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Genetic diversity and relationship of Hedychium from Northeast India as dissected using PCA analysis and hierarchical clustering
Molecular genetic fingerprints of eleven Hedychium species from Northeast India were developed using PCR based markers. Fifteen inter-simple sequence repeats (ISSRs) and five amplified fragment length polymorphism (AFLP) primers produced 547 polymorphic fragments. Positive correlation (r = 0.46) was...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4287868/ https://www.ncbi.nlm.nih.gov/pubmed/25606430 http://dx.doi.org/10.1016/j.mgene.2014.05.002 |
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author | Basak, Supriyo Ramesh, Aadi Moolam Kesari, Vigya Parida, Ajay Mitra, Sudip Rangan, Latha |
author_facet | Basak, Supriyo Ramesh, Aadi Moolam Kesari, Vigya Parida, Ajay Mitra, Sudip Rangan, Latha |
author_sort | Basak, Supriyo |
collection | PubMed |
description | Molecular genetic fingerprints of eleven Hedychium species from Northeast India were developed using PCR based markers. Fifteen inter-simple sequence repeats (ISSRs) and five amplified fragment length polymorphism (AFLP) primers produced 547 polymorphic fragments. Positive correlation (r = 0.46) was observed between the mean genetic similarity and genetic diversity parameters at the inter-species level. AFLP and ISSR markers were able to group the species according to its altitude and intensity of flower aroma. Cophenetic correlation coefficients between the dendrogram and the original similarity matrix were significant for ISSR (r = 0.89) compared to AFLP (r = 0.83) markers. This genetic characterization of Hedychium from Northeast India contributes to the knowledge of genetic structure of the species and can be used to define strategies for their conservation and management. |
format | Online Article Text |
id | pubmed-4287868 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-42878682015-01-20 Genetic diversity and relationship of Hedychium from Northeast India as dissected using PCA analysis and hierarchical clustering Basak, Supriyo Ramesh, Aadi Moolam Kesari, Vigya Parida, Ajay Mitra, Sudip Rangan, Latha Meta Gene Article Molecular genetic fingerprints of eleven Hedychium species from Northeast India were developed using PCR based markers. Fifteen inter-simple sequence repeats (ISSRs) and five amplified fragment length polymorphism (AFLP) primers produced 547 polymorphic fragments. Positive correlation (r = 0.46) was observed between the mean genetic similarity and genetic diversity parameters at the inter-species level. AFLP and ISSR markers were able to group the species according to its altitude and intensity of flower aroma. Cophenetic correlation coefficients between the dendrogram and the original similarity matrix were significant for ISSR (r = 0.89) compared to AFLP (r = 0.83) markers. This genetic characterization of Hedychium from Northeast India contributes to the knowledge of genetic structure of the species and can be used to define strategies for their conservation and management. Elsevier 2014-07-01 /pmc/articles/PMC4287868/ /pubmed/25606430 http://dx.doi.org/10.1016/j.mgene.2014.05.002 Text en © 2014 The Authors http://creativecommons.org/licenses/by-nc-nd/3.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/3.0/). |
spellingShingle | Article Basak, Supriyo Ramesh, Aadi Moolam Kesari, Vigya Parida, Ajay Mitra, Sudip Rangan, Latha Genetic diversity and relationship of Hedychium from Northeast India as dissected using PCA analysis and hierarchical clustering |
title | Genetic diversity and relationship of Hedychium from Northeast India as dissected using PCA analysis and hierarchical clustering |
title_full | Genetic diversity and relationship of Hedychium from Northeast India as dissected using PCA analysis and hierarchical clustering |
title_fullStr | Genetic diversity and relationship of Hedychium from Northeast India as dissected using PCA analysis and hierarchical clustering |
title_full_unstemmed | Genetic diversity and relationship of Hedychium from Northeast India as dissected using PCA analysis and hierarchical clustering |
title_short | Genetic diversity and relationship of Hedychium from Northeast India as dissected using PCA analysis and hierarchical clustering |
title_sort | genetic diversity and relationship of hedychium from northeast india as dissected using pca analysis and hierarchical clustering |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4287868/ https://www.ncbi.nlm.nih.gov/pubmed/25606430 http://dx.doi.org/10.1016/j.mgene.2014.05.002 |
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