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Histology-Directed Microwave Assisted Enzymatic Protein Digestion for MALDI MS Analysis of Mammalian Tissue

[Image: see text] This study presents on-tissue proteolytic digestion using a microwave irradiation and peptide extraction method for in situ analysis of proteins from spatially defined regions of a tissue section. The methodology utilizes hydrogel discs (1 mm diameter) embedded with trypsin solutio...

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Detalles Bibliográficos
Autores principales:	Taverna, Domenico, Norris, Jeremy L., Caprioli, Richard M.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	American Chemical Society 2014
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4287167/ https://www.ncbi.nlm.nih.gov/pubmed/25427280 http://dx.doi.org/10.1021/ac503479a

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