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Biosensor Applications of MAPLE Deposited Lipase

Matrix Assisted Pulsed Laser Evaporation (MAPLE) is a thin film deposition technique derived from Pulsed Laser Deposition (PLD) for deposition of delicate (polymers, complex biological molecules, etc.) materials in undamaged form. The main difference of MAPLE technique with respect to PLD is the tar...

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Autores principales: Califano, Valeria, Bloisi, Francesco, Aronne, Antonio, Federici, Stefania, Nasti, Libera, Depero, Laura E., Vicari, Luciano R. M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4287705/
https://www.ncbi.nlm.nih.gov/pubmed/25587426
http://dx.doi.org/10.3390/bios4040329
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author Califano, Valeria
Bloisi, Francesco
Aronne, Antonio
Federici, Stefania
Nasti, Libera
Depero, Laura E.
Vicari, Luciano R. M.
author_facet Califano, Valeria
Bloisi, Francesco
Aronne, Antonio
Federici, Stefania
Nasti, Libera
Depero, Laura E.
Vicari, Luciano R. M.
author_sort Califano, Valeria
collection PubMed
description Matrix Assisted Pulsed Laser Evaporation (MAPLE) is a thin film deposition technique derived from Pulsed Laser Deposition (PLD) for deposition of delicate (polymers, complex biological molecules, etc.) materials in undamaged form. The main difference of MAPLE technique with respect to PLD is the target: it is a frozen solution or suspension of the (guest) molecules to be deposited in a volatile substance (matrix). Since laser beam energy is mainly absorbed by the matrix, damages to the delicate guest molecules are avoided, or at least reduced. Lipase, an enzyme catalyzing reactions borne by triglycerides, has been used in biosensors for detection of β-hydroxyacid esters and triglycerides in blood serum. Enzymes immobilization on a substrate is therefore required. In this paper we show that it is possible, using MAPLE technique, to deposit lipase on a substrate, as shown by AFM observation, preserving its conformational structure, as shown by FTIR analysis.
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spelling pubmed-42877052015-01-13 Biosensor Applications of MAPLE Deposited Lipase Califano, Valeria Bloisi, Francesco Aronne, Antonio Federici, Stefania Nasti, Libera Depero, Laura E. Vicari, Luciano R. M. Biosensors (Basel) Communication Matrix Assisted Pulsed Laser Evaporation (MAPLE) is a thin film deposition technique derived from Pulsed Laser Deposition (PLD) for deposition of delicate (polymers, complex biological molecules, etc.) materials in undamaged form. The main difference of MAPLE technique with respect to PLD is the target: it is a frozen solution or suspension of the (guest) molecules to be deposited in a volatile substance (matrix). Since laser beam energy is mainly absorbed by the matrix, damages to the delicate guest molecules are avoided, or at least reduced. Lipase, an enzyme catalyzing reactions borne by triglycerides, has been used in biosensors for detection of β-hydroxyacid esters and triglycerides in blood serum. Enzymes immobilization on a substrate is therefore required. In this paper we show that it is possible, using MAPLE technique, to deposit lipase on a substrate, as shown by AFM observation, preserving its conformational structure, as shown by FTIR analysis. MDPI 2014-10-03 /pmc/articles/PMC4287705/ /pubmed/25587426 http://dx.doi.org/10.3390/bios4040329 Text en © 2014 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Communication
Califano, Valeria
Bloisi, Francesco
Aronne, Antonio
Federici, Stefania
Nasti, Libera
Depero, Laura E.
Vicari, Luciano R. M.
Biosensor Applications of MAPLE Deposited Lipase
title Biosensor Applications of MAPLE Deposited Lipase
title_full Biosensor Applications of MAPLE Deposited Lipase
title_fullStr Biosensor Applications of MAPLE Deposited Lipase
title_full_unstemmed Biosensor Applications of MAPLE Deposited Lipase
title_short Biosensor Applications of MAPLE Deposited Lipase
title_sort biosensor applications of maple deposited lipase
topic Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4287705/
https://www.ncbi.nlm.nih.gov/pubmed/25587426
http://dx.doi.org/10.3390/bios4040329
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