Candida parapsilosis Biofilm Identification by Raman Spectroscopy

Colonies of Candida parapsilosis on culture plates were probed directly in situ using Raman spectroscopy for rapid identification of specific strains separated by a given time intervals (up to months apart). To classify the Raman spectra, data analysis was performed using the approach of principal c...

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Autores principales: Samek, Ota, Mlynariková, Katarina, Bernatová, Silvie, Ježek, Jan, Krzyžánek, Vladislav, Šiler, Martin, Zemánek, Pavel, Růžička, Filip, Holá, Veronika, Mahelová, Martina
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2014
Materias:
Short Note
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4284798/
https://www.ncbi.nlm.nih.gov/pubmed/25535081
http://dx.doi.org/10.3390/ijms151223924
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author Samek, Ota
Mlynariková, Katarina
Bernatová, Silvie
Ježek, Jan
Krzyžánek, Vladislav
Šiler, Martin
Zemánek, Pavel
Růžička, Filip
Holá, Veronika
Mahelová, Martina
author_facet Samek, Ota
Mlynariková, Katarina
Bernatová, Silvie
Ježek, Jan
Krzyžánek, Vladislav
Šiler, Martin
Zemánek, Pavel
Růžička, Filip
Holá, Veronika
Mahelová, Martina
author_sort Samek, Ota
collection PubMed
description Colonies of Candida parapsilosis on culture plates were probed directly in situ using Raman spectroscopy for rapid identification of specific strains separated by a given time intervals (up to months apart). To classify the Raman spectra, data analysis was performed using the approach of principal component analysis (PCA). The analysis of the data sets generated during the scans of individual colonies reveals that despite the inhomogeneity of the biological samples unambiguous associations to individual strains (two biofilm-positive and two biofilm-negative) could be made.
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spelling pubmed-42847982015-01-21 Candida parapsilosis Biofilm Identification by Raman Spectroscopy Samek, Ota Mlynariková, Katarina Bernatová, Silvie Ježek, Jan Krzyžánek, Vladislav Šiler, Martin Zemánek, Pavel Růžička, Filip Holá, Veronika Mahelová, Martina Int J Mol Sci Short Note Colonies of Candida parapsilosis on culture plates were probed directly in situ using Raman spectroscopy for rapid identification of specific strains separated by a given time intervals (up to months apart). To classify the Raman spectra, data analysis was performed using the approach of principal component analysis (PCA). The analysis of the data sets generated during the scans of individual colonies reveals that despite the inhomogeneity of the biological samples unambiguous associations to individual strains (two biofilm-positive and two biofilm-negative) could be made. MDPI 2014-12-22 /pmc/articles/PMC4284798/ /pubmed/25535081 http://dx.doi.org/10.3390/ijms151223924 Text en © 2014 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Short Note
Samek, Ota
Mlynariková, Katarina
Bernatová, Silvie
Ježek, Jan
Krzyžánek, Vladislav
Šiler, Martin
Zemánek, Pavel
Růžička, Filip
Holá, Veronika
Mahelová, Martina
Candida parapsilosis Biofilm Identification by Raman Spectroscopy
title Candida parapsilosis Biofilm Identification by Raman Spectroscopy
title_full Candida parapsilosis Biofilm Identification by Raman Spectroscopy
title_fullStr Candida parapsilosis Biofilm Identification by Raman Spectroscopy
title_full_unstemmed Candida parapsilosis Biofilm Identification by Raman Spectroscopy
title_short Candida parapsilosis Biofilm Identification by Raman Spectroscopy
title_sort candida parapsilosis biofilm identification by raman spectroscopy
topic Short Note
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4284798/
https://www.ncbi.nlm.nih.gov/pubmed/25535081
http://dx.doi.org/10.3390/ijms151223924
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