Association of the histone-lysine N-methyltransferase MLL5 gene with coronary artery disease in Chinese Han people

BACKGROUND: MLL5, a member of the histone-lysine N-methyltransferase family, has been implicated in the control of the cell cycle progression and survival. The aim of this study was to explore the relationship between the interaction of histone-lysine N-methyltransferase MLL5 gene polymorphism and CAD in a Chinese Han population. METHODS: Using a case–control study of Chinese CAD patients (n = 565) and healthy controls (n = 694), we investigated the MLL5 gene polymorphism by the use of polymerase chain reaction fragment length polymorphism (PCR–RFLP) analysis. RESULTS: For total, the distribution of SNP1 (rs12671368) and SNP2 (rs2192932) genotypes showed a significant difference between CAD and control participants (P(1) = 0.03, P(2) = 0.02). For total the distribution of SNP1 (rs12671368) and SNP2 (rs2192932) alleles in the dominant model (GG vs. AA + AG) and the recessive model (AA vs. AG + GG) showed a significant difference between CAD and control participants (for allele: P(1) < 0.01 and P(2) = 0.05, for dominant model: P(1) > 0.05 and P(2) = 0.02, for recessive model: P(1) = 0.03 and P(2) = 0.78, respectively). For total the significant difference of the distribution of SNP1 and SNP2 in the dominant model and recessive model was retained after adjusting for covariates (for dominant model: SNP1 OR: 1.68, 95% confidence interval [CI]: 1.08–2.64, P = 0.02; SNP2 OR: 0.51, 95% CI: 0.36–0.72, P = 0.01; for recessive model: SNP1 OR: 1.84, 95% confidence interval [CI]: 1.28–2.64, P < 0.01; SNP2 OR: 0.65, 95% CI: 0.35–1.22, P = 0.18). CONCLUSIONS: The GG genotype of rs12671368 and the AA genotype of rs2192932 in the MLL5 gene could be protective genetic markers of CAD.