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Expression of Ezrin in Vagina Cells of Postmenopausal Rats after Dietary Administration of Omega-3 Fatty Acid Formula

OBJECTIVES: To see the effect of dietary administration of omega 3-fatty acid formula on the vaginal cells of postmenopausal rats. METHODS: Three-week-old female Wistar/ST rats were raised after one week of adjustment period. The rats were then divided into three groups, for three different kinds of...

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Autores principales: Lee, Hae-Hyeog, Kim, Tae-Hee, Park, Junsik, Lee, Arum, Park, Yongsoon, Byun, Dong Won, Kim, Min Jung, Lim, Heesook
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Korean Society of Menopause 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4286661/
https://www.ncbi.nlm.nih.gov/pubmed/25580420
http://dx.doi.org/10.6118/jmm.2014.20.3.97
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author Lee, Hae-Hyeog
Kim, Tae-Hee
Park, Junsik
Lee, Arum
Park, Yongsoon
Byun, Dong Won
Kim, Min Jung
Lim, Heesook
author_facet Lee, Hae-Hyeog
Kim, Tae-Hee
Park, Junsik
Lee, Arum
Park, Yongsoon
Byun, Dong Won
Kim, Min Jung
Lim, Heesook
author_sort Lee, Hae-Hyeog
collection PubMed
description OBJECTIVES: To see the effect of dietary administration of omega 3-fatty acid formula on the vaginal cells of postmenopausal rats. METHODS: Three-week-old female Wistar/ST rats were raised after one week of adjustment period. The rats were then divided into three groups, for three different kinds of diet; general diet, 1% omega-3 fatty acid diet, and 2% omega-3 fatty acid diet. After eight weeks of having assigned diet, after the oophorectomy, with the same diet previously they had Immunohistochemistry, Immunofluorescence, and Western Blot about ezrin, merlin were done. RESULTS: In immunohistochemistry, estrogen injection group revealed thicker and well differentiated features. In Immunofluorescence, Omega-3 fatty acid composition in diet did not effect expression of ezrin and merlin in rat vagina in estrogen injection group, their vaginal epithelium showed full layers (from basal to apical layer). In Western Blot analysis, Omega-3 fatty acid composition in diet did not affect expression of ezrin and merlin in rat vagina estrogen presented significant impact on expression of ezrin and merlin. CONCLUSION: Although omega-3 fatty acid composition changed in diet, vaginal epithelial morphology unchanged. Estrogen did effect on vagina cell, but omega-3 fatty acid did not effect on ezrin and merlin in vagina.
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spelling pubmed-42866612015-01-11 Expression of Ezrin in Vagina Cells of Postmenopausal Rats after Dietary Administration of Omega-3 Fatty Acid Formula Lee, Hae-Hyeog Kim, Tae-Hee Park, Junsik Lee, Arum Park, Yongsoon Byun, Dong Won Kim, Min Jung Lim, Heesook J Menopausal Med Original Article OBJECTIVES: To see the effect of dietary administration of omega 3-fatty acid formula on the vaginal cells of postmenopausal rats. METHODS: Three-week-old female Wistar/ST rats were raised after one week of adjustment period. The rats were then divided into three groups, for three different kinds of diet; general diet, 1% omega-3 fatty acid diet, and 2% omega-3 fatty acid diet. After eight weeks of having assigned diet, after the oophorectomy, with the same diet previously they had Immunohistochemistry, Immunofluorescence, and Western Blot about ezrin, merlin were done. RESULTS: In immunohistochemistry, estrogen injection group revealed thicker and well differentiated features. In Immunofluorescence, Omega-3 fatty acid composition in diet did not effect expression of ezrin and merlin in rat vagina in estrogen injection group, their vaginal epithelium showed full layers (from basal to apical layer). In Western Blot analysis, Omega-3 fatty acid composition in diet did not affect expression of ezrin and merlin in rat vagina estrogen presented significant impact on expression of ezrin and merlin. CONCLUSION: Although omega-3 fatty acid composition changed in diet, vaginal epithelial morphology unchanged. Estrogen did effect on vagina cell, but omega-3 fatty acid did not effect on ezrin and merlin in vagina. The Korean Society of Menopause 2014-12 2014-12-24 /pmc/articles/PMC4286661/ /pubmed/25580420 http://dx.doi.org/10.6118/jmm.2014.20.3.97 Text en Copyright © 2014 by The Korean Society of Menopause http://creativecommons.org/licenses/by-nc/3.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/).
spellingShingle Original Article
Lee, Hae-Hyeog
Kim, Tae-Hee
Park, Junsik
Lee, Arum
Park, Yongsoon
Byun, Dong Won
Kim, Min Jung
Lim, Heesook
Expression of Ezrin in Vagina Cells of Postmenopausal Rats after Dietary Administration of Omega-3 Fatty Acid Formula
title Expression of Ezrin in Vagina Cells of Postmenopausal Rats after Dietary Administration of Omega-3 Fatty Acid Formula
title_full Expression of Ezrin in Vagina Cells of Postmenopausal Rats after Dietary Administration of Omega-3 Fatty Acid Formula
title_fullStr Expression of Ezrin in Vagina Cells of Postmenopausal Rats after Dietary Administration of Omega-3 Fatty Acid Formula
title_full_unstemmed Expression of Ezrin in Vagina Cells of Postmenopausal Rats after Dietary Administration of Omega-3 Fatty Acid Formula
title_short Expression of Ezrin in Vagina Cells of Postmenopausal Rats after Dietary Administration of Omega-3 Fatty Acid Formula
title_sort expression of ezrin in vagina cells of postmenopausal rats after dietary administration of omega-3 fatty acid formula
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4286661/
https://www.ncbi.nlm.nih.gov/pubmed/25580420
http://dx.doi.org/10.6118/jmm.2014.20.3.97
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