Click chemistry facilitates direct labelling and super-resolution imaging of nucleic acids and proteins

We demonstrate high-density labelling of cellular DNA and RNA using click chemistry and perform confocal and super-resolution microscopy. We visualize the crescent and ring-like structure of densely packed RNA in nucleoli. We further demonstrate click chemistry with unnatural amino acids for super-r...

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Detalles Bibliográficos
Autores principales: Raulf, Anika, Spahn, Christoph K., Zessin, Patrick J. M., Finan, Kieran, Bernhardt, Stefan, Heckel, Alexander, Heilemann, Mike
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Royal Society of Chemistry 2014
Materias:
Chemistry
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4285124/
https://www.ncbi.nlm.nih.gov/pubmed/25580242
http://dx.doi.org/10.1039/c4ra01027b
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author Raulf, Anika
Spahn, Christoph K.
Zessin, Patrick J. M.
Finan, Kieran
Bernhardt, Stefan
Heckel, Alexander
Heilemann, Mike
author_facet Raulf, Anika
Spahn, Christoph K.
Zessin, Patrick J. M.
Finan, Kieran
Bernhardt, Stefan
Heckel, Alexander
Heilemann, Mike
author_sort Raulf, Anika
collection PubMed
description We demonstrate high-density labelling of cellular DNA and RNA using click chemistry and perform confocal and super-resolution microscopy. We visualize the crescent and ring-like structure of densely packed RNA in nucleoli. We further demonstrate click chemistry with unnatural amino acids for super-resolution imaging of outer-membrane proteins of E. coli.
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spelling pubmed-42851242015-01-08 Click chemistry facilitates direct labelling and super-resolution imaging of nucleic acids and proteins Raulf, Anika Spahn, Christoph K. Zessin, Patrick J. M. Finan, Kieran Bernhardt, Stefan Heckel, Alexander Heilemann, Mike RSC Adv Chemistry We demonstrate high-density labelling of cellular DNA and RNA using click chemistry and perform confocal and super-resolution microscopy. We visualize the crescent and ring-like structure of densely packed RNA in nucleoli. We further demonstrate click chemistry with unnatural amino acids for super-resolution imaging of outer-membrane proteins of E. coli. Royal Society of Chemistry 2014-07-16 2014-12-24 /pmc/articles/PMC4285124/ /pubmed/25580242 http://dx.doi.org/10.1039/c4ra01027b Text en This journal is © The Royal Society of Chemistry 2014 http://creativecommons.org/licenses/by/3.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution 3.0 Unported License (http://creativecommons.org/licenses/by/3.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Chemistry
Raulf, Anika
Spahn, Christoph K.
Zessin, Patrick J. M.
Finan, Kieran
Bernhardt, Stefan
Heckel, Alexander
Heilemann, Mike
Click chemistry facilitates direct labelling and super-resolution imaging of nucleic acids and proteins
title Click chemistry facilitates direct labelling and super-resolution imaging of nucleic acids and proteins
title_full Click chemistry facilitates direct labelling and super-resolution imaging of nucleic acids and proteins
title_fullStr Click chemistry facilitates direct labelling and super-resolution imaging of nucleic acids and proteins
title_full_unstemmed Click chemistry facilitates direct labelling and super-resolution imaging of nucleic acids and proteins
title_short Click chemistry facilitates direct labelling and super-resolution imaging of nucleic acids and proteins
title_sort click chemistry facilitates direct labelling and super-resolution imaging of nucleic acids and proteins
topic Chemistry
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4285124/
https://www.ncbi.nlm.nih.gov/pubmed/25580242
http://dx.doi.org/10.1039/c4ra01027b
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