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Targeting the endoplasmic reticulum with a membrane-interactive luminescent ruthenium(ii) polypyridyl complex

The characterization and bioactivity of the dinuclear ruthenium(ii) complex [(Ru(DIP)(2))(2)(tpphz)](4+) (DIP = 4,7-diphenyl-1,10-phenanthroline and tpphz = tetrapyrido[3,2-a:2′,3′-c:3′′,2′′-h:2′′′,3′′′-j]phenazine) is reported. This new complex is found to be luminescent in acetonitrile, where exci...

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Autores principales: Gill, Martin R., Cecchin, Denis, Walker, Michael G., Mulla, Raminder S., Battaglia, Giuseppe, Smythe, Carl, Thomas, Jim A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Royal Society of Chemistry 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4285105/
https://www.ncbi.nlm.nih.gov/pubmed/25580209
http://dx.doi.org/10.1039/c3sc51725j
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author Gill, Martin R.
Cecchin, Denis
Walker, Michael G.
Mulla, Raminder S.
Battaglia, Giuseppe
Smythe, Carl
Thomas, Jim A.
author_facet Gill, Martin R.
Cecchin, Denis
Walker, Michael G.
Mulla, Raminder S.
Battaglia, Giuseppe
Smythe, Carl
Thomas, Jim A.
author_sort Gill, Martin R.
collection PubMed
description The characterization and bioactivity of the dinuclear ruthenium(ii) complex [(Ru(DIP)(2))(2)(tpphz)](4+) (DIP = 4,7-diphenyl-1,10-phenanthroline and tpphz = tetrapyrido[3,2-a:2′,3′-c:3′′,2′′-h:2′′′,3′′′-j]phenazine) is reported. This new complex is found to be luminescent in acetonitrile, where excitation into MLCT (metal-to-ligand charge-transfer) bands in the visible area of the spectrum (λ (ex) = 450 nm, ε = 45 000 M(–1) cm(–1)) result in red emission (λ (em,max) = 620 nm, Φ (MLCT) = 0.017). Aqueous in vitro binding studies indicate that this complex binds to duplex DNA with an affinity of 1.8 × 10(6) M(–1) through a non-classical groove-binding interaction, however, unlike the parent complex [(Ru(phen)(2))(2)(tpphz)](4+) (phen = 1,10-phenanthroline), it also displays an increase in MLCT luminescence on addition of liposomes. Confocal microscopy and TEM studies show that this lipophilic complex targets the endoplasmic reticulum of eukaryotic cells, where it functions as an imaging agent for this organelle, and cytotoxicity studies in human cancer cell lines indicate a comparable potency to the anti-cancer drug cisplatin.
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spelling pubmed-42851052015-01-08 Targeting the endoplasmic reticulum with a membrane-interactive luminescent ruthenium(ii) polypyridyl complex Gill, Martin R. Cecchin, Denis Walker, Michael G. Mulla, Raminder S. Battaglia, Giuseppe Smythe, Carl Thomas, Jim A. Chem Sci Chemistry The characterization and bioactivity of the dinuclear ruthenium(ii) complex [(Ru(DIP)(2))(2)(tpphz)](4+) (DIP = 4,7-diphenyl-1,10-phenanthroline and tpphz = tetrapyrido[3,2-a:2′,3′-c:3′′,2′′-h:2′′′,3′′′-j]phenazine) is reported. This new complex is found to be luminescent in acetonitrile, where excitation into MLCT (metal-to-ligand charge-transfer) bands in the visible area of the spectrum (λ (ex) = 450 nm, ε = 45 000 M(–1) cm(–1)) result in red emission (λ (em,max) = 620 nm, Φ (MLCT) = 0.017). Aqueous in vitro binding studies indicate that this complex binds to duplex DNA with an affinity of 1.8 × 10(6) M(–1) through a non-classical groove-binding interaction, however, unlike the parent complex [(Ru(phen)(2))(2)(tpphz)](4+) (phen = 1,10-phenanthroline), it also displays an increase in MLCT luminescence on addition of liposomes. Confocal microscopy and TEM studies show that this lipophilic complex targets the endoplasmic reticulum of eukaryotic cells, where it functions as an imaging agent for this organelle, and cytotoxicity studies in human cancer cell lines indicate a comparable potency to the anti-cancer drug cisplatin. Royal Society of Chemistry 2013-12-28 2013-10-16 /pmc/articles/PMC4285105/ /pubmed/25580209 http://dx.doi.org/10.1039/c3sc51725j Text en This journal is © The Royal Society of Chemistry 2013 http://creativecommons.org/licenses/by/3.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution 3.0 Unported License (http://creativecommons.org/licenses/by/3.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Chemistry
Gill, Martin R.
Cecchin, Denis
Walker, Michael G.
Mulla, Raminder S.
Battaglia, Giuseppe
Smythe, Carl
Thomas, Jim A.
Targeting the endoplasmic reticulum with a membrane-interactive luminescent ruthenium(ii) polypyridyl complex
title Targeting the endoplasmic reticulum with a membrane-interactive luminescent ruthenium(ii) polypyridyl complex
title_full Targeting the endoplasmic reticulum with a membrane-interactive luminescent ruthenium(ii) polypyridyl complex
title_fullStr Targeting the endoplasmic reticulum with a membrane-interactive luminescent ruthenium(ii) polypyridyl complex
title_full_unstemmed Targeting the endoplasmic reticulum with a membrane-interactive luminescent ruthenium(ii) polypyridyl complex
title_short Targeting the endoplasmic reticulum with a membrane-interactive luminescent ruthenium(ii) polypyridyl complex
title_sort targeting the endoplasmic reticulum with a membrane-interactive luminescent ruthenium(ii) polypyridyl complex
topic Chemistry
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4285105/
https://www.ncbi.nlm.nih.gov/pubmed/25580209
http://dx.doi.org/10.1039/c3sc51725j
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