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Genetic polymorphisms in miRNAs targeting the estrogen receptor and their effect on breast cancer risk

Breast cancer is the cancer that most commonly affects women worldwide. This type of cancer is genetically complex, but is strongly linked to steroid hormone signaling systems. Because microRNAs act as translational regulators of multiple genes, including the steroid nuclear receptors, single nucleo...

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Autores principales: Nguyen-Dien, Giang T., Smith, Robert A., Haupt, Larisa M., Griffiths, Lyn R., Nguyen, Hue T.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4287891/
https://www.ncbi.nlm.nih.gov/pubmed/25606406
http://dx.doi.org/10.1016/j.mgene.2014.01.002
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author Nguyen-Dien, Giang T.
Smith, Robert A.
Haupt, Larisa M.
Griffiths, Lyn R.
Nguyen, Hue T.
author_facet Nguyen-Dien, Giang T.
Smith, Robert A.
Haupt, Larisa M.
Griffiths, Lyn R.
Nguyen, Hue T.
author_sort Nguyen-Dien, Giang T.
collection PubMed
description Breast cancer is the cancer that most commonly affects women worldwide. This type of cancer is genetically complex, but is strongly linked to steroid hormone signaling systems. Because microRNAs act as translational regulators of multiple genes, including the steroid nuclear receptors, single nucleotide polymorphisms (SNPs) in microRNA genes can have potentially wide-ranging influences on breast cancer development. Thus, this study was conducted to investigate the relationships between six SNPs (rs6977848, rs199981120, rs185641358, rs113054794, rs66461782, and rs12940701) located in four miRNA genes predicted to target the estrogen receptor (miR-148a, miR-221, miR-186, and miR-152) and breast cancer risk in Caucasian Australian women. By using high resolution melt analysis (HRM) and polymerase chain reaction- restriction fragment length polymorphism (PCR–RFLP), 487 samples including 225 controls and 262 cases were genotyped. Analysis of their genotype and allele frequencies indicated that the differences between case and control populations were not significant for rs6977848, rs66461782, and rs12940701 because their p-values are 0.81, 0.93, and 0.1, respectively, which are all above the threshold value (p = 0.05). Our data thus suggests that these SNPs do not affect breast cancer risk in the tested population. In addition, rs199981120, rs185641358, and rs113054794 could not be found in this population, suggesting that these SNPs do not occur in Caucasian Australians.
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spelling pubmed-42878912015-01-20 Genetic polymorphisms in miRNAs targeting the estrogen receptor and their effect on breast cancer risk Nguyen-Dien, Giang T. Smith, Robert A. Haupt, Larisa M. Griffiths, Lyn R. Nguyen, Hue T. Meta Gene Article Breast cancer is the cancer that most commonly affects women worldwide. This type of cancer is genetically complex, but is strongly linked to steroid hormone signaling systems. Because microRNAs act as translational regulators of multiple genes, including the steroid nuclear receptors, single nucleotide polymorphisms (SNPs) in microRNA genes can have potentially wide-ranging influences on breast cancer development. Thus, this study was conducted to investigate the relationships between six SNPs (rs6977848, rs199981120, rs185641358, rs113054794, rs66461782, and rs12940701) located in four miRNA genes predicted to target the estrogen receptor (miR-148a, miR-221, miR-186, and miR-152) and breast cancer risk in Caucasian Australian women. By using high resolution melt analysis (HRM) and polymerase chain reaction- restriction fragment length polymorphism (PCR–RFLP), 487 samples including 225 controls and 262 cases were genotyped. Analysis of their genotype and allele frequencies indicated that the differences between case and control populations were not significant for rs6977848, rs66461782, and rs12940701 because their p-values are 0.81, 0.93, and 0.1, respectively, which are all above the threshold value (p = 0.05). Our data thus suggests that these SNPs do not affect breast cancer risk in the tested population. In addition, rs199981120, rs185641358, and rs113054794 could not be found in this population, suggesting that these SNPs do not occur in Caucasian Australians. Elsevier 2014-03-28 /pmc/articles/PMC4287891/ /pubmed/25606406 http://dx.doi.org/10.1016/j.mgene.2014.01.002 Text en © 2014 The Authors http://creativecommons.org/licenses/by-nc-nd/3.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/3.0/).
spellingShingle Article
Nguyen-Dien, Giang T.
Smith, Robert A.
Haupt, Larisa M.
Griffiths, Lyn R.
Nguyen, Hue T.
Genetic polymorphisms in miRNAs targeting the estrogen receptor and their effect on breast cancer risk
title Genetic polymorphisms in miRNAs targeting the estrogen receptor and their effect on breast cancer risk
title_full Genetic polymorphisms in miRNAs targeting the estrogen receptor and their effect on breast cancer risk
title_fullStr Genetic polymorphisms in miRNAs targeting the estrogen receptor and their effect on breast cancer risk
title_full_unstemmed Genetic polymorphisms in miRNAs targeting the estrogen receptor and their effect on breast cancer risk
title_short Genetic polymorphisms in miRNAs targeting the estrogen receptor and their effect on breast cancer risk
title_sort genetic polymorphisms in mirnas targeting the estrogen receptor and their effect on breast cancer risk
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4287891/
https://www.ncbi.nlm.nih.gov/pubmed/25606406
http://dx.doi.org/10.1016/j.mgene.2014.01.002
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