Mg(2+)-dependent conformational changes and product release during DNA-catalyzed RNA ligation monitored by Bimane fluorescence

Among the deoxyribozymes catalyzing the ligation of two RNA substrates, 7S11 generates a branched RNA containing a 2′,5′-linkage. We have attached the small fluorogenic probe Bimane to the triphosphate terminated RNA substrate and utilized emission intensity and anisotropy to follow structural rearr...

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Detalles Bibliográficos
Autores principales: Turriani, Elisa, Höbartner, Claudia, Jovin, Thomas M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2015
Materias:
Chemical Biology and Nucleic Acid Chemistry
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4288166/
https://www.ncbi.nlm.nih.gov/pubmed/25505142
http://dx.doi.org/10.1093/nar/gku1268

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4288166/
https://www.ncbi.nlm.nih.gov/pubmed/25505142
http://dx.doi.org/10.1093/nar/gku1268

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