Cargando…

KRAS Mutation Analysis by PCR: A Comparison of Two Methods

BACKGROUND: KRAS mutation assays are important companion diagnostic tests to guide anti-EGFR antibody treatment of metastatic colorectal cancer. Direct comparison of newer diagnostic methods with existing methods is an important part of validation of any new technique. In this this study, we have co...

Descripción completa

Detalles Bibliográficos
Autores principales:	Bolton, Louise, Reiman, Anne, Lucas, Katie, Timms, Judith, Cree, Ian A.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Public Library of Science 2015
Materias:	Research Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4287618/ https://www.ncbi.nlm.nih.gov/pubmed/25568935 http://dx.doi.org/10.1371/journal.pone.0115672

Ejemplares similares

Diagnostic RAS mutation analysis by polymerase chain reaction (PCR)
por: Cree, Ian A.
Publicado: (2016)

Improvement of digital PCR conditions for direct detection of KRAS mutations
por: Lee, Jina, et al.
Publicado: (2020)

Validation of an NGS mutation detection panel for melanoma
por: Reiman, Anne, et al.
Publicado: (2017)

COLD-PCR enhanced melting curve analysis improves diagnostic accuracy for KRAS mutations in colorectal carcinoma
por: Pritchard, Colin C, et al.
Publicado: (2010)

High sensitivity of both sequencing and real-time PCR analysis of KRAS mutations in colorectal cancer tissue
por: Tol, Jolien, et al.
Publicado: (2010)

Optimized Multiplex Detection of 7 KRAS Mutations by Taqman Allele-Specific qPCR
por: Orue, Andrea, et al.
Publicado: (2016)

Allele-Specific PCR for KRAS Mutation Detection Using Phosphoryl Guanidine Modified Primers
por: Chubarov, Alexey S., et al.
Publicado: (2020)

KRAS genotyping by digital PCR combined with melting curve analysis
por: Tanaka, Junko, et al.
Publicado: (2019)

A comparison of three methods for detecting KRAS mutations in formalin-fixed colorectal cancer specimens
por: Gonzalez de Castro, D, et al.
Publicado: (2012)

A Chamber-Based Digital PCR Based on a Microfluidic Chip for the Absolute Quantification and Analysis of KRAS Mutation
por: Ren, Jie, et al.
Publicado: (2023)

CRE: a cost effective and rapid approach for PCR-mediated concatenation of KRAS and EGFR exons: Rapid way to detect EGFR and KRAS mutations
por: Ramteke, Manoj P., et al.
Publicado: (2016)

SensiScreen(®) KRAS exon 2-sensitive simplex and multiplex real-time PCR-based assays for detection of KRAS exon 2 mutations
por: Riva, Alice, et al.
Publicado: (2017)

Measuring KRAS Mutations in Circulating Tumor DNA by Droplet Digital PCR and Next-Generation Sequencing
por: Demuth, Christina, et al.
Publicado: (2018)

A Cross-Sectional Study for Evaluation of KRAS and BRAF Mutations by Reverse Dot Blot, PCR-RFLP, and Allele-Specific PCR Methods Among Patients with Colorectal Cancer
por: Sheikhsofla, Fatemeh, et al.
Publicado: (2021)

Snapback Primer Mediated Clamping PCR for Detection of EGFR and KRAS Mutations in NSCLC Patients by High Resolution Melting Analysis
por: Sun, Haiyan, et al.
Publicado: (2014)

Comment on ‘A comparison of three methods for detecting KRAS mutations in formalin-fixed colorectal cancer specimens’
por: Normanno, N, et al.
Publicado: (2012)

Detecting KRAS mutations in peripheral blood of colorectal cancer patients by peptide nucleic acid clamp PCR
por: MIYANO, SHOZO, et al.
Publicado: (2012)

Multi-Center Evaluation of the Fully Automated PCR-Based Idylla(™) KRAS Mutation Assay for Rapid KRAS Mutation Status Determination on Formalin-Fixed Paraffin-Embedded Tissue of Human Colorectal Cancer
por: Solassol, Jérôme, et al.
Publicado: (2016)

FLAG assay as a novel method for real-time signal generation during PCR: application to detection and genotyping of KRAS codon 12 mutations
por: Amicarelli, Giulia, et al.
Publicado: (2007)

Reliability of digital PCR in detecting KRAS mutation in colorectal cancer using plasma sample: A systematic review and meta-analysis
por: Ye, Peng, et al.
Publicado: (2020)

Allele Specific Locked Nucleic Acid Quantitative PCR (ASLNAqPCR): An Accurate and Cost-Effective Assay to Diagnose and Quantify KRAS and BRAF Mutation
por: Morandi, Luca, et al.
Publicado: (2012)

Dysregulation of KRAS signaling in pancreatic cancer is not associated with KRAS mutations and outcome
por: Lemstrova, Radmila, et al.
Publicado: (2017)

KRAS Mutation Test in Korean Patients with Colorectal Carcinomas: A Methodological Comparison between Sanger Sequencing and a Real-Time PCR-Based Assay
por: Lee, Sung Hak, et al.
Publicado: (2017)

Evaluation of droplet digital PCR and next generation sequencing for characterizing DNA reference material for KRAS mutation detection
por: Dong, Lianhua, et al.
Publicado: (2018)

Wild-type blocking pcr coupled with internal competitive amplified fragment improved the detection of rare mutation of KRAS
por: Peng, Jia, et al.
Publicado: (2017)

KRAS mutational analysis for colorectal cancer: Application: Pharmacogenomic
por: Wang, Grace, et al.
Publicado: (2010)

Application of PCR methods to evaluate EGFR, KRAS and BRAF mutations in a small number of tumor cells in cytological material from lung cancer patients
por: LEWANDOWSKA, MARZENA ANNA, et al.
Publicado: (2013)

Computational Analysis of KRAS Mutations: Implications for Different Effects on the KRAS p.G12D and p.G13D Mutations
por: Chen, Chih-Chieh, et al.
Publicado: (2013)

Liquid biopsy in colon cancer: comparison of different circulating DNA extraction systems following absolute quantification of KRAS mutations using Intplex allele-specific PCR
por: Kloten, Vera, et al.
Publicado: (2017)

KRAS mutations and endometriosis burden of disease
por: Orr, Natasha L, et al.
Publicado: (2023)

Use of the QIAGEN GeneReader NGS system for detection of KRAS mutations, validated by the QIAGEN Therascreen PCR kit and alternative NGS platform
por: Darwanto, Agus, et al.
Publicado: (2017)

Optimization of routine KRAS mutation PCR-based testing procedure for rational individualized first-line-targeted therapy selection in metastatic colorectal cancer
por: Chretien, Anne-Sophie, et al.
Publicado: (2013)

KRAS analysis in colorectal carcinoma: Analytical aspects of Pyrosequencing and allele-specific PCR in clinical practice
por: Sundström, Magnus, et al.
Publicado: (2010)

Analysis of KRAS Mutations of Exon 2 Codons 12 and 13 by SNaPshot Analysis in Comparison to Common DNA Sequencing
por: Zinsky, Rica, et al.
Publicado: (2010)

Single-Tubed Wild-Type Blocking Quantitative PCR Detection Assay for the Sensitive Detection of Codon 12 and 13 KRAS Mutations
por: Huang, Jun-Fu, et al.
Publicado: (2015)

KRAS G12V Mutation Detection by Droplet Digital PCR in Circulating Cell-Free DNA of Colorectal Cancer Patients
por: Olmedillas López, Susana, et al.
Publicado: (2016)

Accurate detection of KRAS, NRAS and BRAF mutations in metastatic colorectal cancers by bridged nucleic acid-clamp real-time PCR
por: Nagakubo, Yuki, et al.
Publicado: (2019)

Frequent mutations of KRAS in addition to BRAF in colorectal serrated adenocarcinoma
por: Stefanius, Karoliina, et al.
Publicado: (2011)

Spatial heterogeneity of KRAS mutations in colorectal cancers in northern France
por: Turpin, Anthony, et al.
Publicado: (2019)

Characteristics and prognostic analysis of patients with detected KRAS mutations in resected lung adenocarcinomas by peptide nucleic acid-locked nucleic acid polymerase chain reaction (PNA-LNA PCR) clamp method
por: Hashimoto, Tatsuya, et al.
Publicado: (2023)

Cannot write session to /tmp/vufind_sessions/sess_r5pd10pcsq1dckcp79q846apk3