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Evaluation of the efficacy of 2% Ocimum sanctum gel in the treatment of experimental periodontitis
INTRODUCTION: One of the options for the treatment of periodontitis is local drug delivery systems (LDD). Tulsi (Ocimum sanctum), a traditional herb, has many uses in medicine. It could be a suitable agent as LDD for the treatment of periodontitis. AIM: The aim was to formulate, evaluate the anti-in...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Medknow Publications & Media Pvt Ltd
2015
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4286833/ https://www.ncbi.nlm.nih.gov/pubmed/25599031 http://dx.doi.org/10.4103/2230-973X.147231 |
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author | Hosadurga, Rajesh Ramesh Rao, Sudarshan Narayan Edavanputhalath, Rejeesh Jose, Jobin Rompicharla, Narayana Charyulu Shakil, Moidin Raju, Shashidhara |
author_facet | Hosadurga, Rajesh Ramesh Rao, Sudarshan Narayan Edavanputhalath, Rejeesh Jose, Jobin Rompicharla, Narayana Charyulu Shakil, Moidin Raju, Shashidhara |
author_sort | Hosadurga, Rajesh Ramesh |
collection | PubMed |
description | INTRODUCTION: One of the options for the treatment of periodontitis is local drug delivery systems (LDD). Tulsi (Ocimum sanctum), a traditional herb, has many uses in medicine. It could be a suitable agent as LDD for the treatment of periodontitis. AIM: The aim was to formulate, evaluate the anti-inflammatory activity; assess duration of the action and the efficacy of 2% tulsi (O. sanctum) gel in the treatment of experimental periodontitis in Wistar Albino rat model. SETTINGS AND DESIGN: Thirty six Wistar albino rats were randomly assigned to 3 groups. Periodontitis was induced using ligature model. Group 1-control; Group 2-Plain gel and Group 3-2% tulsi (O. sanctum) gel. MATERIALS AND METHODS: 2% tulsi (O. sanctum) gel were prepared. The anti-inflammatory activity and duration of action were assessed. Silk ligature 5-0 was used to induce periodontitis. Gingival index (GI) and probing pocket depth were measured. Treatment was done. The rats were sacrificed. Morphometric analysis was done using Stereomicroscope and ImageJ software. STATISTICAL ANALYSIS USED: ANOVA followed by Bonferroni's test, Wilcoxon's test for intergroup comparison, Mann-Whitney test for P value computation was used. The observations are mean ± standard deviation and standard error of the mean. P < 0.01 as compared to control was considered as statistically significant. RESULTS: 2% tulsi (O. sanctum) gel showed 33.66% inhibition of edema and peak activity was noted at 24 h. There was statistically significant change in the GI and probing pocket depth. Morphometric analysis did not show any significant difference between groups. No toxic effects were seen on oral administration of 2000 mg/kg of Tulsi extract. CONCLUSIONS: 2% tulsi (O. sanctum) gel was effective in the treatment of experimental periodontitis. |
format | Online Article Text |
id | pubmed-4286833 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Medknow Publications & Media Pvt Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-42868332015-01-16 Evaluation of the efficacy of 2% Ocimum sanctum gel in the treatment of experimental periodontitis Hosadurga, Rajesh Ramesh Rao, Sudarshan Narayan Edavanputhalath, Rejeesh Jose, Jobin Rompicharla, Narayana Charyulu Shakil, Moidin Raju, Shashidhara Int J Pharm Investig Original Research Article INTRODUCTION: One of the options for the treatment of periodontitis is local drug delivery systems (LDD). Tulsi (Ocimum sanctum), a traditional herb, has many uses in medicine. It could be a suitable agent as LDD for the treatment of periodontitis. AIM: The aim was to formulate, evaluate the anti-inflammatory activity; assess duration of the action and the efficacy of 2% tulsi (O. sanctum) gel in the treatment of experimental periodontitis in Wistar Albino rat model. SETTINGS AND DESIGN: Thirty six Wistar albino rats were randomly assigned to 3 groups. Periodontitis was induced using ligature model. Group 1-control; Group 2-Plain gel and Group 3-2% tulsi (O. sanctum) gel. MATERIALS AND METHODS: 2% tulsi (O. sanctum) gel were prepared. The anti-inflammatory activity and duration of action were assessed. Silk ligature 5-0 was used to induce periodontitis. Gingival index (GI) and probing pocket depth were measured. Treatment was done. The rats were sacrificed. Morphometric analysis was done using Stereomicroscope and ImageJ software. STATISTICAL ANALYSIS USED: ANOVA followed by Bonferroni's test, Wilcoxon's test for intergroup comparison, Mann-Whitney test for P value computation was used. The observations are mean ± standard deviation and standard error of the mean. P < 0.01 as compared to control was considered as statistically significant. RESULTS: 2% tulsi (O. sanctum) gel showed 33.66% inhibition of edema and peak activity was noted at 24 h. There was statistically significant change in the GI and probing pocket depth. Morphometric analysis did not show any significant difference between groups. No toxic effects were seen on oral administration of 2000 mg/kg of Tulsi extract. CONCLUSIONS: 2% tulsi (O. sanctum) gel was effective in the treatment of experimental periodontitis. Medknow Publications & Media Pvt Ltd 2015 /pmc/articles/PMC4286833/ /pubmed/25599031 http://dx.doi.org/10.4103/2230-973X.147231 Text en Copyright: © International Journal of Pharmaceutical Investigation http://creativecommons.org/licenses/by-nc-sa/3.0 This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Research Article Hosadurga, Rajesh Ramesh Rao, Sudarshan Narayan Edavanputhalath, Rejeesh Jose, Jobin Rompicharla, Narayana Charyulu Shakil, Moidin Raju, Shashidhara Evaluation of the efficacy of 2% Ocimum sanctum gel in the treatment of experimental periodontitis |
title | Evaluation of the efficacy of 2% Ocimum sanctum gel in the treatment of experimental periodontitis |
title_full | Evaluation of the efficacy of 2% Ocimum sanctum gel in the treatment of experimental periodontitis |
title_fullStr | Evaluation of the efficacy of 2% Ocimum sanctum gel in the treatment of experimental periodontitis |
title_full_unstemmed | Evaluation of the efficacy of 2% Ocimum sanctum gel in the treatment of experimental periodontitis |
title_short | Evaluation of the efficacy of 2% Ocimum sanctum gel in the treatment of experimental periodontitis |
title_sort | evaluation of the efficacy of 2% ocimum sanctum gel in the treatment of experimental periodontitis |
topic | Original Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4286833/ https://www.ncbi.nlm.nih.gov/pubmed/25599031 http://dx.doi.org/10.4103/2230-973X.147231 |
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