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Microbiology of diabetic foot infections: from Louis Pasteur to ‘crime scene investigation’

Were he alive today, would Louis Pasteur still champion culture methods he pioneered over 150 years ago for identifying bacterial pathogens? Or, might he suggest that new molecular techniques may prove a better way forward for quickly detecting the true microbial diversity of wounds? As modern clini...

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Detalles Bibliográficos
Autores principales: Spichler, Anne, Hurwitz, Bonnie L, Armstrong, David G, Lipsky, Benjamin A
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4286146/
https://www.ncbi.nlm.nih.gov/pubmed/25564342
http://dx.doi.org/10.1186/s12916-014-0232-0
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author Spichler, Anne
Hurwitz, Bonnie L
Armstrong, David G
Lipsky, Benjamin A
author_facet Spichler, Anne
Hurwitz, Bonnie L
Armstrong, David G
Lipsky, Benjamin A
author_sort Spichler, Anne
collection PubMed
description Were he alive today, would Louis Pasteur still champion culture methods he pioneered over 150 years ago for identifying bacterial pathogens? Or, might he suggest that new molecular techniques may prove a better way forward for quickly detecting the true microbial diversity of wounds? As modern clinicians faced with treating complex patients with diabetic foot infections (DFI), should we still request venerated and familiar culture and sensitivity methods, or is it time to ask for newer molecular tests, such as 16S rRNA gene sequencing? Or, are molecular techniques as yet too experimental, non-specific and expensive for current clinical use? While molecular techniques help us to identify more microorganisms from a DFI, can they tell us ‘who done it?’, that is, which are the causative pathogens and which are merely colonizers? Furthermore, can molecular techniques provide clinically relevant, rapid information on the virulence of wound isolates and their antibiotic sensitivities? We herein review current knowledge on the microbiology of DFI, from standard culture methods to the current era of rapid and comprehensive ‘crime scene investigation’ (CSI) techniques.
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spelling pubmed-42861462015-01-08 Microbiology of diabetic foot infections: from Louis Pasteur to ‘crime scene investigation’ Spichler, Anne Hurwitz, Bonnie L Armstrong, David G Lipsky, Benjamin A BMC Med Review Were he alive today, would Louis Pasteur still champion culture methods he pioneered over 150 years ago for identifying bacterial pathogens? Or, might he suggest that new molecular techniques may prove a better way forward for quickly detecting the true microbial diversity of wounds? As modern clinicians faced with treating complex patients with diabetic foot infections (DFI), should we still request venerated and familiar culture and sensitivity methods, or is it time to ask for newer molecular tests, such as 16S rRNA gene sequencing? Or, are molecular techniques as yet too experimental, non-specific and expensive for current clinical use? While molecular techniques help us to identify more microorganisms from a DFI, can they tell us ‘who done it?’, that is, which are the causative pathogens and which are merely colonizers? Furthermore, can molecular techniques provide clinically relevant, rapid information on the virulence of wound isolates and their antibiotic sensitivities? We herein review current knowledge on the microbiology of DFI, from standard culture methods to the current era of rapid and comprehensive ‘crime scene investigation’ (CSI) techniques. BioMed Central 2015-01-07 /pmc/articles/PMC4286146/ /pubmed/25564342 http://dx.doi.org/10.1186/s12916-014-0232-0 Text en © Spichler et al.; licensee BioMed Central. 2015 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Review
Spichler, Anne
Hurwitz, Bonnie L
Armstrong, David G
Lipsky, Benjamin A
Microbiology of diabetic foot infections: from Louis Pasteur to ‘crime scene investigation’
title Microbiology of diabetic foot infections: from Louis Pasteur to ‘crime scene investigation’
title_full Microbiology of diabetic foot infections: from Louis Pasteur to ‘crime scene investigation’
title_fullStr Microbiology of diabetic foot infections: from Louis Pasteur to ‘crime scene investigation’
title_full_unstemmed Microbiology of diabetic foot infections: from Louis Pasteur to ‘crime scene investigation’
title_short Microbiology of diabetic foot infections: from Louis Pasteur to ‘crime scene investigation’
title_sort microbiology of diabetic foot infections: from louis pasteur to ‘crime scene investigation’
topic Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4286146/
https://www.ncbi.nlm.nih.gov/pubmed/25564342
http://dx.doi.org/10.1186/s12916-014-0232-0
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