The multispecific thyroid hormone transporter OATP1C1 mediates cell-specific sulforhodamine 101-labeling of hippocampal astrocytes

Sulforhodamine 101 (SR101) is widely used for astrocyte identification, though the labeling mechanism remains unknown and the efficacy of labeling in different brain regions is heterogeneous. By combining region-specific isolation of astrocytes followed by transcriptome analysis, two-photon excitati...

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Autores principales: Schnell, Christian, Shahmoradi, Ali, Wichert, Sven P., Mayerl, Steffen, Hagos, Yohannes, Heuer, Heike, Rossner, Moritz J., Hülsmann, Swen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Berlin Heidelberg 2013
Materias:
Original Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4286632/
https://www.ncbi.nlm.nih.gov/pubmed/24129767
http://dx.doi.org/10.1007/s00429-013-0645-0
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author Schnell, Christian
Shahmoradi, Ali
Wichert, Sven P.
Mayerl, Steffen
Hagos, Yohannes
Heuer, Heike
Rossner, Moritz J.
Hülsmann, Swen
author_facet Schnell, Christian
Shahmoradi, Ali
Wichert, Sven P.
Mayerl, Steffen
Hagos, Yohannes
Heuer, Heike
Rossner, Moritz J.
Hülsmann, Swen
author_sort Schnell, Christian
collection PubMed
description Sulforhodamine 101 (SR101) is widely used for astrocyte identification, though the labeling mechanism remains unknown and the efficacy of labeling in different brain regions is heterogeneous. By combining region-specific isolation of astrocytes followed by transcriptome analysis, two-photon excitation microscopy, and mouse genetics, we identified the thyroid hormone transporter OATP1C1 as the SR101-uptake transporter in hippocampus and cortex. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1007/s00429-013-0645-0) contains supplementary material, which is available to authorized users.
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spelling pubmed-42866322015-01-15 The multispecific thyroid hormone transporter OATP1C1 mediates cell-specific sulforhodamine 101-labeling of hippocampal astrocytes Schnell, Christian Shahmoradi, Ali Wichert, Sven P. Mayerl, Steffen Hagos, Yohannes Heuer, Heike Rossner, Moritz J. Hülsmann, Swen Brain Struct Funct Original Article Sulforhodamine 101 (SR101) is widely used for astrocyte identification, though the labeling mechanism remains unknown and the efficacy of labeling in different brain regions is heterogeneous. By combining region-specific isolation of astrocytes followed by transcriptome analysis, two-photon excitation microscopy, and mouse genetics, we identified the thyroid hormone transporter OATP1C1 as the SR101-uptake transporter in hippocampus and cortex. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1007/s00429-013-0645-0) contains supplementary material, which is available to authorized users. Springer Berlin Heidelberg 2013-10-16 2015 /pmc/articles/PMC4286632/ /pubmed/24129767 http://dx.doi.org/10.1007/s00429-013-0645-0 Text en © The Author(s) 2013 https://creativecommons.org/licenses/by/2.0/ Open AccessThis article is distributed under the terms of the Creative Commons Attribution License which permits any use, distribution, and reproduction in any medium, provided the original author(s) and the source are credited.
spellingShingle Original Article
Schnell, Christian
Shahmoradi, Ali
Wichert, Sven P.
Mayerl, Steffen
Hagos, Yohannes
Heuer, Heike
Rossner, Moritz J.
Hülsmann, Swen
The multispecific thyroid hormone transporter OATP1C1 mediates cell-specific sulforhodamine 101-labeling of hippocampal astrocytes
title The multispecific thyroid hormone transporter OATP1C1 mediates cell-specific sulforhodamine 101-labeling of hippocampal astrocytes
title_full The multispecific thyroid hormone transporter OATP1C1 mediates cell-specific sulforhodamine 101-labeling of hippocampal astrocytes
title_fullStr The multispecific thyroid hormone transporter OATP1C1 mediates cell-specific sulforhodamine 101-labeling of hippocampal astrocytes
title_full_unstemmed The multispecific thyroid hormone transporter OATP1C1 mediates cell-specific sulforhodamine 101-labeling of hippocampal astrocytes
title_short The multispecific thyroid hormone transporter OATP1C1 mediates cell-specific sulforhodamine 101-labeling of hippocampal astrocytes
title_sort multispecific thyroid hormone transporter oatp1c1 mediates cell-specific sulforhodamine 101-labeling of hippocampal astrocytes
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4286632/
https://www.ncbi.nlm.nih.gov/pubmed/24129767
http://dx.doi.org/10.1007/s00429-013-0645-0
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