunwei
Dong, Faqin
Liu, Mingxue
Nie, Xiaoqin
Tan, Daoyong
Li, Shuai
author_facet Sun, Shiyong
Yao, Yanchen
Zou, Xiang
Fan, Shenglan
Zhou, Qing
Dai, Qunwei
Dong, Faqin
Liu, Mingxue
Nie, Xiaoqin
Tan, Daoyong
Li, Shuai
author_sort Sun, Shiyong
collection PubMed
description Calcified coccolithophores generate calcium carbonate scales around their cell surface. In light of predicted climate change and the global carbon cycle, the biomineralization ability of coccoliths has received growing interest. However, the underlying biomineralization mechanism is not yet well understood; the lack of non-invasive characterizing tools to obtain molecular level information involving biogenic processes and biomineral components remain significant challenges. In the present study, synchrotron-based Nano-computed Tomography (Nano-CT) and Scanning Transmission X-ray Microscopy-Near-edge X-ray Absorption Fine Structure Spectromicroscopy (STXM-NEXAFS) techniques were employed to identify Ca spatial distribution and investigate the compositional chemistry and distinctive features of the association between biomacromolecules and mineral components of calcite present in coccoliths. The Nano-CT results show that the coccolith scale vesicle is similar as a continuous single channel. The mature coccoliths were intracellularly distributed and immediately ejected and located at the exterior surface to form a coccoshpere. The NEXAFS spectromicroscopy results of the Ca L edge clearly demonstrate the existence of two levels of gradients spatially, indicating two distinctive forms of Ca in coccoliths: a crystalline-poor layer surrounded by a relatively crystalline-rich layer. The results show that Sr is absorbed by the coccoliths and that Sr/Ca substitution is rather homogeneous within the coccoliths. Our findings indicate that synchrotron-based STXM-NEXAFS and Nano-CT are excellent tools for the study of biominerals and provide information to clarify biomineralization mechanism.
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spelling pubmed-42847832015-01-21 Nano-Scale Spatial Assessment of Calcium Distribution in Coccolithophores Using Synchrotron-Based Nano-CT and STXM-NEXAFS Sun, Shiyong Yao, Yanchen Zou, Xiang Fan, Shenglan Zhou, Qing Dai, Qunwei Dong, Faqin Liu, Mingxue Nie, Xiaoqin Tan, Daoyong Li, Shuai Int J Mol Sci Article Calcified coccolithophores generate calcium carbonate scales around their cell surface. In light of predicted climate change and the global carbon cycle, the biomineralization ability of coccoliths has received growing interest. However, the underlying biomineralization mechanism is not yet well understood; the lack of non-invasive characterizing tools to obtain molecular level information involving biogenic processes and biomineral components remain significant challenges. In the present study, synchrotron-based Nano-computed Tomography (Nano-CT) and Scanning Transmission X-ray Microscopy-Near-edge X-ray Absorption Fine Structure Spectromicroscopy (STXM-NEXAFS) techniques were employed to identify Ca spatial distribution and investigate the compositional chemistry and distinctive features of the association between biomacromolecules and mineral components of calcite present in coccoliths. The Nano-CT results show that the coccolith scale vesicle is similar as a continuous single channel. The mature coccoliths were intracellularly distributed and immediately ejected and located at the exterior surface to form a coccoshpere. The NEXAFS spectromicroscopy results of the Ca L edge clearly demonstrate the existence of two levels of gradients spatially, indicating two distinctive forms of Ca in coccoliths: a crystalline-poor layer surrounded by a relatively crystalline-rich layer. The results show that Sr is absorbed by the coccoliths and that Sr/Ca substitution is rather homogeneous within the coccoliths. Our findings indicate that synchrotron-based STXM-NEXAFS and Nano-CT are excellent tools for the study of biominerals and provide information to clarify biomineralization mechanism. MDPI 2014-12-18 /pmc/articles/PMC4284783/ /pubmed/25530614 http://dx.doi.org/10.3390/ijms151223604 Text en © 2014 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Sun, Shiyong
Yao, Yanchen
Zou, Xiang
Fan, Shenglan
Zhou, Qing
Dai, Qunwei
Dong, Faqin
Liu, Mingxue
Nie, Xiaoqin
Tan, Daoyong
Li, Shuai
Nano-Scale Spatial Assessment of Calcium Distribution in Coccolithophores Using Synchrotron-Based Nano-CT and STXM-NEXAFS
title Nano-Scale Spatial Assessment of Calcium Distribution in Coccolithophores Using Synchrotron-Based Nano-CT and STXM-NEXAFS
title_full Nano-Scale Spatial Assessment of Calcium Distribution in Coccolithophores Using Synchrotron-Based Nano-CT and STXM-NEXAFS
title_fullStr Nano-Scale Spatial Assessment of Calcium Distribution in Coccolithophores Using Synchrotron-Based Nano-CT and STXM-NEXAFS
title_full_unstemmed Nano-Scale Spatial Assessment of Calcium Distribution in Coccolithophores Using Synchrotron-Based Nano-CT and STXM-NEXAFS
title_short Nano-Scale Spatial Assessment of Calcium Distribution in Coccolithophores Using Synchrotron-Based Nano-CT and STXM-NEXAFS
title_sort nano-scale spatial assessment of calcium distribution in coccolithophores using synchrotron-based nano-ct and stxm-nexafs
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4284783/
https://www.ncbi.nlm.nih.gov/pubmed/25530614
http://dx.doi.org/10.3390/ijms151223604
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