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Human Milk Glycomics and Gut Microbial Genomics in Infant Feces Show a Correlation between Human Milk Oligosaccharides and Gut Microbiota: A Proof-of-Concept Study
[Image: see text] Human milk oligosaccharides (HMOs) play a key role in shaping and maintaining a healthy infant gut microbiota. This article demonstrates the potential of combining recent advances in glycomics and genomics to correlate abundances of fecal microbes and fecal HMOs. Serial fecal speci...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
American Chemical
Society
2014
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4286166/ https://www.ncbi.nlm.nih.gov/pubmed/25300177 http://dx.doi.org/10.1021/pr500759e |
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author | De Leoz, Maria Lorna A. Kalanetra, Karen M. Bokulich, Nicholas A. Strum, John S. Underwood, Mark A. German, J. Bruce Mills, David A. Lebrilla, Carlito B. |
author_facet | De Leoz, Maria Lorna A. Kalanetra, Karen M. Bokulich, Nicholas A. Strum, John S. Underwood, Mark A. German, J. Bruce Mills, David A. Lebrilla, Carlito B. |
author_sort | De Leoz, Maria Lorna A. |
collection | PubMed |
description | [Image: see text] Human milk oligosaccharides (HMOs) play a key role in shaping and maintaining a healthy infant gut microbiota. This article demonstrates the potential of combining recent advances in glycomics and genomics to correlate abundances of fecal microbes and fecal HMOs. Serial fecal specimens from two healthy breast-fed infants were analyzed by bacterial DNA sequencing to characterize the microbiota and by mass spectrometry to determine abundances of specific HMOs that passed through the intestinal tract without being consumed by the luminal bacteria. In both infants, the fecal bacterial population shifted from non-HMO-consuming microbes to HMO-consuming bacteria during the first few weeks of life. An initial rise in fecal HMOs corresponded with bacterial populations composed primarily of non-HMO-consuming Enterobacteriaceae and Staphylococcaeae. This was followed by decreases in fecal HMOs as the proportion of HMO-consuming Bacteroidaceae and Bifidobacteriaceae increased. Analysis of HMO structures with isomer differentiation revealed that HMO consumption is highly structure-specific, with unique isomers being consumed and others passing through the gut unaltered. These results represent a proof-of-concept and are consistent with the highly selective, prebiotic effect of HMOs in shaping the gut microbiota in the first weeks of life. The analysis of selective fecal bacterial substrates as a measure of alterations in the gut microbiota may be a potential marker of dysbiosis. |
format | Online Article Text |
id | pubmed-4286166 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | American Chemical
Society |
record_format | MEDLINE/PubMed |
spelling | pubmed-42861662015-10-10 Human Milk Glycomics and Gut Microbial Genomics in Infant Feces Show a Correlation between Human Milk Oligosaccharides and Gut Microbiota: A Proof-of-Concept Study De Leoz, Maria Lorna A. Kalanetra, Karen M. Bokulich, Nicholas A. Strum, John S. Underwood, Mark A. German, J. Bruce Mills, David A. Lebrilla, Carlito B. J Proteome Res [Image: see text] Human milk oligosaccharides (HMOs) play a key role in shaping and maintaining a healthy infant gut microbiota. This article demonstrates the potential of combining recent advances in glycomics and genomics to correlate abundances of fecal microbes and fecal HMOs. Serial fecal specimens from two healthy breast-fed infants were analyzed by bacterial DNA sequencing to characterize the microbiota and by mass spectrometry to determine abundances of specific HMOs that passed through the intestinal tract without being consumed by the luminal bacteria. In both infants, the fecal bacterial population shifted from non-HMO-consuming microbes to HMO-consuming bacteria during the first few weeks of life. An initial rise in fecal HMOs corresponded with bacterial populations composed primarily of non-HMO-consuming Enterobacteriaceae and Staphylococcaeae. This was followed by decreases in fecal HMOs as the proportion of HMO-consuming Bacteroidaceae and Bifidobacteriaceae increased. Analysis of HMO structures with isomer differentiation revealed that HMO consumption is highly structure-specific, with unique isomers being consumed and others passing through the gut unaltered. These results represent a proof-of-concept and are consistent with the highly selective, prebiotic effect of HMOs in shaping the gut microbiota in the first weeks of life. The analysis of selective fecal bacterial substrates as a measure of alterations in the gut microbiota may be a potential marker of dysbiosis. American Chemical Society 2014-10-10 2015-01-02 /pmc/articles/PMC4286166/ /pubmed/25300177 http://dx.doi.org/10.1021/pr500759e Text en Copyright © 2014 American Chemical Society This is an open access article published under an ACS AuthorChoice License (http://pubs.acs.org/page/policy/authorchoice_termsofuse.html) , which permits copying and redistribution of the article or any adaptations for non-commercial purposes. |
spellingShingle | De Leoz, Maria Lorna A. Kalanetra, Karen M. Bokulich, Nicholas A. Strum, John S. Underwood, Mark A. German, J. Bruce Mills, David A. Lebrilla, Carlito B. Human Milk Glycomics and Gut Microbial Genomics in Infant Feces Show a Correlation between Human Milk Oligosaccharides and Gut Microbiota: A Proof-of-Concept Study |
title | Human Milk Glycomics and Gut
Microbial Genomics in
Infant Feces Show a Correlation between Human Milk Oligosaccharides
and Gut Microbiota: A Proof-of-Concept Study |
title_full | Human Milk Glycomics and Gut
Microbial Genomics in
Infant Feces Show a Correlation between Human Milk Oligosaccharides
and Gut Microbiota: A Proof-of-Concept Study |
title_fullStr | Human Milk Glycomics and Gut
Microbial Genomics in
Infant Feces Show a Correlation between Human Milk Oligosaccharides
and Gut Microbiota: A Proof-of-Concept Study |
title_full_unstemmed | Human Milk Glycomics and Gut
Microbial Genomics in
Infant Feces Show a Correlation between Human Milk Oligosaccharides
and Gut Microbiota: A Proof-of-Concept Study |
title_short | Human Milk Glycomics and Gut
Microbial Genomics in
Infant Feces Show a Correlation between Human Milk Oligosaccharides
and Gut Microbiota: A Proof-of-Concept Study |
title_sort | human milk glycomics and gut
microbial genomics in
infant feces show a correlation between human milk oligosaccharides
and gut microbiota: a proof-of-concept study |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4286166/ https://www.ncbi.nlm.nih.gov/pubmed/25300177 http://dx.doi.org/10.1021/pr500759e |
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