Histology-Directed Microwave Assisted Enzymatic Protein Digestion for MALDI MS Analysis of Mammalian Tissue

[Image: see text] This study presents on-tissue proteolytic digestion using a microwave irradiation and peptide extraction method for in situ analysis of proteins from spatially defined regions of a tissue section. The methodology utilizes hydrogel discs (1 mm diameter) embedded with trypsin solutio...

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Detalles Bibliográficos
Autores principales: Taverna, Domenico, Norris, Jeremy L., Caprioli, Richard M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2014
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4287167/
https://www.ncbi.nlm.nih.gov/pubmed/25427280
http://dx.doi.org/10.1021/ac503479a

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4287167/
https://www.ncbi.nlm.nih.gov/pubmed/25427280
http://dx.doi.org/10.1021/ac503479a

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