Specific recognition of guanines in non-duplex regions of nucleic acids with potassium tungstate and hydrogen peroxide

Structural features of nucleic acids have become an integral part of current biomedical research. Highly selective and readily performed methods with little toxicity that target guanosines in non-duplex nucleic acids are needed, which led us to search for an effective agent for guanosine sequencing....

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Autores principales: Mao, Wuxiang, Xu, Xiaowei, He, Huan, Huang, Rong, Chen, Xi, Xiao, Heng, Yu, Zhenduo, Liu, Yi, Zhou, Xiang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2015
Materias:
Methods Online
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4288145/
https://www.ncbi.nlm.nih.gov/pubmed/25355517
http://dx.doi.org/10.1093/nar/gku1025
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author Mao, Wuxiang
Xu, Xiaowei
He, Huan
Huang, Rong
Chen, Xi
Xiao, Heng
Yu, Zhenduo
Liu, Yi
Zhou, Xiang
author_facet Mao, Wuxiang
Xu, Xiaowei
He, Huan
Huang, Rong
Chen, Xi
Xiao, Heng
Yu, Zhenduo
Liu, Yi
Zhou, Xiang
author_sort Mao, Wuxiang
collection PubMed
description Structural features of nucleic acids have become an integral part of current biomedical research. Highly selective and readily performed methods with little toxicity that target guanosines in non-duplex nucleic acids are needed, which led us to search for an effective agent for guanosine sequencing. Treatment of DNA or RNA with potassium tungstate and hydrogen peroxide produced damaged guanosines in DNA or RNA sequences. The damaged guanosines in non-duplex DNA could be cleaved by hot piperidine. Similarly, damaged guanosines in non-duplex RNA could be cleaved by aniline acetate. We could identify structural features of nucleic acid using this strategy instead of dimethyl sulphate and Ribonuclease T1.
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spelling pubmed-42881452015-02-19 Specific recognition of guanines in non-duplex regions of nucleic acids with potassium tungstate and hydrogen peroxide Mao, Wuxiang Xu, Xiaowei He, Huan Huang, Rong Chen, Xi Xiao, Heng Yu, Zhenduo Liu, Yi Zhou, Xiang Nucleic Acids Res Methods Online Structural features of nucleic acids have become an integral part of current biomedical research. Highly selective and readily performed methods with little toxicity that target guanosines in non-duplex nucleic acids are needed, which led us to search for an effective agent for guanosine sequencing. Treatment of DNA or RNA with potassium tungstate and hydrogen peroxide produced damaged guanosines in DNA or RNA sequences. The damaged guanosines in non-duplex DNA could be cleaved by hot piperidine. Similarly, damaged guanosines in non-duplex RNA could be cleaved by aniline acetate. We could identify structural features of nucleic acid using this strategy instead of dimethyl sulphate and Ribonuclease T1. Oxford University Press 2015-01-09 2014-10-29 /pmc/articles/PMC4288145/ /pubmed/25355517 http://dx.doi.org/10.1093/nar/gku1025 Text en © The Author(s) 2014. Published by Oxford University Press on behalf of Nucleic Acids Research. http://creativecommons.org/licenses/by/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Methods Online
Mao, Wuxiang
Xu, Xiaowei
He, Huan
Huang, Rong
Chen, Xi
Xiao, Heng
Yu, Zhenduo
Liu, Yi
Zhou, Xiang
Specific recognition of guanines in non-duplex regions of nucleic acids with potassium tungstate and hydrogen peroxide
title Specific recognition of guanines in non-duplex regions of nucleic acids with potassium tungstate and hydrogen peroxide
title_full Specific recognition of guanines in non-duplex regions of nucleic acids with potassium tungstate and hydrogen peroxide
title_fullStr Specific recognition of guanines in non-duplex regions of nucleic acids with potassium tungstate and hydrogen peroxide
title_full_unstemmed Specific recognition of guanines in non-duplex regions of nucleic acids with potassium tungstate and hydrogen peroxide
title_short Specific recognition of guanines in non-duplex regions of nucleic acids with potassium tungstate and hydrogen peroxide
title_sort specific recognition of guanines in non-duplex regions of nucleic acids with potassium tungstate and hydrogen peroxide
topic Methods Online
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4288145/
https://www.ncbi.nlm.nih.gov/pubmed/25355517
http://dx.doi.org/10.1093/nar/gku1025
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