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The genome of Eimeria falciformis - reduction and specialization in a single host apicomplexan parasite

BACKGROUND: The phylum Apicomplexa comprises important unicellular human parasites such as Toxoplasma and Plasmodium. Eimeria is the largest and most diverse genus of apicomplexan parasites and some species of the genus are the causative agent of coccidiosis, a disease economically devastating in po...

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Autores principales: Heitlinger, Emanuel, Spork, Simone, Lucius, Richard, Dieterich, Christoph
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4287421/
https://www.ncbi.nlm.nih.gov/pubmed/25142335
http://dx.doi.org/10.1186/1471-2164-15-696
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author Heitlinger, Emanuel
Spork, Simone
Lucius, Richard
Dieterich, Christoph
author_facet Heitlinger, Emanuel
Spork, Simone
Lucius, Richard
Dieterich, Christoph
author_sort Heitlinger, Emanuel
collection PubMed
description BACKGROUND: The phylum Apicomplexa comprises important unicellular human parasites such as Toxoplasma and Plasmodium. Eimeria is the largest and most diverse genus of apicomplexan parasites and some species of the genus are the causative agent of coccidiosis, a disease economically devastating in poultry. We report a complete genome sequence of the mouse parasite Eimeria falciformis. We assembled and annotated the genome sequence to study host-parasite interactions in this understudied genus in a model organism host. RESULTS: The genome of E. falciformis is 44 Mb in size and contains 5,879 predicted protein coding genes. Comparative analysis of E. falciformis with Toxoplasma gondii shows an emergence and diversification of gene families associated with motility and invasion mainly at the level of the Coccidia. Many rhoptry kinases, among them important virulence factors in T. gondii, are absent from the E. falciformis genome. Surface antigens are divergent between Eimeria species. Comparisons with T. gondii showed differences between genes involved in metabolism, N-glycan and GPI-anchor synthesis. E. falciformis possesses a reduced set of transmembrane transporters and we suggest an altered mode of iron uptake in the genus Eimeria. CONCLUSIONS: Reduced diversity of genes required for host-parasite interaction and transmembrane transport allow hypotheses on host adaptation and specialization of a single host parasite. The E. falciformis genome sequence sheds light on the evolution of the Coccidia and helps to identify determinants of host-parasite interaction critical for drug and vaccine development. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/1471-2164-15-696) contains supplementary material, which is available to authorized users.
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spelling pubmed-42874212015-01-09 The genome of Eimeria falciformis - reduction and specialization in a single host apicomplexan parasite Heitlinger, Emanuel Spork, Simone Lucius, Richard Dieterich, Christoph BMC Genomics Research Article BACKGROUND: The phylum Apicomplexa comprises important unicellular human parasites such as Toxoplasma and Plasmodium. Eimeria is the largest and most diverse genus of apicomplexan parasites and some species of the genus are the causative agent of coccidiosis, a disease economically devastating in poultry. We report a complete genome sequence of the mouse parasite Eimeria falciformis. We assembled and annotated the genome sequence to study host-parasite interactions in this understudied genus in a model organism host. RESULTS: The genome of E. falciformis is 44 Mb in size and contains 5,879 predicted protein coding genes. Comparative analysis of E. falciformis with Toxoplasma gondii shows an emergence and diversification of gene families associated with motility and invasion mainly at the level of the Coccidia. Many rhoptry kinases, among them important virulence factors in T. gondii, are absent from the E. falciformis genome. Surface antigens are divergent between Eimeria species. Comparisons with T. gondii showed differences between genes involved in metabolism, N-glycan and GPI-anchor synthesis. E. falciformis possesses a reduced set of transmembrane transporters and we suggest an altered mode of iron uptake in the genus Eimeria. CONCLUSIONS: Reduced diversity of genes required for host-parasite interaction and transmembrane transport allow hypotheses on host adaptation and specialization of a single host parasite. The E. falciformis genome sequence sheds light on the evolution of the Coccidia and helps to identify determinants of host-parasite interaction critical for drug and vaccine development. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/1471-2164-15-696) contains supplementary material, which is available to authorized users. BioMed Central 2014-08-20 /pmc/articles/PMC4287421/ /pubmed/25142335 http://dx.doi.org/10.1186/1471-2164-15-696 Text en © Heitlinger et al.; licensee BioMed Central Ltd. 2014 This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research Article
Heitlinger, Emanuel
Spork, Simone
Lucius, Richard
Dieterich, Christoph
The genome of Eimeria falciformis - reduction and specialization in a single host apicomplexan parasite
title The genome of Eimeria falciformis - reduction and specialization in a single host apicomplexan parasite
title_full The genome of Eimeria falciformis - reduction and specialization in a single host apicomplexan parasite
title_fullStr The genome of Eimeria falciformis - reduction and specialization in a single host apicomplexan parasite
title_full_unstemmed The genome of Eimeria falciformis - reduction and specialization in a single host apicomplexan parasite
title_short The genome of Eimeria falciformis - reduction and specialization in a single host apicomplexan parasite
title_sort genome of eimeria falciformis - reduction and specialization in a single host apicomplexan parasite
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4287421/
https://www.ncbi.nlm.nih.gov/pubmed/25142335
http://dx.doi.org/10.1186/1471-2164-15-696
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