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Analysis and Distribution of Esculetin in Plasma and Tissues of Rats after Oral Administration

In this study, we developed a method to quantify esculetin (6,7-dihydroxycoumarin) in plasma and tissues using HPLC coupled with ultraviolet detection and measured the level of esculetin in rat plasma after oral administration. The calibration curve for esculetin was linear in the range of 4.8 ng/mL...

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Autores principales: Kim, Ji-Sun, Ha, Tae-Youl, Ahn, Jiyun, Kim, Suna
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Korean Society of Food Science and Nutrition 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4287325/
https://www.ncbi.nlm.nih.gov/pubmed/25580397
http://dx.doi.org/10.3746/pnf.2014.19.4.321
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author Kim, Ji-Sun
Ha, Tae-Youl
Ahn, Jiyun
Kim, Suna
author_facet Kim, Ji-Sun
Ha, Tae-Youl
Ahn, Jiyun
Kim, Suna
author_sort Kim, Ji-Sun
collection PubMed
description In this study, we developed a method to quantify esculetin (6,7-dihydroxycoumarin) in plasma and tissues using HPLC coupled with ultraviolet detection and measured the level of esculetin in rat plasma after oral administration. The calibration curve for esculetin was linear in the range of 4.8 ng/mL to 476.2 ng/mL, with a correlation coefficient (r(2)) of 0.996, a limit of detection value of 33.2 ng/mL, and a limit of quantification value of 100.6 ng/mL. Recovery rates for the 95.2 ng/mL and 190.5 ng/mL samples were 95.2% and 100.3%, within-runs and 104.8% and 101.0% between-runs, respectively. The relative standard deviation was less than 7% for both runs. In the pharmacokinetic analysis, the peak plasma esculetin level was reached 5 min after administration (C(max)=173.3 ng/mL; T(1/2)=45 min; AUC(0 ~180 min)=5,167.5 ng · min/mL). At 180 min post-administration (i.e., after euthanasia), esculetin was only detectable in the liver (30.87±11.33 ng/g) and the kidney (20.29±7.02 ng/g).
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spelling pubmed-42873252015-01-09 Analysis and Distribution of Esculetin in Plasma and Tissues of Rats after Oral Administration Kim, Ji-Sun Ha, Tae-Youl Ahn, Jiyun Kim, Suna Prev Nutr Food Sci Articles In this study, we developed a method to quantify esculetin (6,7-dihydroxycoumarin) in plasma and tissues using HPLC coupled with ultraviolet detection and measured the level of esculetin in rat plasma after oral administration. The calibration curve for esculetin was linear in the range of 4.8 ng/mL to 476.2 ng/mL, with a correlation coefficient (r(2)) of 0.996, a limit of detection value of 33.2 ng/mL, and a limit of quantification value of 100.6 ng/mL. Recovery rates for the 95.2 ng/mL and 190.5 ng/mL samples were 95.2% and 100.3%, within-runs and 104.8% and 101.0% between-runs, respectively. The relative standard deviation was less than 7% for both runs. In the pharmacokinetic analysis, the peak plasma esculetin level was reached 5 min after administration (C(max)=173.3 ng/mL; T(1/2)=45 min; AUC(0 ~180 min)=5,167.5 ng · min/mL). At 180 min post-administration (i.e., after euthanasia), esculetin was only detectable in the liver (30.87±11.33 ng/g) and the kidney (20.29±7.02 ng/g). The Korean Society of Food Science and Nutrition 2014-12 2014-12-31 /pmc/articles/PMC4287325/ /pubmed/25580397 http://dx.doi.org/10.3746/pnf.2014.19.4.321 Text en Copyright © 2014 by The Korean Society of Food Science and Nutrition. All rights Reserved. This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Articles
Kim, Ji-Sun
Ha, Tae-Youl
Ahn, Jiyun
Kim, Suna
Analysis and Distribution of Esculetin in Plasma and Tissues of Rats after Oral Administration
title Analysis and Distribution of Esculetin in Plasma and Tissues of Rats after Oral Administration
title_full Analysis and Distribution of Esculetin in Plasma and Tissues of Rats after Oral Administration
title_fullStr Analysis and Distribution of Esculetin in Plasma and Tissues of Rats after Oral Administration
title_full_unstemmed Analysis and Distribution of Esculetin in Plasma and Tissues of Rats after Oral Administration
title_short Analysis and Distribution of Esculetin in Plasma and Tissues of Rats after Oral Administration
title_sort analysis and distribution of esculetin in plasma and tissues of rats after oral administration
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4287325/
https://www.ncbi.nlm.nih.gov/pubmed/25580397
http://dx.doi.org/10.3746/pnf.2014.19.4.321
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